• 사상체질의학회지
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• 제12권1호
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• pp.186-200
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• 2000

소양인(少陽人) 인동등지골피탕(忍冬藤地骨皮湯)이 중소(中消) 합병증(合倂症)에 미치는 영향(影響)을 살펴보고자 하였고 결과(結果)는 다음과 같다. 1. 장관운동(腸管運動)을 억제(抑制)하였다. 2. Acetic acid에 의한 writhing syndrome을 억제(抑制)하였다. 3. Evans blue에 의한 모세혈관투과성(毛細血管透過性)을 억제(抑制)하였다. 4. Histamine에 의한 급성족부종(急性足浮腫)을 억제(抑制)하였다. 5. Cotton pellet에 의한 육아종(肉芽腫) 형성(形成)을 억제(抑制)하였다. 6. 흉선세포(胸線細胞) 및 비장세포(脾臟細胞)의 세포생존율(細胞生存率)을 증가(增加)시켰다. 7. 흉선세포(胸線細胞)로부터 ${\gamma}-IFN$ 및 IL-2의 분비(分泌)를 억제(抑制)하였다. 8. 비장세포(脾臟細胞)로부터 IL-4의 분비(分泌)는 증가(增加)시켰으나, $TNF-{\alpha}$ 및 $IL-1{\beta}$의 분비(分泌)는 억제(抑制)하였다. 9. 복강(腹腔) 대식세포(大食細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 분비(分泌)를 억제(抑制)하였다. 10. 복강(腹腔) 대식세포(大食細胞)의 lucigenin chemiluminescence 및 FITC-conjugated E. cole engulfment를 촉진(促進)하였다. 이상의 실험(實驗) 결과(結果) IJTE는 장관운동(腸管運動)을 억제(抑制)하였으며, 진통(鎭痛) 및 항염작용(抗炎作用)을 나타냈고, 이들 작용은 면역세포(免疫細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 생성(生成)을 억제(抑制)하기 때문인 것으로 추정된다. 따라서 이러한 기전(機轉)에 의하여 인동등지골피탕(忍冬藤地骨皮湯)은 중소(中消) 합병증(合倂症)의 하나인 염증(炎症)을 억제(抑制)하는데 유효(有效)한 것으로 사료(思料)된다.

• 동의생리병리학회지
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• 제18권3호
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• pp.857-862
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• 2004

The purpose of this research was to investigate the effects of supercritical fluid extract of Kamichungbieum (SFE) on allergic reaction. SFE (500 mg/kg) inhibited the systemic anaphylaxis induced by compound 48/80 or platelet activating factor and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-lgE and DNP-human serum albumin (HSA) in vivo. Also, SFE inhibited the SRSC-induced delayed type hypersensitivity and inhibited the hind paw edema induced by histamine. In addition, SFE inhibited the permeability of evans blue induced by acetic acid and inhibited the writhing syndrome induced by acetic acid. These results indicate that SFE may be useful for the prevention and treatment of allergy related disease.

• 동의생리병리학회지
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• 제17권5호
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• pp.1188-1193
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• 2003

Sogunjung-tang(SGJT) has been used for the treatment of general weakness, digestive organ disease and so on. This study was carried out for the purpose of knowing the inhibitory effect on the Type I hypersensitivity and Inflammatory reactions of SGJT. The reasults were obtained as follows: SGJT(0.1, 0.5, 1, 2mg/g) concentration of dependently inhibited compound48/80 induced anaphylaxis reaction in mice. SGJT(2mg/g) also inhibited permeability of evans blue into peritoneal cavity in mice. SGJT reduced IgE, CRP, WBC and Platelets on egg albumin induced hypersensitivity. but serum NO was grown. According to above results, SGJT may be beneficial in the type I hypersensitivity and Inflammatory reactions by inhibition of histamine release from mast cells.

• 동의생리병리학회지
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• 제17권5호
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• pp.1293-1298
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• 2003

HGJT has been used for the treatment of general weakness, digestive organ disease and so on. This study was carried out for the purpose of knowing the inhibitory effect on the Type I hypersensitivity and Inflammatory reactions of Hwanggigunjungtang(HGJT). The reasults were obtained as follows: HGJT(0.1. 0.5. 1, 2mg/g) concentration of dependently inhibited compound48/80 induced anaphylaxis reaction in mice. HGJT(2mg/g) also inhibited permeability of evans blue into peritoneal cavity in mice. HGJT reduced IgE, CRP. WBC and Platelets on egg albumin induced hypersensitivity. But serum NO was grown. According to above results, HGJT may be beneficial in the type I hypersensitivity and Inflammatory reactions by inhibition of histamine release from mast cells.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.83-88
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• 2001

A cell culture system of poplar (Populus alba x P.glandulosa) was established to test four different methods for evaluation of cellular stresses. Two different kinds of stresses were given to the cultures by adding either Pb(NO$_3$)$_2$ or paraquat and the cellular responses were monitored during a week period. While fresh weight reduction was observable in two days after the treatment of Pb(NO$_3$)$_2$, such changes were apparent only in later stage in paraquat treated cultures. Cells in paraquat treated cultures in the first 3 days showed no alteration in fresh weight as compared to untreated cultures, but had their MTT reducing activities completely inhibited. Neither Evans blue staining nor ion conductivity of the medium was consistent with fresh weight changes of the cultures. Overall, cell clumps formed during suspension culture appeared to interfere with staining and washing reactions and thus cause the assays unreliable. Among the four methods examined, fresh weight changes and MTT reducing activity appeared to be the most reliable and consistent.

• Toxicological Research
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• 제23권3호
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• pp.239-244
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• 2007

Respirable particles cause many occupational and environmental diseases of the lung. To study these diseases, laboratory animals are often exposed to these particles. Inhalation and instillation are the well-known techniques for experimental exposures of the lung to respirable particles. Recently, another technique called oropharyngeal aspiration (OPA) has been introduced for exposing the lung to pathogens and/or particles. The conventional OPA technique for the mouse is generally carried out using a micropipette with a fixed slant board. In order to modify the conventional OPA in this study, anesthetized mice were placed on an adjustable slant board, a syringe pump was used to deliver the solution to the oropharynx, and the mice were allowed to recover in vertically positioned tubes for 6 minutes until fully awaked. Most importantly, the whole process of OPA could be carried out simply by an examiner. This modified OPA technique was validated by exposing the mouse lung to Evans Blue dye with a success rate of 95%.

• The Plant Pathology Journal
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• 제32권4호
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• pp.340-346
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• 2016

To understand how Bursaphelenchus xylophilus kills pine trees, the differences between the effects of B. xylophilus and B. mucronatus on pine trees are usually compared. In this study, the migration and attacking ability of a non-pathogenic B. mucronatus in Pinus thunbergii were investigated. The distribution of B. mucronatus and the number of dead epithelial cells resulting from inoculation were compared with those of the pathogenic B. xylophilus. Although B. mucronatus is non-pathogenic in pines, its distribution pattern in P. thunbergii was the same as that of B. xylophilus. We therefore concluded that the non-pathogenicity of B. mucronatus could not be attributed to its migration ability. The sparse and sporadic attacking pattern of B. mucronatus was also the same as that of B. xylophilus. However, the number and area of the dead epithelial cells in pine cuttings inoculated with B. mucronatus were smaller than in those cuttings inoculated with B. xylophilus, meaning that the attacking ability of B. mucronatus is weaker than that of B. xylophilus. Therefore, we concluded that the weaker attacking ability of B. mucronatus might be the factor responsible for the non-pathogenicity.

• 동의생리병리학회지
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• 제19권6호
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• pp.1604-1609
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• 2005

The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.

• 대한수의학회지
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• 제16권2호
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• pp.187-190
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• 1976

In order to approximate plasma volume, extracellular fluid volume and total body water volume of Korean native goats, measurements were made of the volumes of distribution of Evans blue, potasium thiocyanate and antipyrine. The results obtained in this work were summarized as follows: 1. Plasma volume showed a range of 50 to 72ml/kg with a mean of $62{\pm}6.2ml/kg$ (SD). 2. Blood volume showed a range of 69 to 98ml/kg with a mean of $85{\pm}7.9ml/kg$. 3. Extracellular fluid volume showed a range of 265 to 310ml/kg with a mean of $297{\pm}18.3ml/kg$. 4. Interstitial fluid volume showed a range of 204 to 261ml/kg with a mean of $236{\pm}16.8ml/kg$. 5. Intracellular fluid volume showed a range of 380 to 436ml/kg with a mean of $420{\pm}12.6ml/kg$. 6. The volume of total body water showed a range of 680 to 735ml/kg with a mean of $714{\pm}17.7ml/kg$.

• 한방안이비인후피부과학회지
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• 제6권1호
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• pp.53-70
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• 1993

These experiments were conducted to investigate the effect of Taklee Hwangki Tang(THT) on inflammation. THT extract did not affected on the leakage of evans blue into peritoneal cavity and mouse paw edema induced by histamine, but decreased the cottom pellet granuloma formation. Using proliferation of Balb/c 3T3 fibroblast cell line as an in vitro model of granulation tissue formation, the ability of THT to stumulate cellular proliferation of fibroblast cells was investigated. When the cells were seeded at $1{\times}10^4$ cells/well, balb/c 3T3 cells are reached to the late expponential phase at 3rd day. Under the conditions established above, THT increased the proliferation of Balb/c 3T3 cells at concentration of $10^-,\;10^{-6}\;and\;10^{-5}g/ml$. The treatment of $10^{-6}g/ml$ of THT did not influence onthe NDA syntesis and proteinsynthesis of the cells. The $10\%$ serum from THT treated mice(500mg/kg/day for 4 days) increased the proliferation of Balb/c 3T3 fibroblast markedly, but decreased the DNA synthesis and protein sythesis of the cells. The results suggest that THT may be of practical therapeutic use at the period of the last in. flammation.