• Title/Summary/Keyword: F&B Event

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Rapid and Unequivocal Identification Method for Event-specific Detection of Transgene Zygosity in Genetically Modified Chili Pepper

  • Kang, Seung-Won;Lee, Chul-Hee;Seo, Sang-Gyu;Han, Bal-Kum;Choi, Hyung-Seok;Kim, Sun-Hyung;Harn, Chee-Hark;Lee, Gung-Pyo
    • Horticultural Science & Technology
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    • v.29 no.2
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    • pp.123-129
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    • 2011
  • To identify unintended vertical gene-transfer rates from the developed transgenic plants, rapid and unequivocal techniques are needed to identify event-specific markers based on flanking sequences around the transgene and to distinguish zygosity such as homo- and hetero-zygosity. To facilitate evaluation of zygosity, a polymerase chain reaction technique was used to analyze a transgenic pepper line B20 (homozygote), P915 wild type (null zygote), and their F1 hybrids, which were used as transgene contaminated plants. First, we sequenced the 3'-flanking region of the T-DNA (1,277 bp) in the transgenic pepper event B20. Based on sequence information for the 3'- and 5'-flanking region of T-DNA provided in a previous study, a primer pair was designed to amplify full length T-DNA in B20. We successfully amplified the full length T-DNA containing 986 bp from the flanking regions of B20. In addition, a 1,040 bp PCR product, which was where the T-DNA was inserted, was amplified from P915. Finally, both full length T-DNA and the 1,040 bp fragment were simultaneously amplified in the F1 hybrids; P915 ${\times}$ B20, Pungchon ${\times}$ B20, Gumtap ${\times}$ B20. In the present study, we were able to identify zygosity among homozygous transgenic event B20, its wild type P915, and hemizygous F1 hybrids. Therefore, this novel zygosity identification technique, which is based on PCR, can be effectively used to examine gene flow for transgenic pepper event B20.

Rapid Depressurization Capability of Monobloc Sebim Valves for KNGR Total Loss of Feedwater Event

  • Kwon, Young-Min;Lim, Hong-Sik;Song, Jin-Ho
    • Proceedings of the Korean Nuclear Society Conference
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    • 1996.11a
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    • pp.389-394
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    • 1996
  • The conceptual design of Korea Next Generation Reactor (KNGR), which is 3914 MWt PWR, includes the safety depressurization system (SDS) to comply with U.S. NRC's severe accident policy. In this analysis, it is assumed that three Monobloc Sebim valves are adopted for the SDS bleed valves of KNGR. The characteristic of Monobloc Sebim are modeled in the CE-FLASH-4AS/REM code for this analysis. The various feed and bleed (F&B) procedures with Sebim valves are investigated for total loss of feedwater (TLOFW) event. It is found that if operators open two out of three Sebim valves in conjunction with four HPSI pumps before hot leg temperature reaches saturation condition, the decay heat removal and core inventory make-up function can be successfully accomplished. Therefore, this F&B procedure can be used for mitigating the TLOFW event of the KNGR. This result also demonstrates the feasibility of adopting the Monobloc Sebim valves for the SDS of KNGR.

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Normal fuzzy probability for generalized triangular fuzzy sets (일반화된 삼각퍼지집합에 대한 정규퍼지확률)

  • Kang, Chul;Yun, Yong-Sik
    • Journal of the Korean Institute of Intelligent Systems
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    • v.22 no.2
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    • pp.212-217
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    • 2012
  • A fuzzy set $A$ defined on a probability space ${\Omega}$, $\mathfrak{F}$, $P$ is called a fuzzy event. Zadeh defines the probability of the fuzzy event $A$ using the probability $P$. We define the generalized triangular fuzzy set and apply the extended algebraic operations to these fuzzy sets. A generalized triangular fuzzy set is symmetric and may not have value 1. For two generalized triangular fuzzy sets $A$ and $B$, $A(+)B$ and $A(-)B$ become generalized trapezoidal fuzzy sets, but $A({\cdot})B$ and $A(/)B$ need not to be a generalized triangular fuzzy set or a generalized trapezoidal fuzzy set. We define the normal fuzzy probability on $\mathbb{R}$ using the normal distribution. And we calculate the normal fuzzy probability for generalized triangular fuzzy sets.

Analysis of Total Loss of Feedwater Event for the Determination of Safety Depressurization Bleed Capacity (안전감압계통의 방출유량을 결정하기 위한 완전급수상실사고 해석)

  • Kwon, Young-Min;Song, Jin-Ho;Ro, Tae-Sun
    • Nuclear Engineering and Technology
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    • v.27 no.4
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    • pp.470-482
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    • 1995
  • The Ulchin 3&4, which are 2825 MWt PWRs, adopted Safety Depressurization System (SDS) to mitigate the beyond design basis event of Total Less of Feedwater(TLOFW). In this study the results and methodology of the analyses for the determination of SDS bleed capacity are discussed. The SDS design bleed capacity has been determined from the CEFLASH-4AS/REM simulation according to the following design criteria : 1) Each SDS flow path, in conjunction with one of two High Pressure Safety Injection (HPSI) pumps, is designed to have a sufficient capacity to prevent core uncovery if one SDS path is opened simultaneously with the opening of the Pressurizer Safety Valves (PSVs). 2) Both SDS bleed paths are designed to have sufficient total capacity with both HPSI pumps operating to prevent core uncovery if the Feed and Bleed (F&B) initiation is delayed up to thirty minutes from the time of the PSVs lift. To verify the results of CEFLASH-4AS/REM simulation a comparative analysis kas also been per-formed by more sophisticated computer code, RELAP5/MOD3. The TLOFW event without operator recovery and TLOFW event with F&B are analyzed. The predictions by the CEFLASH-4AS/REM of the transient too phase system behavior are in good qualitative and quantitative agreement with those by the RELAP5/MOD3 simulation. Both of the results of analyses by CEFLASH-4AS/REM and RELAP5/MOD3 have demonstrated that decay heat removal and core inventory make-up can be successfully accomplished by F&B operation during now event for the Ulchin 3&4.

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On the origin of exponential growth in induced earthquakes in Groningen

  • van Putten, Maurice H.P.M.;van Putten, Anton F.P.;van Putten, Michael J.A.M.
    • Earthquakes and Structures
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    • v.11 no.5
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    • pp.861-871
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    • 2016
  • The Groningen gas field shows exponential growth in earthquake event counts around a magnitude M1 with a doubling time of 6-9 years since 2001. This behavior is identified with dimensionless curvature in land subsidence, which has been evolving at a constant rate over the last few decades essentially uncorrelated to gas production. We demonstrate our mechanism by a tabletop crack formation experiment. The observed skewed distribution of event magnitudes is matched by that of maxima of event clusters with a normal distribution. It predicts about one event < M5 per day in 2025, pointing to increasing stress to human living conditions.

Application of ETA(Event Tree Analysis) to the Performance-Based Design of fire protection (성능위주설계를 위한 ETA 기법 활용 고찰)

  • Kim, H.B.;Lee, S.K.;Song, D.W.;Kim, K.S.;Kim, J.H.
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2011.11a
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    • pp.454-457
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    • 2011
  • 본 연구에서는 소방시설의 성능위주의설계를 수행하기 위하여 ETA(Event Tree Analysis) 기법을 적용하는 방안을 고찰하였다. ETA기법에서는 화재시나리오를 사건(Event)의 인과관계로 된 각 단계의 사건으로 구성한다. 본 연구에서는 ETA에서 구성된 시나리오에 따른 심각도를 화재시뮬레이션과 피난 시뮬레이션을 통한 수행으로 사망자수를 도출하는 방안을 적용하였고, 각 시나리오의 빈도(확률)은 FTA(Fault Tree Analysis) 기법을 적용하여 분기 확률을 도출하도록 하였다. ETA에서 도출한 사망자수와 빈도를 이용하여 F-N 커브를 작성하여 위험도를 평가하여 소방설계의 보완 및 대책을 수립하는 방안을 제시하였다.

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Feasibility of Long Term Feed and Bleed Operation For Total Loss of Feedwater Event

  • Kwon, Young-Min;Song, Jin-Ho
    • Nuclear Engineering and Technology
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    • v.28 no.3
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    • pp.257-264
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    • 1996
  • The conventional Equipment Environment Qualification (EEQ) envelope is developed based on the containment responses during the design basis events. The Safety Depressurization System (SDS) design without In-containment Refueling Water Storage Tank (IRWST) adopted in the Ulchin 3&4 challenges the conventional EEQ envelope during long term Feed and Bleed (F&B) operation due to the direct discharge of high mass and energy into the containment. Therefore, it is necessary to confirm that the containment pressure and temperature history during the long term F&B operation does not violate the conventional EEQ envelope. However, this subject has never been quantitatively assessed before. To investigate the success path of long term F&B operation this paper analyzes the thermal hydraulic response of the containment and Reactor Coolant System (RCS) until the completion of depressurization and cooldown of RCS into Shutdown Cooling System (SCS) entry condition. It is found that the SCS entry condition can be reached within 6 hours without violating the EEQ curve by proper operation of SDS valves, High Pressure Safety Injection (HPSI) pumps and active Containment Heat Removal System (CHRS). The suggested strategy not only demonstrates the feasibility of long term F&B operation but also can be utilized in the preparation of Emergency Procedure Guidelines (EPGs)

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Experimental Study of Second Harmonic Ultrasound imaging with a Weighted Chirp Signal (가중 쳐프 신호를 사용한 초음파 고조파 영상 기법의 실험적 고찰)

  • 김동열;이종철;송태경
    • Proceedings of the IEEK Conference
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    • 2001.06d
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    • pp.151-154
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    • 2001
  • In this Paper, a new harmonic imaging technique is proposed and evaluated experimentally. In the proposed method, a weighted chin signal with a hanning window is transmitted. The RF samples obtained on each array element are individually compressed by correlating with the reference signal defined as the 2nd harmonic (2f0) component of a transmitted chirp signal generated in a square-law system. The proposed method uses the compressed 2f0 component to form an image, for which the crosscorrelation term with f0 component should be suppressed below at least -60dB. After experiment, the 6dB pulse width and peak sidelobe level of the compressed 2f0 component were 0.7us and -60dB, respectively. This result shows that the proposed method can successfully eliminate the f0 component with a single transmit-receive event and therefore is more efficient than the conventional pulse inversion (PI) method in terms of frame rate. We also observed that the 2nd harmonic compont starts to decrease for source pressure higher than 210kPa in water, which implies that SNR of the 2nd harmonic imaging using short pulses cnanot be incresed beyond a certain limit.

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Active Control of Flow Noise Sources in Turbulent Boundary Layer on a Flat-Plate Using Piezoelectric Bimorph Film

  • Song, Woo-Seog;Lee, Seung-Bae;Shin, Dong-Shin;Na, Yang
    • Journal of Mechanical Science and Technology
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    • v.20 no.11
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    • pp.1993-2001
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    • 2006
  • The piezoelectric bimorph film, which, as an actuator, can generate more effective displacement than the usual PVDF film, is used to control the turbulent boundary-layer flow. The change of wall pressures inside the turbulent boundary layer is observed by using the multi-channel microphone array flush-mounted on the surface when actuation at the non-dimensional frequency $f_b^+$:=0.008 and 0.028 is applied to the turbulent boundary layer. The wall pressure characteristics by the actuation to produce local displacement are more dominantly influenced by the size of the actuator module than the actuation frequency. The movement of large-scale turbulent structures to the upper layer is found to be the main mechanism of the reduction in the wall- pressure energy spectrum when the 700$700{\nu}/u_{\tau}$-long bimorph film is periodically actuated at the non- dimensional frequency $f_b^+$:=0.008 and 0.028. The biomorph actuator is triggered with the time delay for the active forcing at a single frequency when a 1/8' pressure-type, pin-holed microphone sensor detects the large-amplitude pressure event by the turbulent spot. The wall-pressure energy in the late-transitional boundary layer is partially reduced near the convection wavenumber by the open-loop control based on the large amplitude event.

Brain-Computer Interface based on Changes of EEG on Broca's Area (Broca 영역에서의 뇌파 변화에 기반한 뇌-컴퓨터 인터페이스)

  • Yeom, Hong-Gi;Jang, In-Hun;Sim, Kwee-Bo
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.1
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    • pp.122-127
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    • 2009
  • In this paper, we measured EEG signals on frontal and Broca's area when subjects imagine to speak A or B or C or D. These signals were analyzed by Event-Related Spectral Perturbation (ERSP), Inter-Trial Coherence (ITC) and Event Related Potential (ERP) methods. As a result, high coherences were showed at 1$\sim$13Hz during 0$\sim$300ms after the stimuli of each character and P300 was seen clearly and there are several differences between the ERP results. However, unlike the motivation of this study to classify the characters, it is impossible that we can classify each intention or each character cause these differences. Nevertheless, this paper suggest an application system using this results so BCI can provide various services.