• Communications of the Korean Mathematical Society
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• v.37 no.4
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• pp.1025-1039
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• 2022

For given non-negative real numbers 𝛼_k with ∑^m_k=1 𝛼_k = 1 and normalized analytic functions f_k, k = 1, …, m, defined on the open unit disc, let the functions F and Fn be defined by F(z) := ∑^m_k=1 𝛼_kf_k(z), and F_n(z) := n^-1 ∑^n-1_j=0 e^-2j𝜋i/nF(e^2j𝜋i/nz). This paper studies the functions f_k satisfying the subordination zf'_k(z)/F_n(z) ≺ h(z), where the function h is a convex univalent function with positive real part. We also consider the analogues of the classes of starlike functions with respect to symmetric, conjugate, and symmetric conjugate points. Inclusion and convolution results are proved for these and related classes. Our classes generalize several well-known classes and the connections with the previous works are indicated.

• Transactions of the Korean Society of Mechanical Engineers
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• v.9 no.4
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• pp.430-439
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• 1985

Conjugate natural convection in double enclosed annuli between horizontal concentric cylinders has been studied by the numerical analysis and experimental measurements. The interface conditions between the liquid and the solid of middle shell are obtained through the correlation factor based on the ratio of solid to fluid thermal conductivities and the Prandtl number. The characteristics of conjugate heat transfer are discussed under various dimensionless parameters such as conductivity ratios, shell thickness, diameter ratios, Prandtl number, and Rayleigh number. It is found that the average equivalent conductivity K over var $_{eq}$ does not depend on the conductivity ratios and shell thickness. The K over bar $_{eq}$ however, depends on the Prandtl number and the Rayleigh number.

• Bulletin of the Korean Mathematical Society
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• v.42 no.4
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• pp.807-815
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• 2005

Let R be a ring with identity, X the set of all nonzero, nonunits of Rand G the group of all units of R. We will consider two group actions on X by G, the regular action and the conjugate action. In this paper, by investigating two group actions we can have some results as follows: First, if G is a finitely generated abelian group, then the orbit O(x) under the regular action on X by G is finite for all nilpotents x $\in$ X. Secondly, if F is a field in which 2 is a unit and F $\backslash\;\{0\}$ is a finitley generated abelian group, then F is finite. Finally, if G in a unit-regular ring R is a torsion group and 2 is a unit in R, then the conjugate action on X by G is trivial if and only if G is abelian if and only if R is commutative.

• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.205-209
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• 1982

The enzyme-linked immunosorbent assay using the so-called "double-sandwich"technique was applied to determine Clostridium botulinum type F toxin. Polystyrene tubes were coated with horse anti-type F toxin serum and then toxin sample was added. The tubes were subsequently treated with rabbit anti-type F toxin IgG and sheep anti-rabbit serum IgG-horseradish peroxidase conjugate. By this technique, about 10 mouse intraperitoneal 50% lethal doses (ip LD/50/) of type F toxin could be detected. Low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin as diluents of rabbit IgG and conjugate. Addition of EDTA in the diluents of toxin increased ELISA extinction value significantly. No cross-reaction was observed with botulinum type A and B toxin, but type E toxin gave sleight cross-reaction.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2113-2120
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• 2018

Cross-reactive material 197 ($CRM_{197}$) is a non-toxic mutant of diphtheria toxin containing a single amino acid substitution of glycine 52 with glutamic acid. $CRM_{197}$ has been used as a carrier protein for poorly immunogenic polysaccharide antigens to improve immune responses. In this study, to develop a sandwich ELISA that can detect $CRM_{197}$ and $CRM_{197}$ conjugate vaccines, we generated a human anti-$CRM_{197}$ monoclonal antibody (mAb) 3F9 using a phage-displayed human synthetic Fab library and produced mouse anti-$CRM_{197}$ polyclonal antibody. The affinity ($K_D$) of 3F9 for $CRM_{197}$ was 3.55 nM, based on Bio-Layer interferometry, and it bound specifically to the B fragment of $CRM_{197}$. The sandwich ELISA was carried out using 3F9 as a capture antibody and the mouse polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA was <1 ng/ml $CRM_{197}$. In addition, the 3F9 antibody bound to the $CRM_{197}$-polysaccharide conjugates tested in a dose-dependent manner. This ELISA system will be useful for the quantification and characterization of $CRM_{197}$ and $CRM_{197}$ conjugate vaccines. To our knowledge, this study is the first to generate a human monoclonal antibody against $CRM_{197}$ and to develop a sandwich ELISA for $CRM_{197}$ conjugate vaccines.

• Clinical and Experimental Reproductive Medicine
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• v.17 no.1
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• pp.87-91
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• 1990

Development of a solid-phase chemiluminescence immunoassay for the detection of progesterone in serum extract was described. The chemiluminescence immunoassay was establised utilizing anti-progesterone monoclonal antibody that coated on polystyrene tubes and progesterone-ABEI conjugate as tracer. The light yield generated from antibody bound conjugate was counted on clinilumat luminometer by oxidation with microperoxidase and peroxide. The chemiluminescence immunoassay was high specific and accurate and detects as little as 3.9ng/ml of progesterone. The intra-assay CV ranged from 6% to 11.5% and inter-assay CV ranged from 13.6% to 18.7%. This assay system was good correlated with conventional kit radioimmunoassay system (r=0.98).

• Infection and chemotherapy
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• v.50 no.4
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• pp.328-339
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• 2018

Background: Pneumococcal disease is a major cause of morbidity and mortality worldwide, especially in patients with comorbidities and advanced age. This study evaluated trends in epidemiology of adult pneumococcal disease in Crete, Greece, by identifying serotype distribution and antimicrobial resistance of consecutive Streptococcus pneumoniae strains isolated from adults during an 8-year time period (2009-2016) and the indirect effect of the infant pneumococcal higher-valent conjugate vaccines 10-valent pneumococcal conjugate vaccine (PCV10) and 13-valent pneumococcal conjugate vaccine (PCV13). Materials and Methods: Antimicrobial susceptibility was performed by E-test and serotyping by Quellung reaction. Multidrug resistance (MDR) was defined as non-susceptibility to penicillin (PNSP) combined with resistance to ${\geq}2$ non-${\beta}$-lactam antimicrobials. Results: A total of 135 S. pneumoniae strains were isolated from adults during the study period. Twenty-one serotypes were identified with 17F, 15A, 3, 19A, and 11A, being the most common. The coverage rates of PCV10, and PCV13 were 17.8% and 37.8%, respectively. PCV13 serotypes decreased significantly from 68.4% in 2009 to 8.3% in 2016 (P = 0.002). The most important emerging non-PCV13 serotypes were 17F, 15A, and 11A, with 15A being strongly associated with antimicrobial resistance and MDR. Among all study isolates, penicillin-resistant and MDR strains represented 7.4% and 14.1%, respectively. Predominant PNSP serotypes were 19A (21.7%), 11A (17.4%), and 15A (17.4%). Erythromycin, clindamycin, tetracycline, trimethoprim-sulfamethoxazole, and levofloxacin resistant rates were 30.4%, 15.6%, 16.3%, 16.3%, and 1.5%, respectively. Conclusion: Although pneumococcal disease continues to be a health burden in adults in Crete, our study reveals a herd protection effect of the infant pneumococcal higher-valent conjugate vaccination. Surveillance of changes in serotype distribution and antimicrobial resistance among pneumococcal isolates are necessary to guide optimal prevention and treatment strategies.

• Clinical and Experimental Pediatrics
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• v.54 no.4
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• pp.163-168
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• 2011

Purpose: The purpose of this study was to evaluate the immune response to serotype 19A in children aged 12-23 months after immunization of the 19F containing 7-valent pneumococcal conjugate vaccine (PCV7). Methods: Blood samples from a total of 45 subjects (age 12-23 months) were included in the study. Subjects were categorized according to immunization status into three groups as follows: 18 subjects with 3 primary doses and 1 booster dose of PCV7 (booster group), 21 subjects with 3 primary doses before 12 months of age (primary group), and 6 subjects with no vaccination history of PCV7 (control group). An ELISA and opsonophagocytic killing assay (OPKA) was done to evaluate the immune responses against serotypes 19F and 19A. Results: According to the ELISA, all subjects had antibody titers ${\geq}0.35{\mu}g/mL$ for serotypes 19F and 19A in the booster and primary group and 83.0% and 66.7% in the control group, respectively. According to the OPKA, subjects with opsonic activity (${\geq}20$) against serotypes 19F and 19A were 100% and 61.1% of the subjects in the booster group and 66.7% and 19.0% in the primary group, respectively. No subjects in the control group had opsonic antibodies against both serotypes. Conclusion: In conclusion, in children 12-23 months age who were previously vaccinated with PCV7, a cross-reactive immune response is elicited against serotype 19A after a primary series of 3 doses in a small proportion of subjects, and this response is amplified after booster vaccination.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.12
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• pp.1715-1725
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• 1998

A two-dimensional transient inverse heat conduction problem involving the estimation of the unknown location, ($X^*$, $Y^*$), and timewise varying unknown strength, $G({\tau})$, of a line heat source embedded inside a rectangular bar with insulated boundaries has been solved simultaneously. The regular conjugate gradient method, RCGM and the modified conjugate gradient method, MCGM with adjoint equation, are used alternately to estimate the unknown strength $G({\tau})$ of the source term, while the parameter estimation approach is used to estimate the unknown location ($X^*$, $Y^*$) of the line heat source. The alternate use of the regular and the modified conjugate gradient methods alleviates the convergence difficulties encountered at the initial and final times (i.e ${\tau}=0$ and ${\tau}={\tau}_f$), hence stabilizes the computation and fastens the convergence of the solution. In order to examine the effectiveness of this approach under severe test conditions, the unknown strength $G({\tau})$ is chosen in the form of rectangular, triangular and sinusoidal functions.

• Journal of the Korean Mathematical Society
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• v.34 no.2
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• pp.383-393
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• 1997

In this paper, we study the dependence of corestriction (or transfer) map on the choice of transversals. We also study transfer theorems with respect to some commutative subgroups.