Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
권호 표지

Effecs of Soyangin Indongdeungjikolpitang on the Complication of Diabetes

소양인(小陽人) 인동등지골피탕(忍冬藤地骨皮湯)이 중소합병증(中消合倂症)에 미치는 영향(影響)

  • Seo, Yong-bae (Dept. of Oriental medicine, Graduate School of Woo Suk University) ;
  • Song, Jeong-mo (Dept. of Oriental medicine, Graduate School of Woo Suk University)
  • 서용배 (우석대학교 한의과대학 사상체질의학교실) ;
  • 송정모 (우석대학교 한의과대학 사상체질의학교실)
  • Published : 2000.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this research was to investigate the effects of Indongdeungjikolpitang water extract(IJTE) on the complication of diabetes. IJTE did not affect the level of blood glucose in alloxan- or streptozotocin-induced hyperglycemic mice, but inhibited the motility of gastrointestine. IJTE inhibited the writhing syndrome induced by acetic acid, the permeability of evans blue into peritoneal cavity induced by acetic acid, the paw edema induced by histamine, and the formation of cotton pellet granuloma. IJTE increased the cell viability of thymocytes and splenocytes. IJTE decreased the release of ${\gamma}-interferone$(${\gamma}-IFN$) and interleukin-2(IL-2), but did not affect the release of interleukin-4(IL-4) from murine thymocytes. IJTE increased the release of IL-4 and decreased the release of tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) and $interleukin-1{\beta}$($IL-1{\beta}$), but did not affect of ${\gamma}-IFN$ and IL-2 from murine splenocytes. IJTE decreased the release of $TNF-{\alpha}$ and $IL-1{\beta}$ from murine peritoneal macrophages. IJTE decreased the production of niric oxide(NO) from murine peritioneal macrophages and increased the phagocytic activity of murine peritoneal macrophages. These results suggest that IJTE has an anti-inflammatory action via the inhibition of $TNF-{\alpha}$, $IL-1{\beta}$ and NO production from immune cells.

소양인(少陽人) 인동등지골피탕(忍冬藤地骨皮湯)이 중소(中消) 합병증(合倂症)에 미치는 영향(影響)을 살펴보고자 하였고 결과(結果)는 다음과 같다. 1. 장관운동(腸管運動)을 억제(抑制)하였다. 2. Acetic acid에 의한 writhing syndrome을 억제(抑制)하였다. 3. Evans blue에 의한 모세혈관투과성(毛細血管透過性)을 억제(抑制)하였다. 4. Histamine에 의한 급성족부종(急性足浮腫)을 억제(抑制)하였다. 5. Cotton pellet에 의한 육아종(肉芽腫) 형성(形成)을 억제(抑制)하였다. 6. 흉선세포(胸線細胞) 및 비장세포(脾臟細胞)의 세포생존율(細胞生存率)을 증가(增加)시켰다. 7. 흉선세포(胸線細胞)로부터 ${\gamma}-IFN$ 및 IL-2의 분비(分泌)를 억제(抑制)하였다. 8. 비장세포(脾臟細胞)로부터 IL-4의 분비(分泌)는 증가(增加)시켰으나, $TNF-{\alpha}$ 및 $IL-1{\beta}$의 분비(分泌)는 억제(抑制)하였다. 9. 복강(腹腔) 대식세포(大食細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 분비(分泌)를 억제(抑制)하였다. 10. 복강(腹腔) 대식세포(大食細胞)의 lucigenin chemiluminescence 및 FITC-conjugated E. cole engulfment를 촉진(促進)하였다. 이상의 실험(實驗) 결과(結果) IJTE는 장관운동(腸管運動)을 억제(抑制)하였으며, 진통(鎭痛) 및 항염작용(抗炎作用)을 나타냈고, 이들 작용은 면역세포(免疫細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 생성(生成)을 억제(抑制)하기 때문인 것으로 추정된다. 따라서 이러한 기전(機轉)에 의하여 인동등지골피탕(忍冬藤地骨皮湯)은 중소(中消) 합병증(合倂症)의 하나인 염증(炎症)을 억제(抑制)하는데 유효(有效)한 것으로 사료(思料)된다.

Keywords

References

  1. 東醫壽世保元 李濟馬
  2. 糖尿病과 消渴 申載鏞
  3. 韓方臨床四十年 朴炳昆
  4. 東洋醫學 v.4 糖尿病의 韓方療法 金永萬
  5. 四象醫學會誌 v.1 no.1 糖尿病의 心身醫學的 側面에 對한 四象醫學的 考察 金龍國
  6. 大韓韓醫學會誌 v.2 no.1 韓醫學的으로 본 糖尿病의 槪說 金應模
  7. 東醫內科學 朴稿湜(등)
  8. 東醫腎系內科學 杜鎬京
  9. 東醫寶鑑 許浚
  10. 丹溪心法 朱震亨
  11. 中國醫學大系 v.14 世醫得效方 危亦林
  12. 四象醫學會誌 v.10 no.2 少陽人 凉膈散火湯과 忍冬藤地骨皮湯 및 熟地黃苦蔘湯이 Alloxan投與 高血糖 白鼠에 미치는 影響 崔炳一
  13. 四象醫學會誌 v.2 no.1 少陽人에 應用되는 忍冬藤地骨皮湯이 streptozotocin投與 高血 糖 白鼠에 미치는 迎香 高基德
  14. Science Streptozotocin-induced pancreatic insulitis new model of diabetes mellitus Like, A.A.;Rossini, A.A.
  15. Diabetes v.38 Increased ${\beta}$-cell secretory responsiveness to ceruletide and TPA in streptozotocin-induced mildly diabetic rats Okabayashi, Y.;Otsuki, M.;Ohki, A.;Tani, S.;Baba, S.
  16. J. Clin. Invest. v.48 Diabetogenic action of streptozotocin : Relationship of dose to metabolic reponse Junod, A.;Lambert, A.E.;Strauffacher, W.;Renold, A.E.
  17. Diabetes v.30 Responses of neonetal rat islecs to streptozotocin Bonner-weir, G.C.
  18. Diabetes v.27 no.12 Alloxan stimulation and inhibition of insulin release from isolated rat islets of langerhans Weaver, D.C.;Mcdaniel, M.L.;Naber, S.P.;Barry, D.;Lacy, P.E.
  19. Inhibition of intestinal disaccharidases and suppression of blood glucose by a new ${\alpha}$-glucohydrolase inhibitor-MDL v.25 Rhinehart, B.L.;Robinson, K.M.;Liu, P.S.;Payne, A.J.;Whetley, M.E.;Wagner, S.R.
  20. Brit. J. Pharmacol. v.22 Whittle, B.A.
  21. J. Pharm. v.24 Shimomura, K.
  22. J. Immunol. v.95 Golub, E.S.;Spitznagel, J.K.
  23. Yahhakhoeji v.31 no.3 Daidzen의 항염작용과 그 작용기전에 관한 연구 허인회';이상준;김영준
  24. Folia Pharmacol. Japan v.73 Hara, Y.;Tomizawa, S.
  25. Proc. Natl. Acad. Sci. USA v.75 Planning for lymphocytes : A method for cell selection Wysocki, L.J.;Sato, V.L.
  26. J. Immunol. v.120 Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1 Mizel, S.B.;Openheim, J.J.;Rosensteich, D.L.
  27. J. Immunol. methods. v.65 Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxic assays Mosmann, T.
  28. J. Immunol. methods. v.129 A simple and rapid method to determine hematopoietic growth factor activity Kotnic, V.;Fleischmann, W.R.Jr.
  29. Infec. Immunity v.59 no.9 Killing of Plasmodium faciparum in vitro by nitric oxide derivatives Rockett, K.A.;Awburn, M.M.;Cowden, W.B.;Clark, I.A.
  30. J. Immunol. Methods v.174 Chemiluminenscence and nitrite determinations by the MALU macrophage cell line Boudard, F.;Vallot, N.;Cabaner, C.;Bastide, M.
  31. J. Immunol. Methods v.112 Measurement of phagocyte chemilumi-nenscence in a microtiter plate format Blair, A.L.;Cree, I.A.;Beck, J.S.;Hating, M.J.G.
  32. J. Immuno. Methods v.60 Differentiation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay Sahlin, S.;Hed, J.;Rundquist, I.
  33. J. Immuno. Methods v.162 A rapid and simple micro-fluorometric phagocytosis assay Chok, P.W.;Choon, S.P.;Benjamin, H.S.
  34. 四象醫學原論 洪淳用;李乙浩
  35. 本草學 全國醫科大學 本草學敎室
  36. 內科學 李文鎬(등)
  37. Pharmacol. Rev. v.22 Drugs producing diabetes through damage of the insulin secreting cells Rerup, C.C.
  38. Renal. Physiol. v.7 The effect of streptozotocin and streptozotocin induced daibetes on the kidney Evan, A.P.;Mong, S.A.;Gattone, V.H.;Connors, B.A.;Aronoff, G.R.;Luft, F.C.
  39. Proceedings of the Society for Experimental Biology and Medicine v.61 Lazarow, A.
  40. Screening methods for drug evaluation(III) v.9 Saito, H.
  41. Cellular and molecular immunology. 2ed. Abbas, A.K.;Lichtman, A.H.;Pober, J.S.
  42. Reviews of infectious disease v.9 Human urinederived inhibitors of interleukin-1 Rosenstreich, D.L.;Yost, S.L.
  43. J. Rheumatology v.18 Chronic inflammatory joint diseases : natural inhibitors of interleukin-1 and tumor necrosis factor-${\alpha}$ Dayer, J.M.
  44. The cortical actin filament network of neutrophil leukocytes during phagocytosis and chemotaxis Sheterline, P.;Rickard, J.E.
  45. J. Cell Biol. v.110 no.5 Cytosolic free calcium elevation mediates the phagosomelysosome fusion during phagocytosis in human neutrophils Jaconi, M.E.E.;Lew, D.P.;Carpentier, J.L.;Magnusson, K.E.;Sjogren, M.;Stendahl, O.
  46. Biochem. and Biophysical Research Communications v.233 Nitric oxide-releasing particles inhibit phagocytosis in human neutrophils Forslund, T.;Sundqvist, T.
  47. J. Immunol. v.18 Nitric oxide inhibits macrophage pseudopodia formation in the activated macrophages Jun, C.D.;Park, S.K.;Kim, J.M.;Kim, J.D.;Chung, H.T.
  48. Eur. J. Pharmacol. v.211 Modulation of acute inflammation by endogenous nitric oxide Ialenti, A.;Ianaro, A.;Moncada, S.;Dirosa, M.
  49. Br. J. Pharmacol. v.110 Modulation of adjuvant arthritis by endogenous nitric oxide Ialenti, A.;Moncada, S.;Dirosa, M.
  50. Br. J. Exp. Pathol. v.65 Albumin inhibits human polymorphonuclear leukocyte luminol-dependent chemiluminescence : evidence for oxygen radical scavenging Holt, M.E.;Ryall, M.E.T.;Campbell, A.K.
  51. Infect. Immun. v.45 Intra-and extracellular events in luminol- dependent chemiluminescence of polymorphonuclear leukocytes Breiheim, G.;Stendahl, O.;Dahlgren, C.