• Bulletin of the Korean Chemical Society
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• 제35권3호
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• pp.933-937
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• 2014

A derivative of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) has been used for the preparation of tuberculosis vaccines. To establish a Korean tuberculosis vaccine derived from BCG-Pasteur $1173P_2$, genome sequencing of a BCG-Korea strain was completed by Joung and coworkers. A comparison analysis of the genome sequences of the BCG-Pasteur $1173P_2$ and BCG-Korea strains showed marginal increases in the total genome length (~0.05%) and the number of genes (~4%) in the BCG-Korea genome. However, how the genomic changes affect the BCG-Korea protein expression levels remains unknown. Here, we provide evidence of the proteomic alterations in the BCG-Korea strain by using a SWATH-based mass spectrometric approach (Sequential Window Acquisition of all THeoretical mass spectra). Twenty BCG proteins were selected by top-rank identification in the BCG proteome analysis and the proteins were quantified by the SWATH method. Thirteen of 20 proteins showing significant changes were enough to discriminate between the two BCG proteomes. The SWATH method is very straightforward and provides a promising approach owing to its strong reliability and reproducibility during the proteomic analysis.

• Molecules and Cells
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• 제38권3호
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• pp.202-209
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• 2015

Hepatocellular carcinoma (HCC), a highly malignant disease and the third leading cause of all cancer mortalities worldwide, often responses poorly to current treatments and results in dismal outcomes due to frequent chemoresistance and tumor relapse. The heterogeneity of HCC is an important attribute of the disease. It is the outcome of many factors, including the cross-talk between tumor cells within the tumor microenvironment and the acquisition and accumulation of genetic and epigenetic alterations in tumor cells. In addition, there is accumulating evidence in recent years to show that the malignancy of HCC can be attributed partly to the presence of cancer stem cell (CSC). CSCs are capable to self-renew, differentiate and initiate tumor formation. The regulation of the stem cell-like properties by several important signaling pathways have been found to endow the tumor cells with an increased level of tumorigenicity, chemoresistance, and metastatic ability. In this review, we will discuss the recent findings on hepatic CSCs, with special emphasis on their putative origins, relationship with hepatitis viruses, regulatory signaling networks, tumor microenvironment, and how these factors control the stemness of hepatic CSCs. We will also discuss some novel therapeutic strategies targeted at hepatic CSCs for combating HCC and perspectives of future investigation.

• Archives of Plastic Surgery
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• 제40권4호
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• pp.302-303
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• 2013

Current educational interventions and training courses in microsurgery are often predicated on theories of skill acquisition and development that follow a 'practice makes perfect' model. Given the changing landscape of surgical training and advances in educational theories related to skill development, research is needed to assess current training tools in microsurgery education and devise alternative methods that would enhance training. Simulation is an increasingly important tool for educators because, whilst facilitating improved technical proficiency, it provides a way to reduce risks to both trainees and patients. The International Microsurgery Simulation Society has been founded in 2012 in order to consolidate the global effort in promoting excellence in microsurgical training. The society's aim to achieve standarisation of microsurgical training worldwide could be realised through the development of evidence based educational interventions and sharing best practices.

• 약학회지
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• 제51권5호
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• pp.301-306
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• 2007

Rosiglitazone ($Avandia^{(R)}$) represents a new class of antidiabetic drugs which are $PPAR{\gamma}$ agonists. The present study was undertaken to determine whether the new antidiabetic rosiglitazone influences on the agonist-induced regulation of vascular smooth muscle contraction as an antihypertensive and, if so, to investigate the related mechanism. Endothelium-denuded arterial rings from male Sprague-Dawley rats were used and isometric contractions were recorded using a computerized data acquisition system. Rosiglitazone decreased Rho-kinase activating agonist (NaF or thromboxane $A_2$ mimetic)-induced contraction but not depolarization- or phorbol ester-induced contraction. Surprisingly, it slightly potentiated the latter contraction possibly opening a voltage-dependent calcium channel by its chemical structure on 50 mM KCI- or $1{\mu}M$ phorbol 12,13-dibutyrate-induced vasoconstriction. In conclusion, this study provides the evidence and possible related mechanism concerning the biphasic effect of an antidiabetic rosiglitazone as a possible antihypertensive on the agonistinduced contraction in rat aortic rings regardless of endothelial function.

• 환경영향평가
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• 제14권5호
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• pp.317-335
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• 2005

This paper is primarily intended to propose a new concept of "aggregate control of road density" which is defined as the area-wide road surface per watershed area. An empirical study for experimental sites was conducted to confirm how a standard GIS technology can be used to assist in estimating the road density in terms of total volume threshold control. Guidelines for a replicable methodology are presented to provide a strong theoretical basis for the standardization of factors involved in the estimation of the road space threshold; the meaningful classification of road types, delination of watershed boundary, interpretation for distribution trends of road density etc. A variety of visual maps using overlay analysis can be generated over large areas quickly and easily to show the fact that some degree of road space already exists in the experimental sites. They could be used as an evidence to limit further construction of road network in comparison with other tributary watershed. It is anticipated that this research output could be used as a valuable reference to confirm the potential of introducing the new concept of "site specific road density" to support more scientific and objective decision-making in the process of road construction project.

• 한국언어정보학회지:언어와정보
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• 제5권2호
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• pp.39-55
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• 2001

This paper investigates the idiosyncratic understanding of universal quantifiers such as every, each or all by young children at the ages of 4 to 7, and argues that the phenomenon is explicable in terms of the maturation of both the cognitive system and the linguistic system. Evidence for this dual explanation comes from the fact that the visual input, a picture, plays a key role in determining the children's conceptual representation, suggesting the need for the central integration of visual and linguistic elements; and from the fact that a quantifier in the linguistic input has an intrinsic property, i.e. a <+focus> feature. I have tried to explain the nature of the cognitive factors in terms of the function of the central system, suggesting a modified form of Smith & Tsimpli's (1995) yersion of Fodor's (1983) modularity hypothesis. The categorial status of the quantifier in the children's interpretation is considered, focusing on the movement of that quantifier out of its own extended projection to FP. It is claimed that children initially treat quantifiers as modifiers, rather than functional heads, and that the phenomenon of quantifier spreading by children can be attributed to delay in the development of the relevant functional category, i.e., DP (or QP), in language acquisition.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.352-356
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• 2012

The present study was undertaken to determine whether ethanol influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Ethanol significantly inhibited thromboxane $A_2$ mimetic-induced contraction with intact endothelial function, but there was no relaxation on thromboxane $A_2$ mimetic U-46619-induced contraction irrespective of endothelium suggesting that the pathway such as Rho-kinase activation, $Ca^{2+}$ entry or thin filament regulation was not affected. In addition, ethanol didn't decrease thromboxane $A_2$ mimetic-induced increase of phospho-myosin phosphatase targeting subunit protein 1 (pMYPT1) or pERK1/2. Interestingly, ethanol didn't inhibit significantly phorbol ester-induced contraction in denuded muscles suggesting that thin filament regulation is less important on the ethanol-induced regulation in the muscle than endothelial NO synthesis. In conclusion, this study provides the evidence and possible related mechanism concerning the effect of ethanol on the agonist-dependent contraction in rat aortic rings with regard to endothelial function.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2014년도 춘계학술발표대회
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• pp.457-460
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• 2014

클라우드 컴퓨팅은 IT 자원의 효율적인 관리와 비용 대비 양질의 서비스 제공을 위한 새로운 패러다임으로써, 국내외의 기업뿐만 아니라 많은 사용자들에게 주목 받고 있다. 하지만 관련 시장의 빠른 성장과 함께 다양한 사이버 범죄에 노출될 수 있는 위험이 높아졌음에도 불구하고, 클라우드 컴퓨팅에 대한 디지털 포렌식은 실질적인 역할을 수행하기에 아직 미비한 실정이다. 클라우드 컴퓨팅은 증거 데이터가 물리적으로 분산되어 있고, 자원이 가상공간에 존재할 수 있기 때문에 기존의 디지털 포렌식 수사와는 다르게 접근해야 한다. 이에, 본 논문에서는 추상화된 클라우드 계층에 따른 기존 포렌식 절차 상의 데이터 수집 방법에 관한 한계를 분석하고, 확보한 증거 데이터의 신뢰성 보장 및 다양한 클라우드 환경에 보다 유연하게 적용할 수 있는 디지털 증거 수집 절차를 제안한다. 해당 절차는 클라우드 구성 요소들 중 물리적인 자원들을 가상화하여 논리적으로 구성할 수 있도록 하며, 가상화된 자원들을 서비스 목적에 따라 폭넓게 활용할 수 있도록 관리 체계를 제공해주는 클라우드 플랫폼을 기반으로 한다.

• The Journal of Asian Finance, Economics and Business
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• 제6권2호
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• pp.173-181
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• 2019

The paper aims to evaluate the combination of strategies that influence the success of New Technology Based Firms (NTBFs) located in isolated areas with resource constraints. We utilize the Kauffman Firm Survey to construct a subset of 224 firms operating in primary metal manufacturing industries and who are located in non-thriving areas. We focus on the impact of technological strategy, in the form of Inward Technology Licensing (ITL), combined with financial strategy, in the form of increased financial slack. Using a negative binomial-specification technique to model these relations, we find that ITL positively impacts internal innovation in the firm and this relation is strengthened by the presence of greater financial slack. This positive impact of financial slack supports the behavioral theory of the firm rather than agency theory in that financial slack enables further innovation rather than stifling it. This research confirms the importance of resource acquisition, suggesting that entrepreneurs may utilize external sources of knowledge in an effort to build a favorable situation when facing the challenges of location. Finally, by presenting evidence showing the compatibility of a financial strategy with a knowledge strategy (ITL), this study emphasizes the importance to entrepreneurs of choosing the proper combination of varying strategies.

• 말소리와 음성과학
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• 제12권4호
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• pp.9-17
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• 2020

Changes in the first language (L1) sound system as a result of acquiring a second language (L2) (i.e., phonetic drift) have received considerable attention from a variety of speakers, settings, and environments. Less attention has been given to phonetic drift in adult speakers' L2 learning as their length of residence in America (LOR) increases. This study examines the effects of LOR on voice onset time (VOT) in L1 Korean stops. Three different groups of Korean adult learners of L2 English were compared to assess how malleable their L1 representations are in terms of LOR and whether there is any relationship between L1 change and L2 acquisition. The results showed that the effect of LOR was linguistically unimportant in the production of Korean stops. However, VOT merger as evidence of sound change in Korean stops were robust in the speech production of most of the female speakers across the groups. The results suggest that L2 English may not be the primary cause of L1 sound change. For generalizability, further study is necessary to see whether other acoustic cues show a similar pattern.