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http://dx.doi.org/10.4062/biomolther.2012.20.3.352

Controversial Effect of Ethanol Irrespective of Kinases Inhibition on the Agonist-Dependant Vasoconstriction  

Je, Hyun-Dong (Department of Pharmacology, College of Pharmacy, Catholic University of Daegu)
Kim, Hyeong-Dong (Department of Physical therapy, College of Health Science, Korea University)
Park, June-Hong (Department of Food and nutrition, College of Human Ecology, Yeungnam University)
Publication Information
Biomolecules & Therapeutics / v.20, no.3, 2012 , pp. 352-356 More about this Journal
Abstract
The present study was undertaken to determine whether ethanol influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Ethanol significantly inhibited thromboxane $A_2$ mimetic-induced contraction with intact endothelial function, but there was no relaxation on thromboxane $A_2$ mimetic U-46619-induced contraction irrespective of endothelium suggesting that the pathway such as Rho-kinase activation, $Ca^{2+}$ entry or thin filament regulation was not affected. In addition, ethanol didn't decrease thromboxane $A_2$ mimetic-induced increase of phospho-myosin phosphatase targeting subunit protein 1 (pMYPT1) or pERK1/2. Interestingly, ethanol didn't inhibit significantly phorbol ester-induced contraction in denuded muscles suggesting that thin filament regulation is less important on the ethanol-induced regulation in the muscle than endothelial NO synthesis. In conclusion, this study provides the evidence and possible related mechanism concerning the effect of ethanol on the agonist-dependent contraction in rat aortic rings with regard to endothelial function.
Keywords
Ethanol; Phorbol ester; Rho-kinase; Thromboxane $A_2$ mimetic; Vasodilation;
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