• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.5
• /
• pp.485-491
• /
• 2015

This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of $60^{\circ}C$ for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.

• Korean journal of applied entomology
• /
• v.60 no.4
• /
• pp.417-422
• /
• 2021

This study aimed to provide information on the biodiversity of various arthropods living in paddy fields during the winter season by examining their incidence density. Insect collection was carried out by 50 × 50 cm grids of soil surface and plants from paddy fields, as well as from ridges between paddies, using a battery-powered aspirator. The total number of individuals collected from paddy fields and inter-paddy ridges was 41,197, most of belonged to the orders Collembola, Hemiptera, and Diptera. Arthropods showed similar appearance patterns in fields under both organic and conventional farming. However, a higher number of insects was collected in organic farms. The species diversity, species richness, and species evenness were all greater in inter-paddy ridges than in paddy fields, in a statistically significant manner (P < 0.05).

• Journal of the Korean GEO-environmental Society
• /
• v.12 no.11
• /
• pp.13-21
• /
• 2011

To recover basic functions of river such as water control, irrigation, environment, culture, a national river improvement project, the four river restoration projects were currently planned and under construction in Korea. This project is designed to preserve cultural assets and ecosystem from flooding, for that reason, environmentally friendly materials of construction are strongly emphasized. In this study, the soil and cement admixtures are developed. And, the compaction test and the unconfined compressive strength test to evaluate applicability of probiotics as environmentally friendly materials are conducted the soil and cement admixtures. As a result, the probiotic culture was not active in completely dried specimen to obtain accurate mixing proportion. It indicates that the probiotics cannot influence on the development the soil and cement admixtures. A further research will focus on the effect of response between polysaccharide environmentally friendly soil stabilizer and natural specimen.

• The Plant Pathology Journal
• /
• v.38 no.3
• /
• pp.182-193
• /
• 2022

Turfgrass, the most widely grown ornamental crop, is severely affected by fungal pathogens including Sclerotinia homoeocarpa, Rhizoctonia solani, and Magnaporthe poae. At present, turfgrass fungal disease management predominantly relies on synthetic fungicide treatments. However, the extensive application of fungicides to the soil increases residual detection frequency, raising concerns for the environment and human health. The bacterial volatile compound, 2,3-butanediol (BDO), was found to induce plant resistance. In this study, we evaluated the disease control efficacy of a combination of stereoisomers of 2,3-BDO and commercial fungicides against turfgrass fungal diseases in both growth room and fields. In the growth room experiment, the combination of 0.9% 2R,3R-BDO (levo) soluble liquid (SL) formulation and 9% 2R,3S-BDO (meso) SL with half concentration of fungicides significantly increased the disease control efficacy against dollar spot and summer patch disease when compared to the half concentration of fungicide alone. In field experiments, the disease control efficiency of levo 0.9% and meso 9% SL, in combination with a fungicide, was confirmed against dollar spot and large patch disease. Additionally, the induction of defense-related genes involved in the salicylic acid and jasmonic acid/ethylene signaling pathways and reactive oxygen species detoxification-related genes under Clarireedia sp. infection was confirmed with levo 0.9% and meso 9% SL treatment in creeping bentgrass. Our findings suggest that 2,3-BDO isomer formulations can be combined with chemical fungicides as a new integrated tool to control Clarireedia sp. infection in turfgrass, thereby reducing the use of chemical fungicides.

• The Plant Pathology Journal
• /
• v.35 no.6
• /
• pp.662-673
• /
• 2019

Root-knot nematodes (RKNs) are among the most destructive plant-parasites worldwide, and RKN control has been attempted mainly using chemical nematicides. However, these chemical nematicides have negative effects on humans and the environment, thus necessitating the search for eco-friendly alternative RKN control methods. Here, we screened nematicidal lactic acid bacteria (LAB) isolated from kimchi and evaluated their efficacy as biocontrol agents against RKNs. Of 237 bacterial strains, Lactobacillus brevis WiKim0069 showed the strongest nematicidal activity against the second-stage juveniles (J2) of Meloidogyne incognita, M. arenaria, and M. hapla and inhibited the egg hatch of M. incognita. The culture filtrate of WiKim0069 had a pH of 4.2 and contained acetic acid (11,190 ㎍/ml), lactic acid (7,790 ㎍/ml), malic acid (470 ㎍/ml), and succinic acid (660 ㎍/ml). An artificial mixture of the four organic acids produced by WiKim0069 also induced 98% M. incognita J2 mortality at a concentration of 1.25%, indicating that its nematicidal activity was derived mainly from the four organic acids. Application of WiKim0069 culture filtrate suppressed the formation of galls and egg masses on tomato roots by M. incognita in a dose-dependent manner in a pot experiment. The fermentation broth of WiKim0069 also reduced gall formation on melon under field conditions, with a higher efficacy (62.8%) than that of fosthiazate (32.8%). This study is the first report to identify the effectiveness of kimchi LAB against RKNs and to demonstrate that the organic acids produced by LAB can be used for the RKN management.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.10
• /
• pp.1567-1582
• /
• 2015

The purpose of this study was to examine the status of environmentally-friendly ingredients and dietitians' perceptions toward them in order to find for improvement. Data was collected from questionnaires completed by 202 school dietitians and nutrition teachers in Chungbuk, and this data was analyzed utilizing the SPSS 20.0 program. The results obtained from this study were as follows: 'mixed grains (58.9%)' and 'eggs (36.6%)' were found to be the most used environmentally-friendly ingredients. 81.7% of the respondents said they 'never used' marine products, whereas 'fruits (43.6%)', 'pork (40.8%)', and 'fish (54.5%)' were the most preferred ingredients. Dietitians and nutrition teachers used environmentally-friendly marine products less than other ingredients, had a poor understanding about environmentally-friendly marine products, and demonstrated low reliability and belief in the necessity of the system. In order to verify the environmentally-friendly status of the ingredients, marks on the product and documents of certification were mostly used. In order to improve the supply system, a more strict tracking system in the distribution process by securing more reliable suppliers is required.

• 한국균학회소식:학술대회논문집
• /
• 2018.05a
• /
• pp.44-44
• /
• 2018

Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB. B. amyloliquefaciens JCK-12 produces several cyclic lipopeptides (CLPs) including iturin A, fengycin, and surfactin. Iturin A inhibits spore germination of F. graminearum. Fengycin or surfactin alone did not display any inhibitory activity against spore germination at concentrations less than 30 ug/ml, but a mixture of iturin A, fengycin, and surfactin showed a remarkable synergistic inhibitory effect on F. graminearum spore germination. The fermentation broth and formulation of B. amyloliquefaciens JCK-12 strain reduced the disease incidence of FHB in wheat. Furthermore, co-application of B. amyloliquefaciens JCK-12 and chemical fungicides resulted in synergistic in vitro antifungal effects and significant disease control efficacy against FHB under greenhouse and field conditions, suggesting that B. amyloliquefaciens JCK-12 has a strong chemosensitizing effect. The synergistic antifungal effect of B. amyloliquefaciens JCK-12 and chemical fungicides in combination may result from the cell wall damage and altered cell membrane permeability in the phytopathogenic fungi caused by the CLP mixtures and subsequent increased sensitivity of F. graminearum to fungicides. In addition, B. amyloliquefaciens JCK-12 showed the potential to reduce trichothecenes mycotoxin production. The results of this study indicate that B. amyloliquefaciens JCK-12 could be used as an available biocontrol agent or as a chemosensitizer to chemical fungicides for controlling FHB disease and as a strategy for preventing the contamination of harvested crops with mycotoxins.

• Proceedings of the KSR Conference
• /
• 2011.10a
• /
• pp.3237-3250
• /
• 2011

As the growing concern about environment and earthquake for the concrete structure, many seismic rehabilitation and retrofitting methods have recently been studied but they are not coping enough with the changes of structure, specificly various problems have been found in seismic rehabilitation method - both in exposure or non exposure - when they are implemented to the underground structure, utility conduit, water supply facilities, underground wall, parking lot, road pavement, and elevated structure etc. This study is about the seismic rehabilitation method using environmentally friendly functional inorganic mortar and resilient material, and it is effectively retrofitted seismic performance as it reinforces not only physical strength, but also flexural and bond strength from the resilient material, and it has been analyzed and evaluated when the environmentally friendly functional inorganic mortar and the resilient material are applied so as to countermeasure the effect of earthquake and viable problems and approved for possibility of various applications and wide use.

• Mycobiology
• /
• v.46 no.2
• /
• pp.154-158
• /
• 2018

Bitter rot caused by the fungal genus Colletotrichum is a well-known, common disease of apple and causes significant yield loss. In 2013, six fungal strains were isolated from Fuji apple fruits exhibiting symptoms of bitter rot from Andong, Korea. These strains were identified as Colletotrichum fructicola and C. siamense based on morphological characteristics and multilocus sequence analysis of the internal transcribed spacer rDNA, actin, calmodulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase Pathogenicity tests confirmed the involvement of C. fructicola and C. siamense in the development of disease symptoms on apple fruits. This is the first report of C. fructicola and C. siamense causing bitter rot on apple fruit in Korea.

• Korean Journal of Organic Agriculture
• /
• v.12 no.1
• /
• pp.67-80
• /
• 2004

Farm economy is getting worse because of the free trade trends in agriculture sector and unreasonable application of market economy principles to agricultural products. And The worldwide issues of environmental pollution as well as want of food, water and energy make human future be anxious. In addition, consumers raise questions in argument about safety of foods produced by traditional farming or imported. So I propose the urban-rural living community movement(URLCM) as an alternative plan for sustainable domestic agriculture. URLCM based on environmentally friendly agriculture and community consciousness will be able to solve many of present issues on agriculture and rural society through incessant interchanges between urban and rural community. This URLCM has to be basically founded on principles of symbiosis-coexistence and life circulation. So those that have to be driven forward arc organizing producers and consumer cooperatives, making environmentally friendly rural region and products, combining with consumer cooperatives, training the participants and so forth.