• 제목/요약/키워드: F&B Event

검색결과 37건 처리시간 0.029초

Rapid and Unequivocal Identification Method for Event-specific Detection of Transgene Zygosity in Genetically Modified Chili Pepper

  • Kang, Seung-Won;Lee, Chul-Hee;Seo, Sang-Gyu;Han, Bal-Kum;Choi, Hyung-Seok;Kim, Sun-Hyung;Harn, Chee-Hark;Lee, Gung-Pyo
    • 원예과학기술지
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    • 제29권2호
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    • pp.123-129
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    • 2011
  • To identify unintended vertical gene-transfer rates from the developed transgenic plants, rapid and unequivocal techniques are needed to identify event-specific markers based on flanking sequences around the transgene and to distinguish zygosity such as homo- and hetero-zygosity. To facilitate evaluation of zygosity, a polymerase chain reaction technique was used to analyze a transgenic pepper line B20 (homozygote), P915 wild type (null zygote), and their F1 hybrids, which were used as transgene contaminated plants. First, we sequenced the 3'-flanking region of the T-DNA (1,277 bp) in the transgenic pepper event B20. Based on sequence information for the 3'- and 5'-flanking region of T-DNA provided in a previous study, a primer pair was designed to amplify full length T-DNA in B20. We successfully amplified the full length T-DNA containing 986 bp from the flanking regions of B20. In addition, a 1,040 bp PCR product, which was where the T-DNA was inserted, was amplified from P915. Finally, both full length T-DNA and the 1,040 bp fragment were simultaneously amplified in the F1 hybrids; P915 ${\times}$ B20, Pungchon ${\times}$ B20, Gumtap ${\times}$ B20. In the present study, we were able to identify zygosity among homozygous transgenic event B20, its wild type P915, and hemizygous F1 hybrids. Therefore, this novel zygosity identification technique, which is based on PCR, can be effectively used to examine gene flow for transgenic pepper event B20.

Rapid Depressurization Capability of Monobloc Sebim Valves for KNGR Total Loss of Feedwater Event

  • Kwon, Young-Min;Lim, Hong-Sik;Song, Jin-Ho
    • 한국원자력학회:학술대회논문집
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    • 한국원자력학회 1996년도 추계학술발표회논문집(1)
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    • pp.389-394
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    • 1996
  • The conceptual design of Korea Next Generation Reactor (KNGR), which is 3914 MWt PWR, includes the safety depressurization system (SDS) to comply with U.S. NRC's severe accident policy. In this analysis, it is assumed that three Monobloc Sebim valves are adopted for the SDS bleed valves of KNGR. The characteristic of Monobloc Sebim are modeled in the CE-FLASH-4AS/REM code for this analysis. The various feed and bleed (F&B) procedures with Sebim valves are investigated for total loss of feedwater (TLOFW) event. It is found that if operators open two out of three Sebim valves in conjunction with four HPSI pumps before hot leg temperature reaches saturation condition, the decay heat removal and core inventory make-up function can be successfully accomplished. Therefore, this F&B procedure can be used for mitigating the TLOFW event of the KNGR. This result also demonstrates the feasibility of adopting the Monobloc Sebim valves for the SDS of KNGR.

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일반화된 삼각퍼지집합에 대한 정규퍼지확률 (Normal fuzzy probability for generalized triangular fuzzy sets)

  • 강철;윤용식
    • 한국지능시스템학회논문지
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    • 제22권2호
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    • pp.212-217
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    • 2012
  • 확률공간 (${\Omega}$, $\mathfrak{F}$, $P$) 위에 정의된 퍼지집합을 퍼지이벤트라 한다. Zadeh는 확률 $P$를 이용하여 퍼지이벤트 $A$에 대한 확률을 정의하였다. 우리는 일반화된 삼각퍼지집합을 정의하고 거기에 확장된 대수적 작용소를 적용하였다. 일반화된 삼각퍼지집합은 대칭적이지만 함숫값으로 1을 갖지 않을 수 있다. 두 개의 일반화된 삼각퍼지집합 $A$$B$에 대하여 $A(+)B$$A(-)B$는 일반화된 사다리꼴퍼지집합이 되었지만, $A({\cdot})B$$A(/)B$는 일반화된 삼각퍼지집합도 되지 않았고 일반화된 사다리꼴퍼지집합도 되지 않았다. 그리고 정규분포를 이용하여 $\mathbb{R}$위에서 정규퍼지확률을 정의하였다. 그리고 일반화된 삼각퍼지집합에 대한 정규퍼지확률을 계산하였다.

안전감압계통의 방출유량을 결정하기 위한 완전급수상실사고 해석 (Analysis of Total Loss of Feedwater Event for the Determination of Safety Depressurization Bleed Capacity)

  • Kwon, Young-Min;Song, Jin-Ho;Ro, Tae-Sun
    • Nuclear Engineering and Technology
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    • 제27권4호
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    • pp.470-482
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    • 1995
  • 2025 MWt 가압경수로인 울진 3, 4호기에는 설계기준초과사고인 완전급수상실사고를 완화하기 위하여 안전감압계통이 채택되었다. 본 논문은 울진 3, 4호기의 안전감압계통의 방출유량을 결정하기 위한 해석방법 및 결과에 대하여 논의하였다. 안전감압계통의 방출용량을 다음과 같은 두가지의 설계요건에 따라 결정하였다 : 1) 두 개의 고압안전주입펌프 중 하나의 펌프만이 작동하고 운전원이 안전감압계통의 한 계열의 감압경로를 가압기안전밸브가 열리자마자 개방하였을 경우 노심노출을 방지하여야 한다 2) 두 개의 고압안전주입펌프가 모두 작동하고 두 계열의 안전감압경로를 가압기안전밸브가 열린 후 30분 뒤에 개방하였을 경우 노심노출을 방지하여야 한다. CEFLASH-4AS/REM 전산코드의 모델 및 해석 결과의 타당성을 검토하기 위하여 REL-AP5/MOD3를 이용한 해석을 수행하였다. 운전원의 복구과정이 없을 경우와 운전원이 충전 및 유출운전에 의해 사고를 완화하는 경우의 완전급수상실사고 경위에 대해 수치모사를 수행하였다. 두 사고 경 위에 대해 CEFLASH-4AS/REM에 의해 예측된 원자로계통의 주요 열수력학적 거동이 RELAP5 /MOD3에 의한 결과와 정성·정량적으로 잘 일치하는 것을 알 수 있었다. 결론적으로 울진 3, 4호기에 대해 완전급수상실사고시 안전감압계통을 이용한 충전 및 유출운전에 의해 잔열제거 및 일차계통냉각재 재고량 유지가 성공적으로 이루어짐을 수치모사를 통해 확인 할 수 있었다.

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On the origin of exponential growth in induced earthquakes in Groningen

  • van Putten, Maurice H.P.M.;van Putten, Anton F.P.;van Putten, Michael J.A.M.
    • Earthquakes and Structures
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    • 제11권5호
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    • pp.861-871
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    • 2016
  • The Groningen gas field shows exponential growth in earthquake event counts around a magnitude M1 with a doubling time of 6-9 years since 2001. This behavior is identified with dimensionless curvature in land subsidence, which has been evolving at a constant rate over the last few decades essentially uncorrelated to gas production. We demonstrate our mechanism by a tabletop crack formation experiment. The observed skewed distribution of event magnitudes is matched by that of maxima of event clusters with a normal distribution. It predicts about one event < M5 per day in 2025, pointing to increasing stress to human living conditions.

성능위주설계를 위한 ETA 기법 활용 고찰 (Application of ETA(Event Tree Analysis) to the Performance-Based Design of fire protection)

  • 김학범;이수경;송동우;김기성;김종훈
    • 한국화재소방학회:학술대회논문집
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    • 한국화재소방학회 2011년도 추계학술논문발표회 논문집
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    • pp.454-457
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    • 2011
  • 본 연구에서는 소방시설의 성능위주의설계를 수행하기 위하여 ETA(Event Tree Analysis) 기법을 적용하는 방안을 고찰하였다. ETA기법에서는 화재시나리오를 사건(Event)의 인과관계로 된 각 단계의 사건으로 구성한다. 본 연구에서는 ETA에서 구성된 시나리오에 따른 심각도를 화재시뮬레이션과 피난 시뮬레이션을 통한 수행으로 사망자수를 도출하는 방안을 적용하였고, 각 시나리오의 빈도(확률)은 FTA(Fault Tree Analysis) 기법을 적용하여 분기 확률을 도출하도록 하였다. ETA에서 도출한 사망자수와 빈도를 이용하여 F-N 커브를 작성하여 위험도를 평가하여 소방설계의 보완 및 대책을 수립하는 방안을 제시하였다.

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Feasibility of Long Term Feed and Bleed Operation For Total Loss of Feedwater Event

  • Kwon, Young-Min;Song, Jin-Ho
    • Nuclear Engineering and Technology
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    • 제28권3호
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    • pp.257-264
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    • 1996
  • The conventional Equipment Environment Qualification (EEQ) envelope is developed based on the containment responses during the design basis events. The Safety Depressurization System (SDS) design without In-containment Refueling Water Storage Tank (IRWST) adopted in the Ulchin 3&4 challenges the conventional EEQ envelope during long term Feed and Bleed (F&B) operation due to the direct discharge of high mass and energy into the containment. Therefore, it is necessary to confirm that the containment pressure and temperature history during the long term F&B operation does not violate the conventional EEQ envelope. However, this subject has never been quantitatively assessed before. To investigate the success path of long term F&B operation this paper analyzes the thermal hydraulic response of the containment and Reactor Coolant System (RCS) until the completion of depressurization and cooldown of RCS into Shutdown Cooling System (SCS) entry condition. It is found that the SCS entry condition can be reached within 6 hours without violating the EEQ curve by proper operation of SDS valves, High Pressure Safety Injection (HPSI) pumps and active Containment Heat Removal System (CHRS). The suggested strategy not only demonstrates the feasibility of long term F&B operation but also can be utilized in the preparation of Emergency Procedure Guidelines (EPGs)

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가중 쳐프 신호를 사용한 초음파 고조파 영상 기법의 실험적 고찰 (Experimental Study of Second Harmonic Ultrasound imaging with a Weighted Chirp Signal)

  • 김동열;이종철;송태경
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2001년도 하계종합학술대회 논문집(4)
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    • pp.151-154
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    • 2001
  • In this Paper, a new harmonic imaging technique is proposed and evaluated experimentally. In the proposed method, a weighted chin signal with a hanning window is transmitted. The RF samples obtained on each array element are individually compressed by correlating with the reference signal defined as the 2nd harmonic (2f0) component of a transmitted chirp signal generated in a square-law system. The proposed method uses the compressed 2f0 component to form an image, for which the crosscorrelation term with f0 component should be suppressed below at least -60dB. After experiment, the 6dB pulse width and peak sidelobe level of the compressed 2f0 component were 0.7us and -60dB, respectively. This result shows that the proposed method can successfully eliminate the f0 component with a single transmit-receive event and therefore is more efficient than the conventional pulse inversion (PI) method in terms of frame rate. We also observed that the 2nd harmonic compont starts to decrease for source pressure higher than 210kPa in water, which implies that SNR of the 2nd harmonic imaging using short pulses cnanot be incresed beyond a certain limit.

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Active Control of Flow Noise Sources in Turbulent Boundary Layer on a Flat-Plate Using Piezoelectric Bimorph Film

  • Song, Woo-Seog;Lee, Seung-Bae;Shin, Dong-Shin;Na, Yang
    • Journal of Mechanical Science and Technology
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    • 제20권11호
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    • pp.1993-2001
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    • 2006
  • The piezoelectric bimorph film, which, as an actuator, can generate more effective displacement than the usual PVDF film, is used to control the turbulent boundary-layer flow. The change of wall pressures inside the turbulent boundary layer is observed by using the multi-channel microphone array flush-mounted on the surface when actuation at the non-dimensional frequency $f_b^+$:=0.008 and 0.028 is applied to the turbulent boundary layer. The wall pressure characteristics by the actuation to produce local displacement are more dominantly influenced by the size of the actuator module than the actuation frequency. The movement of large-scale turbulent structures to the upper layer is found to be the main mechanism of the reduction in the wall- pressure energy spectrum when the 700$700{\nu}/u_{\tau}$-long bimorph film is periodically actuated at the non- dimensional frequency $f_b^+$:=0.008 and 0.028. The biomorph actuator is triggered with the time delay for the active forcing at a single frequency when a 1/8' pressure-type, pin-holed microphone sensor detects the large-amplitude pressure event by the turbulent spot. The wall-pressure energy in the late-transitional boundary layer is partially reduced near the convection wavenumber by the open-loop control based on the large amplitude event.

Broca 영역에서의 뇌파 변화에 기반한 뇌-컴퓨터 인터페이스 (Brain-Computer Interface based on Changes of EEG on Broca's Area)

  • 염홍기;장인훈;심귀보
    • 한국지능시스템학회논문지
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    • 제19권1호
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    • pp.122-127
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    • 2009
  • 본 논문에서는 피험자가 A, B, C, D 글자를 말하는 상상을 할 때 사고중추에서와 Broca's area 에서 EEG 신호를 측정하였으며 이 신호를 Event-Related Spectral Perturbation (ERSP), Inter-Trial Coherence (ITC) 그리고 Event Related Potential (ERP) 방법을 통해 분석하여 보았다. 그 결과 F7, FT7 영역의 뇌파에서 각 문자를 보여주는 자극 제시 후 0$\sim$300ms 동안의 1$\sim$13Hz에서 높은 coherence를 보였으며, P300 이 뚜렷하게 나타나는 것을 확인할 수 있었다. 하지만 ERP를 통해 분석해본 결과 각 글자에 대한 차이를 구분하고자 하였던 처음 연구의 동기와 달리 각 글자를 말할 때 ERP가 약간의 차이를 보이기는 하였으나 각 문자에 대한 차이라거나 이 차이를 통해 문자를 구별할 수 있다고 하기는 어려웠다. 하지만 본 논문에서는 이 실험결과를 통해 기존에 운동관련 뇌 영역에 국한되어 있던 BCI 연구의 한계를 극복하고 보다 다양한 서비스를 제공할 수 있는 응용 시스템을 제안하였다.