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http://dx.doi.org/10.7732/kjpr.2017.30.6.640

Anti-Proliferative Activity of Ethanol Extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) Through Cyclin D1 Proteasomal Degradation in Human Colorectal Cancer Cells  

Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
Song, Hun Min (Department of Medicinal Plant Resources, Andong National University)
Park, Su Bin (Department of Medicinal Plant Resources, Andong National University)
Park, Ji Hye (Department of Medicinal Plant Resources, Andong National University)
Shin, Myeong Su (Department of Medicinal Plant Resources, Andong National University)
Son, Ho-Jun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
Um, Yurry (Forest Medicinal Resources Research Center, National Institute of Forest Science)
Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.30, no.6, 2017 , pp. 640-646 More about this Journal
Abstract
In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor $GSK3{\beta}$ but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through $GSK3{\beta}$-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.
Keywords
Anticancer activity; Cancer chemoprevention; Cyclin D1; Human colorectal cancer; Taxilli Ramulus; Taxillus chinensis (DC.) Danser;
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