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Analysis of the Stress Effects of Endocrine Disrupting Chemicals (EDCs) on Escherichia coli  

Kim, Yeon-Seok (College of Life Sciences and Biotechnology, Korea University)
Min, Ji-Ho (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST))
Hong, Han-Na (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST))
Park, Ji-Hyun (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST))
Park, Kyeong-Seo (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST))
Gu, Man-Bock (College of Life Sciences and Biotechnology, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.8, 2007 , pp. 1390-1393 More about this Journal
Abstract
In this study, three of the representative EDCs, $17{\beta}$-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas $17{\beta}$-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.
Keywords
Stress effect; endocrine disrupting chemicals (EDCs); real-time RT-PCR; stress-specific responsive gene; gene expression analysis;
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Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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