Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 10 Issue 3
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- Pages.205-209
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- 1982
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Clostridium botulinum Type F Toxin
Clostridium botulinum Type F Toxin의 면역학적 효소방법에 의한 검출에 관한 연구
- Lee, Jeong-Kug (Korea Advanced Institute of Science and Technology) ;
- K. H. Yang (Korea Advanced Institute of Science and Technology)
- Published : 1982.09.01
Abstract
The enzyme-linked immunosorbent assay using the so-called "double-sandwich"technique was applied to determine Clostridium botulinum type F toxin. Polystyrene tubes were coated with horse anti-type F toxin serum and then toxin sample was added. The tubes were subsequently treated with rabbit anti-type F toxin IgG and sheep anti-rabbit serum IgG-horseradish peroxidase conjugate. By this technique, about 10 mouse intraperitoneal 50% lethal doses (ip LD/50/) of type F toxin could be detected. Low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin as diluents of rabbit IgG and conjugate. Addition of EDTA in the diluents of toxin increased ELISA extinction value significantly. No cross-reaction was observed with botulinum type A and B toxin, but type E toxin gave sleight cross-reaction.
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