• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.93-96
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• 2011

It has been shown that a nucleotide substitution at position 770 in Escherichia coli 16S rRNA, which is implicated in forming the evolutionary conserved B2c intersubunit bridge, has a detrimental effect on ribosome function. In order to isolate second-site revertants that complement ribosomes containing C770G, we performed a random mutagenesis of the 16S rRNA gene and selected clones that could produce more CAT protein translated by specialized ribosome. One of the clones contained two nucleotide substitutions at positions 569 and 904 (C569G and U904C) and these mutations partially complemented the loss of protein-synthesis ability caused by C770G. Further studies using the isolated revertant will provide information about which part of 16S rRNA is interacting with C770 and the consequence of the structure formed by these interactions in the process of protein synthesis.

• The Journal of Korean Academic Society of Nursing Education
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• v.9 no.1
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• pp.144-154
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• 2003

Recently, in the academic disciplines critical thinking has been emphasized as the new evolution in education. Problem-based learning is suggested as the evolutionary learning method in developing critical thinking. In the nursing education, PBL is offered as an appropriate teaching method to prepare nurse student for professional. PBL is a student-centered learning strategy aimed at developing critical thinking, motivating self-directed study and attaining autonomy. The purpose of this study was to develop the communication curriculum of nursing education based on PBL and implementing of it. PBL module was developed focused on five communication situations. And learning strategies to facilitate the learning process and the guided questions to stimulate student inquiry were also developed. This PBL education was conducted for six students in the master's course during the 14 weeks from March, 2000 to June, 2000. The outcomes of the PBL education were examined based on the content analysis of the students reflective journal. As a result, it was found that students experienced the effect of it focused on self-oriented, group-oriented, and practice-oriented domain. In the self-oriented domain, the findings indicate that there were 5 kinds of concepts including 'motivation for self-directed study', 'transition toward certainty in knowledge acquisition', 'attempt to apply their prior knowledge into new situation', 'enhancement of self-image in real situation', 'self-growth with self reflection'. In the group-oriented domain, there were 3 kinds of concepts including 'cognitive work in group', 'perception of co-responsibility in attaining learning objectives', 'socialization with group members'. In the practice-oriented domain, there were 3 kinds of concepts including 'linkage theoretical knowledge with real situation', 'attempt to apply in real situation', 'development problem solving skill in real situation'. In conclusion, PBL had a significant effect on self, group, and clinical domain. And assessing PBL outcomes is challenging because standardized instrument do not develop yet. So the findings of this study can suggest the basic data for examining the PBL outcome.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.97-103
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• 2011

Two Gram positive bacterial strains, designated strain GC-1 and GC-4, were isolated from coastal seawater near Jeju Island in the Republic of Korea. The two strains were identified as members of the genus Bacillus, based on 16S rRNA gene sequencing and data for physiological characteristics analyses. A subtle difference in physiological and genotypical characteristics has led us to designate the strains GC-1 and GC-4. The strain GC-1 showed a 99.91% similarity in 16S rRNA gene sequencing with B. tequiliensis and B. subtilis subsp. inaquosorum and the strain GC-4 showed a 100% similarity in 16S rRNA gene sequencing with those of B. altitudinis, B. stratosphericus, and B. aerophilus. However, both strains exhibited different physiological and genotypical characteristics in many aspects from those of their phylogenetically closest neighbors listed above, which implies that genus Bacillus has diversified into various species during its evolutionary process.

• Journal of Labour Economics
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• v.27 no.1
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• pp.27-53
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• 2004

This paper analyzes the two cases of labor disputes (Hyundai Motor in 1998 and Power Generation Companies in 2002) in the period of restructuring, by applying the behavioral theory of labor negotiations as a comparative framework. The paper compares th backgrounds of the labor disputes, core issues, bargaining processes, and evolutionary patterns and consequences of the labor disputes at the two cases. The common features, found in the two dispute cases, are strong mistrust and exclusive bargaining attitude between labor unions and management, little feasibility of contract zone in bargaining proposals by the two parties, heteronomous dispute resolution by the intervention of the government, and the lack of learning effect gained from the experience of labor disputes. This comparative case study identifies that the confrontational labor-management relations at the firm level is re-produced by a regressive process of the following circulation: labor-management distrust $\rightarrow$ interest conflict in bargaining demand $\rightarrow$ exclusive bargaining attitude $\rightarrow$ the experience of antagonistic dispute $\rightarrow$ deepened distrust. In conclusion, four parties-labor unions, management, the government, and public press - are required to make much effort to replace the vicious circle of labor-management confrontation by a virtueous cycle of labor-management cooperation.

• Journal of Aquaculture
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• v.20 no.2
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• pp.140-143
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• 2007

Cytogenetic analyses of an endemic species, Coreoleuciscus splendidus (Cyprinidae) was performed including erythrocyte measurement, chromosome count and karyotyping, nucleolar organizing region (NOR) banding and flow cytometric analysis of genome size. C. splendidus had the same modal chromosome number of 2n = 48 between sexes, however, displayed a sex-related dimorphism in their chromosome karyotypes. Males represented a pair of heteromorphic chromosomes which couldn‘t be seen in any female individuals, indicating that the sex determination mechanism of this species should be a typical XX-XY based male heterogamety (female=10M+6SM+8A+XX vs male=10M+6SM+8A+XY). Other cytogenetic features such as Ag-NORs located in a pair of acrocentric chromosomes, estimated nuclear volume ($28{\mu}m^3$) and cellular DNA content (2.4 pg/cell) suggest that genetic recombination might be the main driving force responsible for the evolution of this species rather than the polyploidy-based evolutionary process as in many other Cyprinidae species.

• Spatial Information Research
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• v.20 no.1
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• pp.9-18
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• 2012

South Korea has many landslides caused by heavy rains during summer time recently and the landslides continue to cause damages in many places. These landslides occur repeatedly each year, and the frequency of landslides is expected to increase in the coming future due to dramatic global climate change. In Korea, 81.5% of the population is living in urban areas and about 1,055 million people are living in Seoul. In 2011, the landslide that occurred in Seocho-dong killed 18 people and about 9% of Seoul's area is under the same land conditions as Seocho-dong. Even the size of landslide occurred in a city is small, but it is more likely to cause a big disaster because of a greater population density in the city. So far, the effort has been made to identify landslide vulnerability and causes, but now, the new dem and arises for the prediction study about the areal extent of disaster area in case of landslides. In this study, the diffusion model of the landslide disaster area was established based on Cellular Automata(CA) to analyze the physical diffusion forms of landslide. This study compared the accuracy with the Seocho-dong landslide case, which occurred in July 2011, applying the SCIDDICA model and the CAESAR model. The SCIDDICA model involves the following variables, such as the movement rules and the topographical obstacles, and the CAESAR model is also applied to this process to simulate the changes of deposition and erosion.

• Molecules and Cells
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• v.28 no.6
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• pp.529-536
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• 2009

Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.

• Journal of Haehwa Medicine
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• v.22 no.2
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• pp.1-12
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• 2014

The Sasang constitutional Medicine is the original medicine that is created from the deep studies on former Eastern medical theories by Lee Jae-Ma in the late 19th century. This medicine deals with the interaction between mind and body in great depth. The temperament (the distinct nature and character of an individual, 性情) concept is the theoretical basis which divides man's constitution into four(Taeyangin, Taeumin, Soyangin and Soumin). This concept is derived from The old oriental Sung-Myung concept which has philosophical meaning. These terms of Sung and Jung also have metaphysical meaning and can not be explained easily. but roughly, The temperament is divided two concept. the distinct nature(性) means human's nature and the distinct nature(情) means human's desire. Besides, In Sasang constitution medicine, terms that traditionally represent emotions in asia are used as terminology of temperament. Altough too many aspects about Human's mind remains unknown yet, According to Neurological evidences, the brain is regarded as the main organ that produces rationality and emotion. Especially the way that brain produces an emotion provides some clues that can tell us how can mind affect body. Emotion is considered as evolutionary adaptation to response correctly against unexpected chaotic external changes. It is something that humans are born with, and causes physical responses simultaneously. Moreover, It can be come out with or without consciousness. The temperament(性情) concept and Emotion have similarity that both play an important role in mind-body correlation. Therefore Neurological researches on emotion were able to help reinterpret temperament(性情) concept. the distinct nature(性) seems to be the emotion that is come out directly from the brain stem, and the distinct nature(情) seems to be the emotion that is produced after neocortex involved thinking process. And the reason why Sung and Jung affect organs differently is explained from the manner that brain expresses emotion.

• Journal of the Korean association of regional geographers
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• v.23 no.2
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• pp.239-256
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• 2017

This research is a theoretically grounded empirical study aimed at shedding light on the policy evolution of Technology Park(TP) in term of historical institutionalism in South Korea for the 16 years. The study analysed the evolution of TP, and the empirical fieldwork was completed in two regions, Gyeongsangbuk-do ana Daegu City. Historically the policy can be divided into three periods being affected by endogenous and exogenous impact. This research found that the functions of local TPs changed as following: six functions decided by central governments ${\rightarrow}$ added the role of supporting regional innovation ${\rightarrow}$ comprehensive support for small & medium-sized enterprise. Analysis of the empirical study reveals significant findings: first, the relationships between central government and local actors which was built in the quickening period have been path dependency. Second, the functions of local TPs were evolved with the characteristics of layering and reorientation of the institutions. Thirdly, there were some differentiation between the two regions because of the milieu, though the central government had the power deciding the functions.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.13-22
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• 2003

Growth Hormone (GH) gene is a member of gene family through the evolutionary process from a small common ancestral gene by a series of gene duplications. The role of the GH in growth and performance controls has been extensively studied in human, mice and livestock. Many researchers have considered GH as a strong candidate gene for evaluation of genetic polymorphisms that could be associated with economic traits in cattle. We report here a novel missense mutation within the exon 5 of the bovine Growth Hormone (bGH) gene. We could amplified 522 bp fragments from eight unrelated Hanwoo cattle by PCR, then, subsequently cloned and sequenced. An Msp I RFLP corresponding to a C to T transition was observed at position 2258 nt. From this result, we could predict a missense mutation (Arg to Trp) at codon 166 in a highly conserved region among many mammals. Codominant Mendelian segregation of the two alleles, Msp I (+) and Msp I (－), was observed in two full-sib F2 families (n = 32, African taurine Bos taurus ${\times}$ African zebu Bos indicus) and eight half-sib Hanwoo families. For the availability of genetic marker, we have performed PCR-RFLP with a large number of individual animals from 15 different cattle breeds (European and Asian taurines, and African indicines). Consideration of breed frequencies of Msp I (－) allele in relation to breed type and their geographic origins, shows higher frequencies in humped breeds or Asian cattle breeds than in humpless or European breeds. This result indicates that the missense mutation can be contributed the functional significance such as the signal transduction through the receptor binding, also may be used as a marker for selection of the economic traits in Hanwoo.