• Title/Summary/Keyword: F1 generation

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Estimating the Parameters of Pollen Flow and Mating System in Pinus densiflora Population in Buan, South Korea, Using Microsatellite Markers (Microsatellite 표지를 이용한 부안지역 소나무 집단의 화분 유동과 교배양식 추정)

  • Kim, Young Mi;Hong, Kyung Nak;Park, Yu Jin;Hong, Yong Pyo;Park, Jae In
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.101-110
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    • 2015
  • Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, were estimated using seven nuclear microsatellite markers. The expected heterozygosity ($H_e$) was 0.614 in mother trees and 0.624 in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between the generations (P > 0.05). From MLTR, the outcrossing rate ($t_m$), the biparental inbreeding ($t_m-t_s$), and the correlation of paternity ($r_p$) were 0.967, 0.057, and 0.012, respectively. tm was larger but $t_m-t_s$ and $r_p$ were smaller than those of allozyme markers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimal pollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and its level of genetic differentiation in pollen pool structure (${\Phi}_{ft}$) was 0.021. The average radial distance of pollen flow (${\delta}$) was calculated as 11.42 m, but no correlation between the pairwise-${\Phi}_{ft}$ and the geographical distance among mother trees was at Mantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, the amount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be because the genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the complete random mating condition in the Pinus densiflora population in Buan.

New Service Creation based on the Morphological Approach: Generation of Service Concept for Mobile Business (Morphology 접근법을 통한 신 서비스 창출 : 모바일 비즈니스의 서비스 개념 개발)

  • Choi Chang-U;Kim Cheol-Hyeon;Park Yong-Tae
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2006.05a
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    • pp.49-56
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    • 2006
  • 최근 제조업을 보완하는 새로운 성장동력으로 서비스산업의 중요성이 부각되고 있다. 특히 무선 인터넷과 통신 기술의 발전은 신 서비스 창출의 기회를 마련해주는 동시에 서비스 시스템을 복잡하게 변화시키고 있다. 그러나 기존의 서비스 개발은 정성적인 판단과 직관에 의지하고 있으며, 서비스 개발 방법론은 개념적이고 추상적인 수준에서 제공되고 있다는 한계를 가진다. 이는 모바일 비즈니스(mobile business)와 같은 복잡한 서비스 시스템의 변화에 능동적으로 대처하지 못하고 있어, 모바일 비즈니스를 위한 실질적이고 체계적인 신 서비스 개발의 방법론의 필요성을 증가시킨다. 본 연구에서는 morphology 분석을 통해 모바일 비즈니스를 위한 체계적인 신서비스 개발 방법론을 제안한다. 특히 새로운 무선 통신 환경아래에서 급격한 변화를 맞고 있는 음악 산업을 대상으로 제안된 방법론의 활용 사례를 제시할 것이다. 제안된 방법론은 크게 4 단계로 구성된다. 첫째, 제공되는 서비스 시스템을 체계화하기 위해 morphology의 dimension들을 정의한다. 각 차원은 서비스 시스템의 하위 시스템이며, 이는 기존의 문헌조사와 연구를 통해 정의된다. 둘째, 각 차원별로 morphology의 shape를 정의한다. 셋째, 각 차원의 shape를 조합하여 과거의 서비스 시스템을 표현하고, morphology의 dimension과 shape를 확장한다. 넷째, morphology의 확장된 dimension과 shape를 조합하여 새로운 서비스를 구성한다. 제안된 접근법은 크게 두 가지 측면에서 강점을 가진다. 첫째, 신 서비스 아이디어의 획득을 용이하게 할 수 있다. morphology분석은 체계적인 설계 방법론을 창의적인 생각을 돕는 도구로 의미가 있다. 둘째, 체계적인 신 서비스의 개발을 가능하게 한다. morphology접근법은 M-비즈니스와 같은 복잡한 서비스 시스템을 구조적으로 분석하고, 개발할 수 있는 기틀이 된다.킹 대상을 도출할 때까지 추가적인 분석과정을 반복한다. 제안한 방법을 통하여 조직은 기술적 생산 가능성 외에도 다양한 조직 운영 관점에서 적절한 벤치마킹 대상을 선정할 수 있으며, 이에 따른 목표를 수립할 수 있을 것으로 기대한다. 또한 더 나아가 global efficiency 관점에서 효율적 조직이 되기 위하여 단계적인 벤치마킹 대상 선정과 이에 따른 목표를 수립하는데도 유용하리라 판단된다.$1.20{\pm}0.37L$, 72시간에 $1.33{\pm}0.33L$로 유의한 차이를 보였으므로(F=6.153, P=0.004), 술 후 폐환기능 회복에 효과가 있다. 4) 실험군과 대조군의 수술 후 노력성 폐활량은 수술 후 72시간에서 실험군이 $1.90{\pm}0.61L$, 대조군이 $1.51{\pm}0.38L$로 유의한 차이를 보였다(t=2.620, P=0.013). 5) 실험군과 대조군의 수술 후 일초 노력성 호기량은 수술 후 24시간에서 $1.33{\pm}0.56L,\;1.00{\ge}0.28L$로 유의한 차이를 보였고(t=2.530, P=0.017), 술 후 72시간에서 $1.72{\pm}0.65L,\;1.33{\pm}0.3L$로 유의한 차이를 보였다(t=2.540, P=0.016). 6) 대상자의 술 후 폐환기능에 영향을 미치는 요인은 성별로 나타났다. 이에 따른 폐환기능의 차이를 보면, 실험군의 술 후 노력성 폐활량이 48시간에 남자($1.78{\pm}0.61L$)가 여자($1.27{\pm}0.45L$)보다 더 높게 나타났으며 (t=2.170, P=0.042), 72시간에도 역시 남자($2.16{\pm}0.56L$)가 여자($1.50{\pm}0.47L$)보다 더 높았다(t=2.910, P=0.008). 술 후 일초 노

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THE INFLUENCE OF IRM TEMPORARY RESTORATIONS ON MARGINAL MICROLEAKAGE OF DENTIN ADHESIVES (IRM 임시수복이 상아질 접착제의 변연 미세누출에 미치는 영향)

  • Cho, Young-Gon;Kim, Hyun-Kyung;Lee, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.28 no.1
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    • pp.1-10
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    • 2003
  • This study investigated the influence of IRM on marginal microleakage of 5th generation adhesives. Class V cavities with gingival margins in dentin were prepared on both buccal and lingual surfaces of 60 extract-ed human molar teeth. Prepared teeth were randomly divided into six groups. Group 1 and 4 received no temporary restoration with IRM. Group 2 and 5 were covered with IRM mixed at P/L ratio(10g/1g). Group 3 and 6 were covered with IRM mixed at P/L ratio(10g/2g). The temporary restorations were removed mechanically with an ultrasonic scaler after one-week storage in distilled water. The cavities were restored using one of two adhesives and composites ; Single Bond/Filtek Z 250(Croup 1, 2 and 3), UniFil Bond/UniFil F(Group 4, 5 and 6). Following one day storage in distilled water, the restored teeth were thermocycled for 500 cycles(between $5^{\circ}C{\;}and{\;}55^{\circ}C$) and immersed in 2 % methylene blue for dye penetration testing. The results were analysed using Kruskal-Wallis Test, Mann-Whitney and Wilcoxon signed ranked test at a significance level of 0.05. The results of this study were as follows 1. Ranking of mean microleakage scores at the enamel margins was Group 10.05) 4. At the dentin margins, the microleakage of the group not pretreated with IRM was lower than that of the group pretreated with IRM. And the microleakage of UniFil Bond was lower than that of Single Bond. 5. Compared with microleakages between the enamel and dentin margins of each groups, Group 1, 2, 3, 4, 5 and 6 at dentin margin were higher microleakage than those at enamel margin. There were significant difference between enamel and dentin microleakage of Group 2 and 3(p<0.05).

A New Tongil-type Glutinous Rice Variety 'Hangangchal 1' of Multi-Diseases and Insect Resistance (중생 복합내병성 통일형 찰벼 품종 '한강찰 1호')

  • Song, You-Chun;Cho, Jun-Hyeon;Jung, Kuk-Hyun;Ha, Woon-Goo;Kim, Se-Ri;Kwak, Do-Yeon;Park, No-Bong;Kim, Young-Doo;Kim, Sang-Yeol;Oh, Seong-Hwan;Lim, Sang-Jong;Shin, Mun-Sik
    • Korean Journal of Breeding Science
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    • v.43 no.3
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    • pp.201-205
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    • 2011
  • 'Hangangchal 1' is a new glutinous rice cultivar of second generation Tongil-type with a mid-maturing ecotype that developed by the rice breeding team of Yeongnam Agricultural Research Institute (YARI), RDA. in 2006. This cultivar was derived from a cross between 'Hangangchal', a Tongil-type glutinous cultivar and 'YR8208-20', a high yield potential in 1986/1987 winter season. 'Hangangchal 1' was selected by pedigree and bulk breeding methods from $F_3$ to $F_6$ populations. A promising line, YR10498-8-1-3, was selected and designated as 'Milyang 167' in 1997. The local adaptability test of 'Milyang 167' was carried out at seven locations during 3 years in 1998, 2005, and 2006. It has tolerance to lodging with good canopy architecture as 87cm of culm length. This cultivar is resistant to bacterial blight $K_1$, $K_2$, and $K_3$ race, rice stripe virus, rice dwarf virus, and leaf blast disease. The milled rice endosperm of 'Hangangchal 1' is glutinous and its whiteness was almost similar compared to 'Shinseonchalbyeo'. The yield of milled rice of 'Hangangchal 1' was average 5.97 MT/ha at ordinary cultivation of 9 kg/10a N fertilizer level in local adaptability test. This cultivar would be adaptable to the mid and southern plain of Korea.

Hydrogeochemical Evolution Related to High Fluoride Concentrations in Deep Bedrock Groundwaters, Korea (국내 심부 암반지하수에서의 고농도 불소 산출과 관련된 수리지구화학 진화)

  • Kim Kyoung-Ho;Yun Seong-Taek;Chae Gi-Tak;Kim Seong-Yong;Kwon Jang-Soon;Koh Yong-Kwon
    • Economic and Environmental Geology
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    • v.39 no.1 s.176
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    • pp.27-38
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    • 2006
  • To understand the geologic and hydrogeochemical controls on the occurrence of high fluoride concentrations in bedrock groundwaters of South Korea, we examined a total of 367 hydrochemistry data obtained from deep groundwater wells (avg. depth=600 m) that were drilled fur exploitation of hot springs. The fluoride concentrations were generally very high (avg. 5.65mg/L) and exceeded the Drinking Water Standard (1.5 mg/L) in $72\%$ of the samples. A significant geologic control of fluoride concentrations was observed: the highest concentrations occur in the areas of granitoids and granitic gneiss, while the lowest concentrations in the areas of volcanic and sedimentary rocks. In relation to the hydrochemical facies, alkaline $Na-HCO_3$ type waters had remarkably higher F concentrations than circum-neutral to slightly alkaline $Ca-HCO_3$ type waters. The prolonged water-rock interaction occurring during the deep circulation of groundwater in the areas of granitoids and granitic gneiss is considered most important for the generation of high F concentrations. Under such condition, fluoride-rich groundwaters are likely formed through hydrogeochemical processes consisting of the removal of Ca from groundwater via calcite precipitation and/or cation exchange and the successive dissolution of plagioclase and F-bearing hydroxyl minerals (esp. biotite). Thus, groundwaters with high pH and very high Na/Ca ratio within granitoids and granitic gneiss are likely most vulnerable to the water supply problem related to enriched fluorine.

Regeneration of a defective Railroad Surface for defect detection with Deep Convolution Neural Networks (Deep Convolution Neural Networks 이용하여 결함 검출을 위한 결함이 있는 철도선로표면 디지털영상 재 생성)

  • Kim, Hyeonho;Han, Seokmin
    • Journal of Internet Computing and Services
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    • v.21 no.6
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    • pp.23-31
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    • 2020
  • This study was carried out to generate various images of railroad surfaces with random defects as training data to be better at the detection of defects. Defects on the surface of railroads are caused by various factors such as friction between track binding devices and adjacent tracks and can cause accidents such as broken rails, so railroad maintenance for defects is necessary. Therefore, various researches on defect detection and inspection using image processing or machine learning on railway surface images have been conducted to automate railroad inspection and to reduce railroad maintenance costs. In general, the performance of the image processing analysis method and machine learning technology is affected by the quantity and quality of data. For this reason, some researches require specific devices or vehicles to acquire images of the track surface at regular intervals to obtain a database of various railway surface images. On the contrary, in this study, in order to reduce and improve the operating cost of image acquisition, we constructed the 'Defective Railroad Surface Regeneration Model' by applying the methods presented in the related studies of the Generative Adversarial Network (GAN). Thus, we aimed to detect defects on railroad surface even without a dedicated database. This constructed model is designed to learn to generate the railroad surface combining the different railroad surface textures and the original surface, considering the ground truth of the railroad defects. The generated images of the railroad surface were used as training data in defect detection network, which is based on Fully Convolutional Network (FCN). To validate its performance, we clustered and divided the railroad data into three subsets, one subset as original railroad texture images and the remaining two subsets as another railroad surface texture images. In the first experiment, we used only original texture images for training sets in the defect detection model. And in the second experiment, we trained the generated images that were generated by combining the original images with a few railroad textures of the other images. Each defect detection model was evaluated in terms of 'intersection of union(IoU)' and F1-score measures with ground truths. As a result, the scores increased by about 10~15% when the generated images were used, compared to the case that only the original images were used. This proves that it is possible to detect defects by using the existing data and a few different texture images, even for the railroad surface images in which dedicated training database is not constructed.

Production of the melittin antimicrobial peptide in transgenic silkworm (멜리틴 항균펩타이드를 생산하는 형질전환누에)

  • Kim, Seong Wan;Goo, Tae Won;Kim, Seong Ryul;Park, Seung Won;Choi, Kwang-Ho
    • Journal of Sericultural and Entomological Science
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    • v.53 no.1
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    • pp.55-60
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    • 2015
  • Melittin is the main component of Bee Venom and has antibacterial activity against several bacteria. To produce the melittin antimicrobial peptide, we constructed transgenic silkworm that expressed melittin gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 300 eggs of bivoltin silkworms, Baegokjam. In total, 131 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 36 broods, and we selected 4 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 11%. We identified 12 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a melittin as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that melittin possesses high antibacterial activities against gramnegative bacteria.

A STUDY ON THE SOLUBILITY AND THE WATER SORPTION OF VARIOUS RESIN CEMENTS (수종의 레진 시멘트의 용해도와 수분흡착에 관한 연구)

  • Hwang Yu-Jin;Cho In-Ho;Lim Ju-Hwan;Lim Heon-Song
    • The Journal of Korean Academy of Prosthodontics
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    • v.43 no.1
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    • pp.1-14
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    • 2005
  • Statement of problem. Among the physical properties of adhesion luting cement, the aspect that requires the most important factor is the degree of solubility and water sorption. Dissolution or an inadequate due to excessive water sorption inside the oral cavity compromises the while concurrently increasing the susceptibility to secondary dental caries. Susceptibility to dissolution and difficulty of removing remnant cement from the gingival sulcus have hindered the use of dental resin cement in the clinical practice, but the improved characteristics of newer generation resin cements have interest in and enabled resin cements to be widely used in adhesion of fixed prosthesis, such as laminate veneers and all-ceramic crowns. Purpose. The purpose of this study is to compare and analyze the degrees of solubility and water sorption of a variety of resin cements widely used for clinical purposes with different curing methods. Material and methods. Self-curing resin cements, $Avanto^{(R)}$, $C&B^{TM}$ CEMENT and Superbond C&B cements comprised group 1, 2 and 3. The dual-curing resin cements $Panavian^{TM}$ F, $Calibra^{(R)}$ and $Variolink^{(R)}$ II were divided into groups 4, 5, and 6, respectively. The investigation was carried out using disc-shaped specimens as specified by ANSI/ADA Specification No. 27. The degree of water sorption, water solubility and lactic acid solubility of each test group was analyzed statistically leading to the following conclusion. Results. The degree of water sorption was shown to increase in the following order : group 6, 5, 4, 2, 1 and 3. There were significant differences between the water sorption of each group. Results of the degree of water solubility were shown to increase in the following order : group 6, 5, 4, 2, 1 and 3. Statistically significant differences were found between each group, with the exception of groups 1 and 3. Finally, the degree of lactic acid solubility was found to increase in the following order : group 6,5,4,2,3 and 1. Significant differences were found between each group. In general dual-curing resin cements displayed substantially lower values than self-curing resin cements with regard to water sorption, water solubility, and lactic acid solubility. Conclusions. From the results of this study, dual-curing resin cements show a significantly lower degree of water sorption and solubility than their self-curing counterparts. Clinically, when selecting resin cements, the product with a lower degree of water sorption and solubility are preferred. The results of this study indicate that the use-of dual-curing resin cements is preferable to self-curing cements.

'Hyowon6', a Mid-Late Maturing Rice Variety with High Quality (고품질 중만생 벼 품종 '효원6호')

  • Im, Da-Eun;San, Mar Lar;Jang, Seong-Gyu;Park, So-Yeon;Jin, Sang-Hyeon;Kim, Yong Chul;Ham, Tae-Ho;Kwon, Soon-Wook
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.529-533
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    • 2018
  • Hyowon6, a mid-late maturing, good eating quality rice variety with multi-resistance, was developed by the rice breeding team of Pusan National University in 2013. This variety was derived from a cross between Hwayeong (as a multi-resistant parent) and Koshihikari (as a good eating quality parent). Selection by the pedigree breeding method was carried out until the $F_8$ generation. A promising line, JS14-12-36-8-5-3-1-1-1, was developed and designated as HY103 in 2013. This variety headed on August 22, which was 11 days later than that of Hwayeong. The culm length and panicle length of Hyowon6 was 85.7 cm and 21.1 cm, respectively. The number of panicles per hill was 14.5 and the number of grains per panicle was 101.7. The ratio of ripened grain was approximately 92% and the 1,000-grain weight was approximately 21.36 g for brown rice, which were similar to those of Hwayeong. Hyowon6 was moderately resistant to lodging and also to neck blast, leaf blight, and stripe virus. The glossiness value of Hyowon6 was 83, which was considerably higher than that of Hwayeong.

Inhibition of Adipocyte Differentiation by Methanol Extracts of Oenanthe javanica Seed in 3T3-L1 Preadipocytes (돌미나리씨 추출물에 의한 3T3-L1 지방전구세포의 분화 억제)

  • Ji, Hyang Hwa;Jeong, Hyun Young;Jin, Soojung;Kwon, Hyun Ju;Kim, Byung Woo
    • Journal of Life Science
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    • v.22 no.12
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    • pp.1688-1696
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    • 2012
  • Oenanthe javanica has been used as a food source and also in traditional folk medicine for its detoxifying properties and anti-microbial effects since ancient times. In this study, we evaluated the effect and mechanism of O. javanica seed methanol extract (OJSE) on adipocyte differentiation by 3T3-L1 preadipocytes. Under non-toxic conditions, OJSE treatment resulted in a dose-dependent inhibition of lipid droplet generation and triglyceride accumulation by suppressing adipocyte differentiation, which are associated with the decreased expression of key proadipogenic transcription factors including CCAAR/enhancer binding protein ${\alpha}$, ${\beta}$ ($C/EBP{\alpha}$, $C/EBP{\beta}$) and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$). OJSE also significantly inhibited proliferation and differentiation of 3T3-L1 preadipocytes through G1-phase arrest, indicating that OJSE blocked mitotic clonal expansion during adipocyte differentiation. Investigation of the alteration of G1 phase arrest-related proteins indicated a dose-dependent increase in the expression of p21 and reduction in expression of cyclin E, Cdk2, E2F-1 and phospho-Rb by OSJE. Taken together, these results suggest that OJSE inhibits adipocyte differentiation by blocking the mitotic clonal expansion, which is accompanied by preadipocyte cell cycle arrest.