• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2002.04a
• /
• pp.49-58
• /
• 2002

Oxidative stress derived from reactive oxygen species (ROS) is one of the major damaging factors in plants exposed to environmental stress. In order to develop the platform technology to solve the global food and environmental problems in the 21s1 century, we focus on the understanding of the antioxidative mechanism in plant cells, the development of oxidative stress-inducible antioxidant genes, and the development of transgenic plants with enhanced tolerance to stress. In this report, we describe our recent results on industrial transgenic plants by the gene manipulation of antioxidant enzymes. Transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts were developed and were evaluated their protection effects against stresses, suggesting that simultaneous overexpression of both SOD and APX in chloroplasts has synergistic effects to overcome the oxidative stress under unfavorable environments. Transgenic tobacco plants expressing a human dehydroascorbate reductase gene in chloroplasts were showed the protection against the oxidative stress in plants. Transgenic cucumber plants expressing high level of SOD in fruits were successfully generated to use the functional cosmetic purpose as a plant bioreactor. In addition, we developed a strong oxidative stress-inducible peroxidase promoter, SWPA2 from sweetpotato (Ipomoea batatas). We anticipate that SWPA2 promoter will be biotechnologically useful for the development of transgenic plants with enhanced tolerance to environmental stress and particularly transgenic cell lines engineered to produce key pharmaceutical proteins.

• Journal of Environmental Health Sciences
• /
• v.44 no.5
• /
• pp.502-513
• /
• 2018

Objectives: Stress hormone (cortisol) can be used to analyze psychological and physiological changes and healing effects before and after in blood and saliva. Here it is applied to scientifically validate the effects of the healthy forest experience in Deogyusan Mountain National Park. Methods: A healthy forest experience program was provided to college students (males and females) at Deogyusan National Park. Stress, hormones (cortisol), blood pressure, pulse, oxygen saturation and stress, anxiety, and depression were measured. This study was designed to objectively analyze the effects of stress hormones before and after. Results: Depression, stress, anxiety, and satisfaction with the forest experience program in Deogyusan National Park were very high in reliability in terms of Cronbach's Alpha: depression scored 0.910, stress 0.558, anxiety 0.934, program satisfaction 0.826, and program effectiveness 0.894. Conclusions: In this study, we utilized the Healthy Forest Experience Program and the 'Health and Medical Center Health Record Book' of the Health Forest Experience Program (Ministry of Environment's Environmental Education Certification Program). In the future, we will seek ways to utilize saliva since it is easier to collect than blood samples and apply the stress hormone cortisol as a saliva authentication program. In addition, by implementing ecological welfare programs in which nature and human beings coexist through systematic preservation and restoration, the national park management can utilize ecological, scenic and cultural services wisely. We expect to be able to provide exploration and welfare services.

• Journal of the Korean Home Economics Association
• /
• v.35 no.2
• /
• pp.33-47
• /
• 1997

The purpose of the study is to examine the differences between rural and urban in the factors and the level of stress in adolescent, inner control and communication with parents. Also, It is to research the influence of environmental variables, inner control and communication with parents on adolescent's stress in sides of rural and urban. Therefore, I provide needed data in instructing students. For the data set, academic high school students in second grade in a town for rural and Su-Seung gu, Dal-Sugu, chung gu in Dae-gu for urban were chosen. The data were analyzed with the frequency, percentage, factor analysis Mean, S.D, reliability, t-test, regression in use of Spss PC+ Program. The main results are as follow. First, for rural students, stress level is displayed studies, future0affair, life-attitude, companions, self-assurance and home life in order by lower sphere. For urban students, it is displayed future-affair, studies, life-attitude, companions, self-assurance and home life in order by lower shpere. In case of rural "I don't know effective study method" is highly displayed by factors. In case of urban. "I am tired of a competitive life" is highly displayed. The mean of stress level is 3.22 for rural and 3.07 for urban. The differences between rural and urban are showed in self-assurance, studies and companions. Finally when we see the stress generally the stress level is higher in rural high school students than in urban high school students. Second, rural students are lower displayed than urban students in inner control and communication with parents. There is no difference urban students in inner control and communication with parents. There is no difference in communication with a mother. third, influential variables on stress level are inner control, communication with parents, satisfaction with companions and school life as environmental variables in sides of rural and urban. Physical condition is added for rural and sex for urban Finally we get the result that stress level is high in low inner control, poor communication with parents, bad companions and unsatisfied school life for both rural and urban. Bad physical condition for rural and female for urban are added.

• Journal of Korean Medical Ki-Gong Academy
• /
• v.13 no.1
• /
• pp.105-144
• /
• 2013

This study intended to provide fundamental data to take countermeasures against recurrent stress by analyzing the health-related quality of life and factors for recurrent stress of alcohol dependents. Structured questionnaire was prepared for patients who quitted drinking after they had been hospitalized for alcohol dependence to take medical treatment but suffered recurrence, and analysis on health-related quality of life and environmental factors with drinking risk was conducted. The quality of life was at a relatively satisfactory level, where pain was found at the highest level and general health condition was found at the lowest level. Environmental factors with drinking risk were found to be a moderate level, drinking for the reason of family and friends in terms of complications with other persons was found to be highest, and then job, self control, bad emotions, and social pressure on drinking were found in order. For health-related quality of life, the quality of life was found to be high in case of the only son, professional job, well-educated persons, and no history of hospitalization. Regarding factors for recurrent stress, stress was found to be high in case of the eldest son and being without religion, and the lower was the quality of life, the higher were recurrent stress and environmental factors with drinking risk. It was found that demographic factors including age, marriage, and job influenced the recurrence of alcohol dependence, and factors for recurrent stress, etc. also influenced recurrent alcohol dependence with drinking risks. Specially, the lower was the quality of life, the higher were factors for recurrent stress, and drinking risks, therefore measures to improve the quality of life are required to be taken to prevent alcohol dependence from recurrence.

• International Journal of Highway Engineering
• /
• v.10 no.4
• /
• pp.269-279
• /
• 2008

This study was conducted to develop the design methodology of transverse post-tensioning for the prestressed concrete pavement (PSCP). The transverse stress distribution was analyzed when the transverse anchor spacing changed. The tensile stress distribution in the PSCP slab due to the environmental and vehicle loads was also investigated. The reasonable methods were discussed to determine the design loads including environmental and vehicle loads and the PSCP allowable tensile stress used for the basis of the selection of the stress application amount from the tensioning. The results of this study showed that as the transverse anchor spacing increased, the range of the stress loss became larger and the stress loss was significant near the shoulder. The design of the transverse post-tensioning can be performed by obtaining the stresses under the design loads and by considering the allowable tensile stress; however, the tensile stresses at different locations such as the shoulder, wheel pass, and slab interior should also be checked and kept below the allowable tensile stress.

• Safety and Health at Work
• /
• v.14 no.4
• /
• pp.425-430
• /
• 2023

Background: It is acknowledged that legislation acts as a motivator for organizational action on psychosocial risks. Our study aims to provide evidence on the relationship between key occupational safety and health (OSH) policy principles and organizational action on work-related stress, and, in turn, with reported employee job demands and resources and their experience of work-related stress. We focus on Italy where specific legislation and practices on work-related stress were introduced in 2008 which are underpinned by these key OSH policy principles. Methods: Secondary analysis of the Italian samples from the employer ESENER-2 and employee 6th EWCS surveys was conducted, using path analysis in structural equation modeling (SEM) linking the two datasets. Results: We found a strong statistically significant relationship between OSH policy principles and organizational action on work-related stress (C.I. = .62-.78 p < .001). The existence of an organizational action plan on work-related stress was found to be significantly associated with more reported job resources (C.I. = .02-.24, p < .05) but these were not found to be significantly associated with less work-related stress. No significant association was found between having an organizational action plan for work-related stress and reported job demands. However, job demands were significantly related to reported work-related stress (C.I. = .27-.47, p < .001). Conclusions: Findings add support to the call for specific legislation on work-related psychosocial risks and highlight how an organizational OSH culture underpinned by key OSH principles, and awareness/competence development on psychosocial risk management can have a positive effect on organizational action. However, further support needs to be provided to organizations around developing primary prevention interventions at the organizational level with the aim of reducing job demands.

• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 1991.05a
• /
• pp.2-2
• /
• 1991

• Journal of Microbiology and Biotechnology
• /
• v.17 no.8
• /
• pp.1390-1393
• /
• 2007

In this study, three of the representative EDCs, $17{\beta}$-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas $17{\beta}$-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.9
• /
• pp.1288-1294
• /
• 2010

Rhizobium phaseoli strains were isolated from the mung bean (Vigna radiata L.) nodules, and the most salt tolerant and high auxin producing rhizobial isolate N20 was evaluated in the presence and absence of L-tryptophan (L-TRP) for improving the growth and yield of mung bean under saline conditions in a pot experiment. Mung bean seeds were inoculated with peat-based inoculum and NP fertilizers were applied at 30-60 kg/ha, respectively. Results revealed that imposition of salinity reduced the growth and yield of mung bean. On the contrary, the separate application of L-TRP and Rhizobium appeared to mitigate the adverse effects of salt stress. However, their combined application produced more pronounced effects and increased the plant height (28.2%), number of nodules per plant (71.4%), plant biomass (61.2%), grain yield (65.3%), and grain nitrogen concentration (22.4%) compared with untreated control. The growth promotion effect might be due to higher auxin production in the rhizosphere and improved mineral uptake that reduced the adverse effects of salinity. The results imply that supplementing Rhizobium inoculation with L-TRP could be a useful approach for improving the growth and yield of mung bean under salt stress conditions.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.3
• /
• pp.579-586
• /
• 2010

A genomic DNA fragment encoding a putative maltooligosyltrehalose synthase (NfMTS) for trehalose biosynthesis was cloned by the degenerate primer-PCR from cyanobacterium Nostoc flagelliforme. The ORF of NfMTS was 2,799 bp in length and encoded 933 amino acid residues constituting a 106.6 kDa protein. The deduced amino acid sequence of NfMTS contained 4 regions highly conserved for MTSs. By expression of NfMTS in E. coli, it was demonstrated that the recombinant protein catalyzed the conversion of maltohexaose to maltooligosyl trehalose. The $K_m$ of the recombinant enzyme for maltohexaose was 1.87 mM and the optimal temperature and pH of the recombinant enzyme was at $50^{\circ}C$ and 7.0, respectively. The expression of MTS of N. flagelliforme was upregulated, and both trehalose and sucrose contents increased significantly in N. flagelliforme during drought stress. However, trehalose accumulated in small quantities (about 0.36 mg/g DW), whereas sucrose accumulated in high quantities (about 0.90 mg/g DW), indicating both trehalose and sucrose were involved in dehydration stress response in N. flagelliforme and sucrose might act as a chemical chaperone rather than trehalose did during dehydration stress.