• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.192-195
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• 2003

As a part of our research project for in-situ bioremediation of nitrate contaminated. groundwater, screening studies to determine an effective electron donor (EO) and/or carbon source (CS) such as acetate, ethanol, formate, fumarate, lactate, and propionate were conducted. To evaluate the feasibility for the biological degradation of nitrate, soil microcosm studies using nitrate-contaminated soil and groundwater were performed. The nitrate removal percentage in the order from the highest to the lowest was: formate, fumarate, and ethanol > lactate > propionate. Essentially no nitrate consumption was observed In acetate-fed microcosms. The order of nitrate removal rate from the highest to lowest was fumarate, formate, lactate, ethanol, and propionate. These results suggest that fumarate and formate are promising EDs/CSs for in-situ bioremediation of nitrate - contaminated oxygenated groundwater.

• The Korean Journal of Zoology
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• v.32 no.4
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• pp.351-357
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• 1989

To investigate the effect of alcohol on the ultrastructural changes of liver the experiment was undertaken by dividing in 3 groups (control, alcohol and alcohol-camitine groups). Chronic administration of ethanol to adult rats led to striking fat accumulation and ultrastructural changes in the liver. Mitochondrial abnormalities. dilation of endoplasmic reticulum, focal cytoplasmic degradation, dilation of bile canalicli and swelling of nucleus were observed. In alcohol-carnitine group, there were less fat accumulation and ultrastructural changes than alcohol group. These alterations suggest that ethanol is very toxic to the liver and carnitine prevents fat accumulation induced by alcohol. 알콜이 흰쥐 간장의 미세구조에 미치는 영향을 관찰하기위해 대조군, 알콜 처리군, 알콜-carnitine처리군으로 나누어 실험하였다. 만성적인 알콜 투여는 간에서의 지방축적을 유발하였으며 미토콘드리아의 이상, 소포체의 팽대, 세포질의 손상 Bile canaliculi의 확장 및 핵의 수축 등의 미세구조적 변화를 가져왔다. 알롤- carnitine으로 처리한 군에서는 지방의 축적과 미세구조의 변화가 알콜 처리군에서 보다는 약하였다. 이러한 구조적 변화는 알콜이 간에 매우유독하며 carnitine은 알콜에 의해 유발된 지방의 축적을 방지해 보여주는 것으로 사료된다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.6 no.4
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• pp.337-341
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• 2005

The degradation of IPA according to coating conditions was examined by $TiO_2/P-25-coated$ POF. In the photoactivity of $TiO_2-coated$ POF, ethanol solvent was higher activity than other solvents. Inorganic(KR-400), organic(A-9540) and inorganic$\cdot$organic hybrid(GPTMS, TMOS) resins were used as binder. Organic binder(A-9540) showed the highest activity for degradation of IPA, but organic binder was decomposed by $TiO_2$. Inorganic binder had lower binder ability than others, and lower adhesive than organic binders. In TMOS as inorganic · organic hybrid binder, activity of IPA degradation was decreased by addition of TMOS when the ratio of TMOS and P-25 was changed from 0.05 to 1.

• Toxicological Research
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• v.33 no.2
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• pp.125-134
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• 2017

The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.

• Journal of Conservation Science
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• v.36 no.2
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• pp.103-111
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• 2020

To compare the cleaning effect of solvents such as water and ethanol used to clean lacquer relics, the components extracted with various solvents were analyzed. A freshly dried lacquer sample and a lacquer sample exposed to ultraviolet radiation for 24 days were treated with water, ethanol, acetone, and hexane, and the dissolved material was detected by pyrolysis-gas chromatography-mass spectrometry. The amount extracted was significantly higher in acetone and ethanol than in hexane, a nonpolar solvent. Water, a highly polar solvent, was relatively efficient for extracting low-molecular-weight materials. After experiencing 24 days of ultraviolet radiation, the lacquer exhibited a significant increase of extracted materials compared with the nonexposed one. This may be due to the degradation of the urushiol chain in the form of polymers, resulting in the formation of low-molecular-weight polar substances, including dicarboxylic acids. In addition to the deterioration status of lacquer relics, such solvent extraction properties will be a crucial consideration in selecting the appropriate cleaning solvent.

• Food Science of Animal Resources
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• v.22 no.3
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• pp.267-273
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• 2002

The functional properties of egg shell membrane hydrolysate by Bacillus licheniformis(EESMH) and NaOH-ethanol(AESMH) as a food material were investigated.. The yield of egg shell membrane hydrolysate was about 15% by Bacillus licheniformis, whereas that was 70% by NaOH-ethanol. Histidine content was higher in EESMH (18.69%) than in AESMH (2.56%). Both EESMH and AESMH showed high protein solubility (>95%). Emulsi-fying activity and stability of EESMH were higher than those of AESMH. foaming capacity and stability of AESMH were 2 times higher than those of EESMH in the pH ranges from 2 to 12. The AESMH had antioxidative activity whereas EESMH had not. Therefore, both AESMH and EESMH can be used for industrial food materials from the results of functional properties.

• Korean Journal of Plant Resources
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• v.30 no.6
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• pp.640-646
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• 2017

In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor $GSK3{\beta}$ but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through $GSK3{\beta}$-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.

• Journal of Korean Society on Water Environment
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• v.23 no.6
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• pp.961-965
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• 2007

In this study, degradation of carbohydrates and composition of fermentative products were investigated in semi-continuous acid fermenter varying hydraulic retention time (HRT). Rice soup was used as a sole substrate for the acid fermentation. Solubilization efficiency of the substrate was higher than 70% for all HRT, however the gas conversion was ignorable implying that most of organic contents in the influent remained in the form of volatile fatty acids (VFAs) and ethanol after acid fermentation. The VFAs were the predominant product and the VFAs conversion increased as the HRT decreased. The VFAs conversion reached the maximum value at 12 hr HRT accounting for 70% of the influent COD. Similar to VFAs, ethanol conversion was increased with the decrease of HRT and the maximum ethanol conversion efficiency was 8% at the HRT of 12 hr. Composition of VFAs was markedly dependent on HRT. As HRT increased, the composition of acetic acid was increased as a product of acetogenesis from butyrate, valerate and ethanol. This study demonstrated that HRT affected acid fermentation of a carbohydrate containing organic wastes producing VFAs and ethanol which could be effectively used to compensate the lack of carbon in wastewater for biological nutrient removal.

• Journal of Korean Medicine Rehabilitation
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• v.27 no.2
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• pp.1-8
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• 2017

Objectives Ethanol is a potent inhibitor of muscle protein synthesis. Muscle mass is regulated by the balance between rates of protein synthesis and protein breakdown. Both acute and chronic alcohol consumption inhibits synthesis to a greater extent than degradation. Protein synthesis is more intensely decreased in type II fibers than in type I fibers. Apoptosis has been shown to occur frequently in a variety of tissues in response to chronic alcohol feeding. Increased muscle fiber apoptosis has been shown in alcoholics with myopathy. Pueraria radix has been used for many disorders such as fevers, gastrointestinal disorders, muscle aches, allergies, respiratory problems, skin problems, high blood pressure, migraine headaches, lowering cholesterol and treating chronic alcoholism. We therefore tested the hypothesis that oral treatment with Pueraria radix could reduce the ethanol-induced muscle atrophy. Methods Young male Sprague-Dawley rats were orally given 25% ethanol (5 ml/kg, body weight) daily with Ethanol for 4 weeks. Normal group was similarly administrated with saline. The Rats of Pueraria radix treated group (EtOH+PR) were orally administrated Pueraria radix water extract, and rats of EtOH group were given with the vehicle only. After 4 week, the morphology of gastrocnemius and plantaris muscles were assessed by hematoxylin and eosin staining. The immunoreactivities of pre-apoptotic BAX and anti-apoptotic Bcl-2 proteins were also measured. Results The muscles from rats of EtOH group represented a significant reduction in average cross section area compared to Normal group. EtOH+PR group had increased fiber compared to the EtOH group. Moreover, to investigate the ethanol-induced muscular apoptosis, the immunohistochemical analysis of Bax and Bcl-2 was carried out. The treatment with Pueraria radix (EtOH+PR) significantly decreased BAX expression and increased Bcl-2 expression 4 weeks after ethanol administration when compared with Normal group. Conclusions These results suggest that Pueraria radix water extract has protective effects on chronic alcohol induced myopathy.

• Journal of the Korean Wood Science and Technology
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• v.47 no.2
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• pp.145-162
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• 2019

This study aimed to increase the sugar and ethanol yield from the mengkuang plant biomass through biological and liquid hot water (LHW) pretreatment and their combination. The results showed that biological pretreatments with 5% inoculum of the fungus Trametes versicolor resulted in the highest alpha cellulose content incubated for 30 days, and 10% inoculum resulted in the lowest lignin content. LHW pretreatment decreased the hemicellulose content of pulps from 10.17% to 9.99%. T. versicolor altered the structure of the mengkuang pulp by degrading the lignin and lignocellulose matrix. The resulting delignification and cellulose degradation facilitate the hydrolysis of cellulose into sugars. The alpha cellulose content after biological-LHW pretreatment was higher (78.99%) compared to LHW-biological pretreatment (76.85%). Scanning electron microscopy analysis showed that biological-LHW combinated treatment degrades the cell wall structures. The ethanol yield for biological-LHW pretreated sample was observed 43.86% (13.11 g/L ethanol by weight of the substrate, which is much higher than that of LHW-biological pretreatment (34.02%; 9.097 g/L). The highest reducing sugar content about 45.10% was observed with a resulting ethanol content of 15.5 g/L at LHW pretreatment temperature of $180^{\circ}C$ for 30 min.