• Title/Summary/Keyword: Expression characteristics

Search Result 2,607, Processing Time 0.033 seconds

Characteristics of concrete intensity using high early strength AE water reducing agent (조강형 AE감수제를 사용한 콘크리트의 강도발현 특성)

  • Kim, Jung-Tai;Kim, Seung-Han;Jang, Seck-Soo;Jung, Yong-Wook;Yeo, In-Dong
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 2008.04a
    • /
    • pp.793-796
    • /
    • 2008
  • Recently early strength concrete has been required for economical assurance and the prevention of frost damage in winter through air reduction in construction of concrete structures. This study presented the optimum condition revealing compressive strength 5MPa which has the possibility of removal of form in 24 hours, and researched the changes of unit weight of cement types of high early strength AE water reducing agents, characteristic of compressive strength expression as cure temperature conditions and slump or airspace. Test results showed at $15^{\circ}C$ with compressive strength of 5MPa that premature removal of form was possible in case of using highly early strength PC water reducing agent with unit weight of cement 360 ; 22hours faster than 10, unit weight 360 ; 20hours faster than 7, unit weight 390 ; 18 hours faster than 4 comparing with OP water reducing agent. And at $5^{\circ}C$ in case of using highly early strength PC water reducing agent with unit weight of cement 330 ; 32hours faster than 10, unit weight 360 ; 30hours faster than 7, unit weight390 ; 27hours faster than 4 comparing with OP water reducing agent. Therefore as the temperature rises $10^{\circ}C$, compressive strength of 5MPa reaching hour shortens 10 hours.

  • PDF

Pattern-based Signature Generation for Identification of HTTP Applications (HTTP 응용들의 식별을 위한 패턴 기반의 시그니쳐 생성)

  • Jin, Chang-Gyu;Choi, Mi-Jung
    • Journal of Information Technology and Architecture
    • /
    • v.10 no.1
    • /
    • pp.101-111
    • /
    • 2013
  • Internet traffic volume has been increasing rapidly due to popularization of various smart devices and Internet development. In particular, HTTP-based traffic volume of smart devices is increasing rapidly in addition to desktop traffic volume. The increased mobile traffic can cause serious problems such as network overload, web security, and QoS. In order to solve these problems of the Internet overload and security, it is necessary to accurately detect applications. Traditionally, well-known port based method is utilized in traffic classification. However, this method shows low accuracy since P2P applications exploit a TCP/80 port, which is used for the HTTP protocol; to avoid firewall or IDS. Signature-based method is proposed to solve the lower accuracy problem. This method shows higher analysis rate but it has overhead of signature generation. Also, previous signature-based study only analyzes applications in HTTP protocol-level not application-level. That is, it is difficult to identify application name. Therefore, previous study only performs protocol-level analysis. In this paper, we propose a signature generation method to classify HTTP-based traffics in application-level using the characteristics of typical semi HTTP header. By applying our proposed method to campus network traffic, we validate feasibility of our method.

Nucleotide Sequences of β-lactoglobulin Gene 5'Flanking Region in Korean Native Goat (한국재래산양 β-lactoglobulin 유전자 5'flanking 영역의 염기서열 분석)

  • Ryoo, Seung-Heui;Han, Sung-Wook;Seo, Kil-Woong;Sang, Byung-Chan
    • Korean Journal of Agricultural Science
    • /
    • v.28 no.2
    • /
    • pp.78-84
    • /
    • 2001
  • This study was analyzed by PCR technique with specific primer in order to investigate the characteristics of ${\beta}$-lactoglobulin (${\beta}$-LG) gene 5'flanking region in Korean native goat. This work confirmed amplified product of 1,077 bp fragments obtained from the amplification of ${\beta}$-LG promoter from genomic DNA using PCR in Korean native goat. The nucleotide sequence of ${\beta}$-LG gene 5'flanking region in Korean native goat as compared with that of sheep ${\beta}$-LG were different at 46 base of 897 nucleotides, and showed high homology as about 94.9% each breed. Especially we confirmed that the difference of nucleotide sequences between Korean native goat and sheep were consisted of $T{\rightarrow}C$ substitution and $C{\rightarrow}T$ substitution reversely. As a consequences, the sequences of ${\beta}$-LG gene 5'flanking region showed a high homology between Korean native goat and sheep. Furthermore we should be studied that relationships between the control of gene expression and nucleotide sequences of transcription factor in Korean native goat.

  • PDF

A Study of Field Application Process of Public Key Algorithm RSA Based on Mathematical Principles and Characteristics through a Diagnostic (수학원리와 특성 진단을 기반으로 한 공개키 RSA 알고리즘의 현장 적용 프로세스)

  • Noh, SiChoon;Song, EunJee;Moon, SongChul
    • Journal of Service Research and Studies
    • /
    • v.5 no.2
    • /
    • pp.71-81
    • /
    • 2015
  • The RSA public key encryption algorithm, a few, key generation, factoring, the Euler function, key setup, a joint expression law, the application process are serial indexes. The foundation of such algorithms are mathematical principles. The first concept from mathematics principle is applied from how to obtain a minority. It is to obtain a product of two very large prime numbers, but readily tracking station the original two prime number, the product are used in a very hard principles. If a very large prime numbers p and q to obtain, then the product is the two $n=p{\times}q$ easy station, a method for tracking the number of p and q from n synthesis and it is substantially impossible. The RSA encryption algorithm, the number of digits in order to implement the inverse calculation is difficult mathematical one-way function and uses the integer factorization problem of a large amount. Factoring the concept of the calculation of the mod is difficult to use in addition to the problem in the reverse direction. But the interests of the encryption algorithm implementation usually are focused on introducing the film the first time you use encryption algorithm but we have to know how to go through some process applied to the field work This study presents a field force applied encryption process scheme based on public key algorithms attribute diagnosis.

Effects of low temperature-adapted Saccharomyces cerevisiae Y297 strain and fermentation temperature on the quality characteristics of Yakju (저온 적응성 효모와 발효온도에 따른 약주의 품질특성 변화)

  • Seo, Dong-Jun;Yeo, Soo-Hwan;Mun, Ji-Young;Baek, Seong Yeol
    • Food Science and Preservation
    • /
    • v.23 no.5
    • /
    • pp.666-672
    • /
    • 2016
  • The objective of this study was to evaluate the effects of low temperature-adapted Saccharomyces cerevisiae Y297 and fermentation temperatures on the quality of Yakju brewed. Physicochemical properties of Yakju brewed were compared pH, total acidity, ethanol, free amino acid, organic acid contents, and volatile flavor compounds in S. cerevisiae Y297 with control treatment. Cooked non-glutinous rice and saccharogenic amylase in koji were mixed with ethanol-producing yeasts and then fermented at $15^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$ for 20 days. Yakju brewed using the Y297 treatment showed the highest ethanol yield (17.9%) at $20^{\circ}C$. Expression of heat shock protein (HSP) 104 was evaluated by immunoblotting as an indication of adaptation to low temperatures ($15^{\circ}C$); levels of the HSP104 protein were higher in the Y297 treatment than in the control. Organic acid analysis showed that the lactic acid content of Yakju brewed using the control was the highest at $25^{\circ}C$. Finally, free amino acid analysis showed that the Y297 treatment had a higher proportion of essential amino acids than the control. Overall, these results indicate that S. cerevisiae Y297 could be used as a suitable yeast for Yakju brewed under low temperature ($15^{\circ}C$) condition.

Expression of image contents based on property of digital signage - Focuses on the Digital Signage in Public Transport (디지털 사이니지의 특성에 따른 영상콘텐츠의 변화 -버스와 지하철 내 디지털 사이니지를 중심으로)

  • Kong, Soo-Kyung
    • Journal of Digital Contents Society
    • /
    • v.16 no.5
    • /
    • pp.783-793
    • /
    • 2015
  • TV display which existed only in house started to show up out of house around us and now it found naturally its place in everywhere like street, bus, subway and elevator. It is called digital signage which showed up through digitalization of sign, that is, sign board and bulletin board. The distinction of digital sign from existing signs is that the latter one should go through physical process like removal after installation every time its contents are changed but the former one can produce its various outputs flexibly once it is installed. Also existing sign may be static image or 2 or 3 pieces of image to express simple motion while digital sign can contain multi media contents luxurious in design and motion. This paper confined the range of contents in digital signage in bus and subway. It needs to analyze characteristics of mass transportation-people of use, consumer by place and time, accommodation environment for consumer etc and arrange planned contents along with time and place. Developments of dedicated contents suitable to those digital signages will harmonize with place and time and promote the realm of digital signage which provides variety of experience to consumer and with which communication is possible and which is distinctive. Furthermore we may expect the birth of smart signage as a new media, in which fun and art are combined.

A study of the didactic character of Huang Yunseok's sijo and it's implications for his poetic intentions (황윤석 시조의 교술적 성격과 작가 의식)

  • 전재강
    • Sijohaknonchong
    • /
    • v.19 no.1
    • /
    • pp.207-234
    • /
    • 2003
  • The purpose of this research paper is to study the characteristics of Huang Yunseok's sijo and it's implications for his poetic intentions. Huang Yunseok's sijo is didactic in its presentation of facts and ethical lessons. The two main facts it presents are royal favors and his clan's pride. Royal favors concern. for example, encouragement of the king, the king's birthday and deathday, and securing a government position by the king's order. His clan's pride concerns, for example, the geographically wonderful place of Huang Yunseok's ancestor's tomb, his ancestor's great conduct, his clan's beautiful tradition, his birth place, and his birth dream. The main content of the ethical lessons concerns cultivation of the human mind and practicing Confucian ethics. Examples of the former describe the purpose of Confucian study, the ethical doctrine that human being's inborn nature is good, the character of the human mind, and the method of cultivating the human mind. Examples of the latter describe the moral rules to govern the Five Human Relations, separation between husband and wife, and discrimination between males and females. The poetic intention of Huang Yunseok's sip consists of political and economic self-promotion and theoretical devotion to Confucian ideology. Proud of his educated, high Confucian clan, he tries to get self-promotion politically and economically by vowing loyalty to the king. He implies devotion to the Confucian ideology through his description of the practice of the Confucian moral rules, the clinging to traditional Neo-Confucianism, and his insistence on the Neo-Confucian theory that all human beings and animals are the same in their original nature. In conclusion, the didactic character of Huang Yunseok's sip stems from his intention to promote himself politically and economically and his theoretical devotion to Confucian ideology. The way of indicative expression originates from the didactic character of Huang Yunseok's sijo.

  • PDF

Phosphorylation Properties of Recombinant OsCPK11, a Calcium-dependent Protein Kinase from Rice (벼의 칼슘-의존적 단백질 카이네즈인 재조합 OsCPK11의 인산화 특성)

  • Cho, Il-Sang;Lee, Su-Hee;Park, Chung-Mo;Kim, Sung-Ha
    • Journal of Life Science
    • /
    • v.27 no.12
    • /
    • pp.1393-1402
    • /
    • 2017
  • In plants, calcium ($Ca^{2+}$)-dependent protein kinases (CDPKs) are important sensors of $Ca^{2+}$ signals. Previous research demonstrated the expression of the OsCPK11 gene in various tissues at the transcription level, but its developmental and biochemical functions at the protein level were not determined. This study was aimed to identify biochemical characteristics of OsCPK11. GST- OsCPK11 was expressed in E. coli and used for an in vitro kinase assay. Biochemical analyses identified OsCPK11 as a CDPK. OsCPK11 autophosphorylated itself and transphosphorylated histone III-s and MBP as substrates in the presence of $Ca^{2+}$. The activity of the recombinant OsCPK11 was influenced by $Mg^{2+}$, with optimum activity detected at pH 7.0-7.5. OsCPK11 activity was not affected by $Mg^{2+}$, $Mn^{2+}$, or $Na^+$ in the presence of a high level of $Ca^{2+}$. Autophosphorylation of OsCPK11 decreased $Ca^{2+}$ sensitivity of OsCPK11. An anti-OsCPK11 rabbit antibody recognized 95.5 kD of GST-OsCPK11, as shown by an immunoblot analysis. These results shed light on the function of OsCPK11 in $Ca^{2+}$-mediated signaling in rice.

Characterization of Primary Epithelial Cells Derived from Human Salivary Gland Contributing to in vivo Formation of Acini-like Structures

  • Nam, Hyun;Kim, Ji-Hye;Hwang, Ji-Yoon;Kim, Gee-Hye;Kim, Jae-Won;Jang, Mi;Lee, Jong-Ho;Park, Kyungpyo;Lee, Gene
    • Molecules and Cells
    • /
    • v.41 no.6
    • /
    • pp.515-522
    • /
    • 2018
  • Patients with head and neck cancer are treated with therapeutic irradiation, which can result in irreversible salivary gland dysfunction. Because there is no complete cure for such patients, stem cell therapy is an emerging alternative for functional restoration of salivary glands. In this study, we investigated in vitro characteristics of primarily isolated epithelial cells from human salivary gland (Epi-SGs) and in vivo formation of acini-like structures by Epi-SGs. Primarily isolated Epi-SGs showed typical epithelial cell-like morphology and expressed E-cadherin but not N-cadherin. Epi-SGs expressed epithelial stem cell (EpiSC) and embryonic stem cell (ESC) markers. During long-term culture, the expression of EpiSC and ESC markers was highly detected and maintained within the core population with small size and low cytoplasmic complexity. The core population expressed cytokeratin 7 and cytokeratin 14, known as duct markers indicating that Epi-SGs might be originated from the duct. When Epi-SGs were transplanted in vivo with Matrigel, acini-like structures were readily formed at 4 days after transplantation and they were maintained at 7 days after transplantation. Taken together, our data suggested that Epi-SGs might contain stem cells which were positive for EpiSC and ESC markers, and Epi-SGs might contribute to the regeneration of acini-like structures in vivo. We expect that Epi-SGs will be useful source for the functional restoration of damaged salivary gland.

Preparation of Active Human HtrA3 in Eschrichia coli and Comparison of Proteolytic Activity between HtrA1, 2, and 3 (Escherichia coli에서 효소활성을 지닌 Human HtrA3 단백질 제조와 HtrA Serine Protease 1, 2와의 효소활성 비교)

  • Kim, Ji-Hwan;Kim, Goo-Young;Nam, Min-Kyung;Kim, Sang-Soo;Rhim, Hyang-Shuk
    • Korean Journal of Microbiology
    • /
    • v.45 no.4
    • /
    • pp.291-299
    • /
    • 2009
  • To elucidate HtrA3's functional roles in the HtrA3 mediated cellular processes, it is necessary to investigate its biochemical characteristics. In the present study, we constructed the plasmids encoding putative mature HtrA3 proteins (M1-HtrA3 and M2-HtrA3) based on the putative maturation sites of highly homologous HtrA1 and mouse HtrA3. We used the pGEX bacterial expression system to develop a simple and rapid purification for the recombinant HtrA3 protein. Although yields of the mature HtrA3 proteins were slightly low as 10~50 ${\mu}g$/L, the amounts and purity of M1- and M2-HtrA3 were enough to investigate their proteolytic activities. The putative mature HtrA3 proteins have proteolytic activity which could cleave $\beta$-casein as an exogenous substrate. We compared the proteolytic activity between the HtrA family, HtrA1, HtrA2, and HtrA3. The cleavage activity of HtrA3 and HtrA2 were 2 folds higher than that of HtrA1, respectively. Our study provides a method for generating useful reagents to identify natural substrates of HtrA3 in the further studies.