• The Plant Pathology Journal
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• v.38 no.2
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• pp.115-130
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• 2022

Though information exists regarding the pathogenesis of the shot-hole disease (SH) in flowering cherry (FC), there has been a lack of research focusing on SH management. Therefore, here, we investigated the inhibitory activities of antagonistic bacteria against SH pathogens both in vitro and in vivo as well as their biochemical characteristics and bioactive compounds. Two biosurfactant-producing bacterial antagonists, identified as Bacillus velezensis strains JCK-1618 and JCK-1696, exhibited the best effects against the growth of both bacterial and fungal SH pathogens in vitro through their cell-free culture filtrates (CFCFs). These two strains also strongly inhibited the growth of the pathogens via the action of their antimicrobial diffusible compounds and antimicrobial volatile organic compounds (VOCs). Crude enzymes, solvent extracts, and biosurfactants of the two strains exhibited antimicrobial activities. Liquid chromatography/electrospray ionization time-of-flight mass spectrometric analysis of the partially purified active fractions revealed that the two antagonists produced three cyclic lipopeptides, including iturin A, fengycin A, and surfactin, and a polyketide, oxydifficidin. In a detached leaf assay, pre-treatment and co-treatment of FC leaves with the CFCFs led to a large reduction in the severity of the leaf spots caused by Epicoccum tobaicum and Bukholderia contaminans, respectively. In addition, the two antagonists produced indole-3-acetic acid, siderophore, and a series of hydrolytic enzymes, along with the formation of a substantial biofilm. To our knowledge, this is the first report of the antimicrobial activities of the diffusible compounds and VOCs of B. velezensis against the SH pathogens and their efficiency in the biocontrol of SH.

• Korean Journal of Agricultural Science
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• v.38 no.3
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• pp.413-419
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• 2011

Recently, the rate of cultivation of such as hairy vetch was increased as a way of reducing the current soil problem. However, the occurrence of insect pest such as bean bugs, Riptortus pedestris, were increased in the field of green manure crops and their injuries to hairy vetch were observed. While minimizing insecticide use for the environmental friendly agriculture, the control of the bean bug can be utilized environmental friendly agricultural materials (EFAMs) in the green manure crop fields. The control effects of some EFAMs and most of chemical insecticides to the bean bug were significantly high. As a result of direct spray of EFAM that contained sophora extract or neem extract, the control effects of six EFAMs were higher than 70% at 120 hours after treatments. Among them, three EFAMs were showed over 90% of control effects. On the other hand, most of chemical insecticides were showed 100% of control effect against the bean bug at 48 hours after treatment. As mortality effects of EFAMs were sloely observed until 120h after treatment, we must use selected EFAMs at the beginnibgs of occurrence and entrance in the field of green manure for effective control of bean bugs.

• The Plant Pathology Journal
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• v.33 no.5
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• pp.499-507
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• 2017

In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of $31.3{\mu}g/ml$ and 100% at a concentration of $250{\mu}g/ml$. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.2
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• pp.63-72
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• 2011

The aim of this study, a traditional residential area in the environmentally friendly design techniques to identify the techniques and principles that have been carried out to reestablish the principles. To do this, through literature review environmental performance is reflected in the traditional residential area side of resources conservation, locational aspects, spatial configuration, and how cases were selected looking for ways to apply modern. Are examples of upper class housing in the Chosen Dynasty Period period construction relatively well-preserved round and a good building with a clear housing Dokrakdang year were selected. Locational aspects of the terrain with minimal changes to the building and construction techniques were entirely in terms of environmental conservation and environmental temperature was adjusted to regulate the room temperature technique could be seen. In terms of cycling in natural materials were recycled. and water make used of positive through water cycling technique & water control. In addition, the importance of landscape views overlooking the landscape from inside to outside through the regulation of the various internal and external space technique was used to attract and expand. Traditionality in the pursuit of modern space, simply cut off because of tradition rather than to restore or recover the organizing principle inherent in the traditional space, and extraction of the contemporary social, cultural and environmental understanding of space is acceptable in basis. Environmentally-friendly design techniques in a traditional residential area for a long time to be developed by the experience of its application of modern environmental and energy problems and pleasant environment to the creation of human life and are subject to significant swings in that.

• Korean Journal of Organic Agriculture
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• v.15 no.1
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• pp.25-42
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• 2007

Hansalim is a cooperative organization dealing with environmental-friendly/organic products(EFOP), which is pursuing both movement and business. Hansalim consists of producer's organizations and consumer's organizations, and always talks over organization structures and management directions all together. Hansalim has the movement goal of values and a world view for all lives, and so produces, distributes and consumes the EFOP as a way of achieving the goal. Hansalim was founded in 1986, and has about 130,000 members, the total sales of about 93.6 billions, 19 regional hansalims and one logistics center in 2006. Product strategy and promotion strategy are remarkable among green marketing mix of hansalim. Product strategy focuses safety, the environmental intimacy, differentiation and superiority of products. And the characteristics of promotion strategy are spontaneity, the self-control, cooperative spirit, mutual trust and close relationship among producers, consumers and staffs in charge.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.35-43
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• 2018

Root-knot nematodes (Meloidogyne spp.) are parasites that attack many field crops and orchard trees, and affect both the quantity and quality of the products. A root-colonizing bacterium, Pseudomonas chlororaphis O6, possesses beneficial traits including strong nematicidal activity. To determine the molecular mechanisms involved in the nematicidal activity of P. chlororaphis O6, we constructed two mutants; one lacking hydrogen cyanide production, and a second lacking an insecticidal toxin, FitD. Root drenching with wild-type P. chlororaphis O6 cells caused juvenile mortality in vitro and in planta. Efficacy was not altered in the fitD mutant compared to the wild-type but was reduced in both bioassays for the mutant lacking hydrogen cyanide production. The reduced number of galls on tomato plants caused by the wild-type strain was comparable to that of a standard chemical nematicide. These findings suggest that hydrogen cyanide-producing root colonizers, such as P. chlororaphis O6, could be formulated as "green" nematicides that are compatible with many crops and offer agricultural sustainability.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.119.1-119.1
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• 2014

Toxic waste disposal: Many people think that when toxic waste is dumped into the ocean or into the air, it disappears. This belief is incorrect. Rather than disappearing, it accumulates over time and slowly destroys the environment. Ultimately, it leads to the destruction of human race. Plasma is environmentally friendly: Plasma is environmentally friendly because it is created and disappears. When plasma is formed on the earth, you need certain conditions such as accelerating electrons by an electrical discharge or a particle accelerator. When this is gone, plasma completely disappears, leaving no impact on the environment. Plasmas produce radicals: Even if plasma density is low at atmospheric pressure, many radicals (excited states of molecules) are created. These radicals are chemically very aggressive. So instead of using harmful chemicals, plasma can be utilized for less of an impact on the environment. Plasma can reach very high temperatures: Plasma is also useful because when you control the density, you can easily reach high temperatures up to $5000{\sim}6000^{\circ}C$ at atmosphere pressure. Because of this heat and the chemical aggressiveness of the plasma, there are many green applications for plasma technology. Pulsed power technology: Pulsed electric field for extraction, drying and killing bacteria. Treatment of biological tissue by pulsed electric fields: Extraction of substances from cells: Sterilisation, Medical applications, Growth stimulation, Food preparation. Each application has its specialities, especially with respect to pulse shape and electric field strength.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2001.05a
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• pp.117-120
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• 2001

This study is a preliminary research to develop design principles for environmentally friendly housing. The purposes of study are to investigate the literatures related passive design for summer and theory of ventilation, to analyze the indoor airflow patterns in traditional Korean house during summer, and to propose the design factors for effective passive cooling system. The analysis for airflow patterns was focused on the ‘An bang’and the ‘Dae Chung’in the ‘An Chae’of a traditional house located in Seoul. Field measurements of air temperature and air velocity were carried out at 30 different measuring points with 8 different window-opening conditions. The measurements were taken on the hottest summer days in August 2000. It is concluded that from an environmentally friendly standpoint design factors to control indoor thermal environment by a passive cooling system during the summer are as follows; ceiling structure has thermal performance like a time-lag effect, optimum height and length of eaves which can prevent sunlight and divert airflow toward the sitting level, building arrangement acceptable the prevailing wind, strategic window arrangement which makes cross ventilation possible (especially north-south) at the sitting level, window opening condition which is possible to intersect two cross-ventilation stream at the main living areas, northward windows remaining in shade to create the air pressure difference, and planning building shape like a bracket that has optimum width and depth.

• Korean Journal of Environmental Agriculture
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• v.29 no.3
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• pp.239-246
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• 2010

Aphids are one of the major pests in agricultural crops. A number of synthetic pesticides have been used for control of aphids in agriculture, but increasing public concerns over their adverse effects on the environment have required more environmentally-friendly methods for pest management. In this study, we examined plant oil formulations for the control of green peach aphid (Myzus persicae). Oil formulations were prepared by hydrolyzing the plant oils in ethanolic KOH solution and diluted at the rate of 1:500 for aphid control. The oil formulations showed aphid mortalities ranging from 24.44 to 43.33% in vitro. Significantly increased aphid mortalities were observed by the treatment of oil formulations combined with low-dosed imidacloprid. No significant difference in the aphid mortality was observed between the oil formulations. Mass spectrometry analyses of aphids treated with the low dosedimidacloprid plus the plant oil formulations detected similar concentrations of imidacloprid between the treatments. In field trial bioassays against aphids, significantly decreased aphid population were observed in the pepper plants treated with soybean oil formulation combined with the low-dosed imidacloprid, while aphid population dramatically increased in the pepper plants treated with the low-dosed imidacloprid alone. These results suggested that the plant oil formulations can be used as an environmentally-friendly method for enhancing the insecticidal effectiveness, which may play a role in reducing the use of synthetic pesticide in agriculture.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.2
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• pp.188-194
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• 2006

Since the late 1920's possibility of commercial hydroponics was testified practically. Hydroponics is used as environmentally friendly agriculture production system recently with high effectiveness. Now that existing irrigation control systems such as time control or solar radiation control cannot satisfy stable water content in root substrates, the needs for new irrigation system keep increasing. In this paper, we proposed environmentally friendly automatic irrigation management system by employing automation system based on electronic control system, which could solve problems based on manual irrigation management system. In addition, it suggested to be applied to any crops and will be able to overcome existing limit in irrigation by measuring the water content of root substrate in realtime.