Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Estimation of Cellular Damages Caused by Paraquat and lead Using a Cell Culture System

  • Park, Young-Im (Biotechnology Division, Korea Forest Research Institute) ;
  • Noh, Eun-Woon (Biotechnology Division, Korea Forest Research Institute) ;
  • Han, Mu-Seok (Biotechnology Division, Korea Forest Research Institute) ;
  • Yi, Yong-Sub (National instrumentation Center for Environmental Management, Seoul National University)
  • Published : 2001.05.01
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Abstract

A cell culture system of poplar (Populus alba x P.glandulosa) was established to test four different methods for evaluation of cellular stresses. Two different kinds of stresses were given to the cultures by adding either Pb(NO$_3$)$_2$ or paraquat and the cellular responses were monitored during a week period. While fresh weight reduction was observable in two days after the treatment of Pb(NO$_3$)$_2$, such changes were apparent only in later stage in paraquat treated cultures. Cells in paraquat treated cultures in the first 3 days showed no alteration in fresh weight as compared to untreated cultures, but had their MTT reducing activities completely inhibited. Neither Evans blue staining nor ion conductivity of the medium was consistent with fresh weight changes of the cultures. Overall, cell clumps formed during suspension culture appeared to interfere with staining and washing reactions and thus cause the assays unreliable. Among the four methods examined, fresh weight changes and MTT reducing activity appeared to be the most reliable and consistent.

Keywords

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