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Effect of Different Volume of Microdrop Culture on B6D2F1 Mice Oogenesis (배양액 용량이 B6D2F1 마우스 배아발생능력에 미치는 영향)

  • Yoo, Chang-Seok;Park, Kee Sang;Seo, Byoung Boo
    • Journal of Embryo Transfer
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    • v.31 no.1
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    • pp.27-32
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    • 2016
  • This study was conducted to evaluate the effects of different volume ($100{\mu}l$ vs. 2 ml) of microdrop culture on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri $F_1$ mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. Blastulation rate was not different between groups ($58.4{\pm}2.9%$ vs. $61.2{\pm}4.8%$). Zona hatched rate ($38{\pm}15.4%$ vs. $27{\pm}3.4%$) and attached rate ($55{\pm}13.9%$ vs. $46{\pm}3.9%$) did not differ by the volume of culture media. Total cell numbers ($59.8{\pm}9.7$ vs. $70.3{\pm}8.7$), ICM cell numbers ($15.8{\pm}0.6$ vs. $16.8{\pm}1.5$), TE cell numbers ($44.0{\pm}9.7$ vs. $53.6{\pm}7.3$), % ICM ($26.4{\pm}2.9%$ vs. $23.8{\pm}3.3%$) and ICM:TE ratio ($1:2.8{\pm}0.4$ vs. $1:3.2{\pm}0.6$) were not different between groups (i.e., $100{\mu}l$ vs. 2 ml). These results show that the capacity of the culture medium did not effect the cell numbers of B6D2F1 mice blastocysts. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.

A new forma of Cremastra appendiculata Makino from Korea

  • Kil, Bong-Seop
    • Plant Resources
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    • v.3 no.3
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    • pp.223-225
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    • 2000
  • A new forma, Cremastra appendiculta Makino f. albiflora B. Kil, f. nov. is described from Naejangsan, Jeollabuk-do, Korea. It is easily distinguished from C. appendiculata by having light white-green flower of outer perianth segment and white color of inner perianth segment of the flower.

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Quality properties on soy bean pastes made with microorganisms isolated from traditional soy bean pastes (전통된장으로부터 분리한 균종으로 제조된 된장의 품질특성)

  • Yoo, Seung-Ku;Kang, Su-Min;Noh, Young-Sook
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1266-1270
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    • 2000
  • Quality properties of soy bean pastes made with Aspergillus oryzae and 5 Bacillus strains isolated from traditional soy bean pastes were examined. The pH decreased gradually and contents of amino-type nitrogen increased during fermentation. There were small differences in moisture and crude-protein contents, whereas big difference was observed in reducing sugar, isoflavones and organic acid contents. Isoflavones in the samples made with Bacillus licheniformis F2358 and Bacillus subtilis F2362 were high. Samples made with Bacillus licheniformis F2382 had high contents of organic acid and good score for taste and overall acceptability in sensory evaluation.

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Genetic Analysis of Taste of Korean Hot Pepper (한국 고추맛의 유전 분석)

  • Soh, Jae-Woo;Cho, Chi-Woong
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.1-8
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    • 2012
  • This study was carried out to determine the genic values of taste of Korean hot pepper (Capsicum annuum L.) in practical genetic resources of using to breed them. The two breeding materials of pepper, '#1803' ($P_1$) of prefer tastes and '#1532' ($P_2$) of ordinary taste, and their $F_1$, $F_2$ generations were used in this study. By using partitioning method it was possible to estimate, from the $F_2$ generation, the number of effective factor pairs differentiating the two parents. There were found to be differentiated by two effective factor pairs. In practical genetic resource of using to breed the Korean hot pepper, the heritance of pepper tastes showed that the $F_1$ was better than excellent parent by reason of over dominant, but some $F_2$ was better than both parent by transgressive segregation. As the result, the magnitude of genic effects of A-a gene in pepper tastes was 0.36, and B-b gene was 0.64. The tastes of Korean hot pepper showed a complex inheritance by interaction effect on the two non-allelic factors of 0.94 and secondary effect of 2.86 at the most.

A PROPERTY OF COFUNCTORS SF(X,A)

  • So, Kwang Ho
    • Kyungpook Mathematical Journal
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    • v.13 no.2
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    • pp.235-240
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    • 1973
  • A k-dimensional vector bundle is a bundle ${\xi}=(E,P,B,F^k)$ with fibre $F^k$ satisfying the local triviality, where F is the field of real numbers R or complex numbers C ([1], [2] and [3]). Let $Vect_k(X)$ be the set consisting of all isomorphism classes of k-dimensional vector bundles over the topological space X. Then $Vect_F(X)=\{Vect_k(X)\}_{k=0,1,{\cdots}}$ is a semigroup with Whitney sum (${\S}1$). For a pair (X, A) of topological spaces, a difference isomorphism over (X, A) is a vector bundle morphism ([2], [3]) ${\alpha}:{\xi}_0{\rightarrow}{\xi}_1$ such that the restriction ${\alpha}:{\xi}_0{\mid}A{\longrightarrow}{\xi}_1{\mid}A$ is an isomorphism. Let $S_k(X,A)$ be the set of all difference isomorphism classes over (X, A) of k-dimensional vector bundles over X with fibre $F^k$. Then $S_F(X,A)=\{S_k(X,A)\}_{k=0,1,{\cdots}}$, is a semigroup with Whitney Sum (${\S}2$). In this paper, we shall prove a relation between $Vect_F(X)$ and $S_F(X,A)$ under some conditions (Theorem 2, which is the main theorem of this paper). We shall use the following theorem in the paper. THEOREM 1. Let ${\xi}=(E,P,B)$ be a locally trivial bundle with fibre F, where (B, A) is a relative CW-complex. Then all cross sections S of ${\xi}{\mid}A$ prolong to a cross section $S^*$ of ${\xi}$ under either of the following hypothesis: (H1) The space F is (m-1)-connected for each $m{\leq}dim$ B. (H2) There is a relative CW-complex (Y, X) such that $B=Y{\times}I$ and $A=(X{\times}I)$ ${\cap}(Y{\times}O)$, where I=[0, 1]. (For proof see p.21 [2]).

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KINEMATIC STRUCTURES OF CERTAIN LOOPS

  • Im, Bok-Hee
    • Communications of the Korean Mathematical Society
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    • v.12 no.3
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    • pp.543-551
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    • 1997
  • In this paper, we call a loop F kinematic if for $a, b \in F\{0}$, the following two conditions are valid : (i) the centralizer Z(a) of a is a commutative group under the induced operation from the loop F, and (ii) Z(a) = Z(b) or $Z(a) \cap Z(b) = {0}$, where 0 is the identity of F. Some example of kinematic loops are given.

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THE LEBESGUE DELTA INTEGRAL

  • Park, Jae Myung;Lee, Deok Ho;Yoon, Ju Han;Lim, Jong Tae
    • Journal of the Chungcheong Mathematical Society
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    • v.27 no.3
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    • pp.489-494
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    • 2014
  • In this paper, we define the extension $f^*:[a,b]{\rightarrow}\mathbb{R}$ of a function $f:[a,b]_{\mathbb{T}}{\rightarrow}\mathbb{R}$ for a time scale $\mathbb{T}$ and investigate the properties of the Lebesgue delta integral of f on $[a,b]_{\mathbb{T}}$ by using the function $f^*$.

Comparative analysis of running-type brassieres on the market (런닝형 브래지어의 비교분석)

  • Park, Do Yoon;Jung, Heh Soon;Na, Mi Hyang
    • The Research Journal of the Costume Culture
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    • v.20 no.5
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    • pp.621-634
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    • 2012
  • With comparative analysis of running-type brassieres on the market about suitability for middle-aged women, this study is to provide basic data of running-type brassieres' pattern which is highly suitable. In the pursuit of this purpose, this study carried out wearing experiment targeting 3 middle-aged women with normal body shape which is 85B of chest circumference. The result is as follows. The significant difference was recognized in the breast. The products were rated in order of C>A>B>D, E, F, G>H by the observers, and C>B>D>A>F>E=G>H by the subjects. The significant difference was recognized in the armhole area. The products were ranked in the order of C, D>B, G, E>A, F, H by the observes and D, C>B>G>F>A>H by the subjects. The significant difference was also recognized in 6 categories(14~19) for the torso. According to the overall average values, the products were rated in the order of B, C, D>A, D, F, G by the observers and B=D>C>H>F>G>A>E by the subjects. The overall evaluation exhibited the significant difference. The products were ranked in the order of D>C>E>B>H>A>G>F by both observers and subjects. Therefore it was confirmed that the product D was appropriate in general. To conclude of comparative analysis about 8 kinds of running-type brassieres on the market, the average values of the product C and D in all categories had high marks for each category. The product C was comfortable and capable of covering the breast well, however, a gap was formed in outside-up part. The product D, made from lacy fabric, was very comfortable and well-fitted but there was inconsistency between the mold-cup and the breast shape.

LIPSCHITZ TYPE INEQUALITY IN WEIGHTED BLOCH SPACE Bq

  • Park, Ki-Seong
    • Journal of the Korean Mathematical Society
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    • v.39 no.2
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    • pp.277-287
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    • 2002
  • Let B be the open unit ball with center 0 in the complex space $C^n$. For each q>0, B$_{q}$ consists of holomorphic functions f : B longrightarrow C which satisfy sup z $\in$ B $(1-\parallel z \parallel^2)^q\parallel\nabla f(z)\parallel < \infty$ In this paper, we will show that functions in weighted Bloch spaces $B_{q}$ (0 < q < 1) satifies the following Lipschitz type result for Bergman metric $\beta$: |f(z)-f($\omega$)|< $C\beta$(z, $\omega$) for some constant C.