• BMB Reports
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• v.37 no.2
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• pp.199-205
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• 2004

The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was $50^{\circ}C$, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six isoenzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 isoenzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.

• The Pure and Applied Mathematics
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• v.24 no.2
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• pp.109-127
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• 2017

In this paper, we solve the following quadratic ${\rho}-functional$ inequalities ${\parallel}f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z){\parallel}$ (0.1) ${\leq}{\parallel}{\rho}(f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\frac{1}{{\mid}4{\mid}}}$, and ${\parallel}f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z){\parallel}$ (0.2) ${\leq}{\parallel}{\rho}(f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\mid}8{\mid}$. Using the direct method, we prove the Hyers-Ulam stability of the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces and prove the Hyers-Ulam stability of quadratic ${\rho}-functional$ equations associated with the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces.

• Analytical Science and Technology
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• v.13 no.6
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• pp.712-721
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• 2000

$LiF-BeF_2-ZrF_4$(63-30-7 mol%) molten salt was dissolved up to 0.02g in 1ml of distilled water at room temperature. $ZrO_2$ oxide made from $ZrF_4$ through pyrohydrolysis was recovered by leaching in distilled water with $LiF-BeF_2-ZrF_4$molten salt including it at room temperature. The crystalline sharpness of recovered $ZrO_2$ oxide was not damaged.

• Journal of the Chungcheong Mathematical Society
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• v.22 no.4
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• pp.757-770
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• 2009

In this paper, we prove the generalized Hyers-Ulam stability of the following additive-quadratic-cubic-quartic functional equation (0.1) f(x + 2y) + f(x - 2y) = 4f(x + y) + 4f(x - y) - 6f(x) + f(2y) + f(-2y) - 4f(y) - 4f(-y) in Banach spaces.

• The Pure and Applied Mathematics
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• v.23 no.3
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• pp.247-263
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• 2016

Let $M_{1}f(x,y):=\frac{3}{4}f(x+y)-\frac{1}{4}f(-x-y)+\frac{1}{4}f(x-y)+\frac{1}{4}f(y-x)-f(x)-f(y)$, $M_{2}f(x,y):=2f(\frac{x+y}{2})+f(\frac{x-y}{2})+f(\frac{y-x}{2})-f(x)-f(y)$. Using the direct method, we prove the Hyers-Ulam stability of the additive-quadratic ρ-functional inequalities (0.1) $N(M_{1}f(x,y),t){\geq}N({\rho}M_{2}f(x,y),t)$ where ρ is a fixed real number with |ρ| < 1, and (0.2) $N(M_{2}f(x,y),t){\geq}N({\rho}M_{1}f(x,y),t)$ where ρ is a fixed real number with |ρ| < $\frac{1}{2}$.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.6
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• pp.391-397
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• 2010

E2F transcription factors and their target genes have been known to play an important role in cell growth control. We found that curcumin, a polyphenolic phytochemical isolated from the plant Curcuma longa, markedly suppressed E2F4 expression in HCT116 colon cancer cells. Hydrogen peroxide was also found to decrease E2F4 protein level, indicating the involvement of reactive oxygen species (ROS) in curucmin-induced downregulation of E2F4 expression. Involvement of ROS in E2F4 downregulation in response to curcumin was confirmed by the result that pretreatment of cells with N-acetylcystein (NAC) before exposure of curcumin almost completely blocked the reduction of E2F4 expression at the protein as well as mRNA level. Anti-proliferative effect of curcumin was also suppressed by NAC which is consistent to previous reports showing curcumin-superoxide production and induction of poly (ADP-ribose) polymerase (PARP) cleavage as well as apoptosis. Expression of several genes, cyclin A, p21, and p27, which has been shown to be regulated in E2F4-dependent manner and involved in the cell cycle progression was also affected by curcumin. Moreover, decreased (cyclin A) and increased (p21 and p27) expression of these E2F4 downstream genes by curcumin was restored by pretreatment of cells with NAC and E2F4 overexpression which is induced by doxycycline. In addition, E2F4 overexpression was observed to partially ameliorate curcumin-induced growth inhibition by cell viability assay. Taken together, we found curcumin-induced ROS down-regulation of E2F4 expression and modulation of E2F4 target genes which finally lead to the apoptotic cell death in HCT116 colon cancer cells, suggesting that E2F4 appears to be a novel determinant of curcumin-induced cytotoxicity.

• Journal of the Korean Vacuum Society
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• v.4 no.2
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• pp.119-123
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• 1995

Bridgman 방법으로 성장한 InSe: Co 단결정의 근적외 영역에서의 광흡수 특성을 상온에서 조사하였다. 1350, 1530, 1710nm 파장영역에서 Td 대칭을 갖는 Co2+ 이온의 4A2(4F)$\longrightarrow$4T1(4F)전이에 대응되는 3개의 흡수 peak를 관측하였다. 이 미세구조는 스핀-궤도 결합효과에 의하여 분리된 Co2+ 이온의 4T1(4F) 준위의 $\Gamma$6, $\Gamma$8, $\Gamma$7+$\Gamma$8 준위와 바닥상태 4A2(4F)의 $\Gamma$8 준위 사이의 전자전이에 기인하며, 결정장이론에 의하여 잘 설명되었다.

• The Pure and Applied Mathematics
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• v.23 no.2
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• pp.163-179
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• 2016

Let $M_1f(x,y):=\frac{3}{4}f(x+y)-\frac{1}{4}f(-x-y)+\frac{1}{4}(x-y)+\frac{1}{4}f(y-x)-f(x)-f(y)$, $M_2f(x,y):=2f(\frac{x+y}{2})+f(\frac{x-y}{2})+f(\frac{y-x}{2})-f(x)-f(y)$ Using the direct method, we prove the Hyers-Ulam stability of the additive-quadratic ρ-functional inequalities (0.1) $N(M_1f(x,y)-{\rho}M_2f(x,y),t){\geq}\frac{t}{t+{\varphi}(x,y)}$ and (0.2) $N(M_2f(x,y)-{\rho}M_1f(x,y),t){\geq}\frac{t}{t+{\varphi}(x,y)}$ in fuzzy Banach spaces, where ρ is a fixed real number with ρ ≠ 1.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.36-36
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• 2010

Low-pressure fluorocarbon plasmas are widely used in microelectronics fabrication for a variety of surface modification purposes. In particular, fluorocarbon plasmas are used for the etching of dielectrics such as silicon dioxide and silicon nitride. Among the various fluorocarbons, this study focuses on C4F6 molecules (C4F6s) which are composed of hexafluorocyclobutene (c-C4F6), hexafluoro-1, 3-butadiene (1, 3-C4F6), and hexafluoro-2-butyne (2-C4F6). We have investigated the dissociation reactions of C4F6s, resulting in CF2, CF3, C2F3, and C3F3 fragments, by using the wB97X-D functional with various basis sets. In this presentation, the geometrical properties, energetics, and dissociation mechanisms of C4F6s will be suggested.

• Journal of the Chungcheong Mathematical Society
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• v.30 no.4
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• pp.467-476
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• 2017

In this paper, we obtain the stability of the additive-quadratic functional equation f(x+y, z+w)+f(x+y, z-w) = 2f(x, z)+2f(x, w)+2f(y, z)+2f(y, w) and the bi-Jensen functional equation $$4f(\frac{x+y}{2},\;\frac{z+w}{2})=f(x,\;z)+f(x,\;w)+f(y,\;z)+f(y,\;w)$$ in 2-Banach spaces.