• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.121-127
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• 2004

Amphotericin B (AmB), an amphipathic polyene macrolide, is an antifungal drug produced by Streptomyces nodosus. Recently, AmB has been shown to exert antiviral activity against rubella virus and human immunodeficiency virus by different mechanisms. In this study, we evaluated the antiviral effect of AmB against Japanese encephalitis virus (JEV) and investigated which step of the viral life cycle was inhibited by AmB to understand the mechanism of antiviral action of AmB. AmB reduced both plaque size and number in the infected cells in a dose-dependent manner. In addition, a 200-fold reduction of infectious virus titer was observed by treatment of infected cells with $5\mug/ml$ of AmB. AmB acted at the post virus-infection step, but not during adsorption of virus to host cells. Western blot analysis revealed that the accumulated level of JEV envelope protein dramatically decreased in the infected cells by treatment with $5-10\mug/ml$ of AmB. Our results indicate that AmB inhibits the replication of JEV at the postinfection step by interfering with viral replication and/or by inhibiting the synthesis of viral proteins.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1884-1889
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• 2008

The ${\beta}$-lactamase inhibitory protein, BLIP-II, found in the culture supernatant of Streptomyces exfoliatus SMF19, shows no discernible sequence identity with other ${\beta}$-lactamase inhibitory proteins identified in Streptomyces spp. A null mutant of the gene encoding BLIP-II (bliB::$hyg^r$) showed a bald appearance on solid media. Although BLIP-II was initially isolated from the supernatant of submerged cultures, sites of BLIP-II accumulation were seen in the cell envelope. Mutation of bliB was also associated with changes in the formation of septa and condensation of the chromosomal DNA associated with sporulation. The bliB mutant exhibited infrequent septa, showing dispersed chromosomal DNA throughout the mycelium, whereas the condensed chromosomes of the wild-type were separated by regularly spaced septa giving the appearance of a string of beads. Therefore, on the basis of these results, it is suggested that BLIP-II is a regulator of morphological differentiation in S. exfoliatus SMF19.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.4
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• pp.76-82
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• 2006

Flutter analyses are performed for the KF-16D aircraft with brand new ALQ-X ECM pod. A flutter analysis method using test modal data is proposed and validated using published F-16 modal data and flutter analysis results. Ground vibration test is performed for KF-16D stands on its landing gears. Attained modal data are transformed to free-free condition of KF-16D aircraft with ALQ-X pod and ALQ-119 pod, respectively. As the results of comparison of flutter analyses, ALQ-X is cleared to be operated in the flight envelope authorized for existing ECM pods.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.18
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• pp.65-104
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• 2002

With the current evolution from paper to electronic records the documentary credit market has been looking to ICC to provide guidance in this transition. To allow bankers and traders to familiarize themselves with this trend, eUCP has come into force from April 1st 2002. The eUCP provides definitions to allow current UCP terminology to accommodate the presentation of the equivalent of paper documents electronically and to provide necessary rules to allow the UCP and eUCP to work together. It is important to understand that many of the Articles of the UCP are not impacted by the presentation of electronic equivalent of paper documents and do not require any changes to accommodate electronic presentation. When read together the UCP and the eUCP will be broad enough to allow for developing practice in this area. The eUCP is not a revision of the UCP. The UCP will continue to provide the industry with rules for paper letters of credit in conjunction with the eUCP. This study comments that some characteristics of the eUCP have unique properties, such as juncture, envelope, homotheticity functions, and some applicable interpretations including abstraction of letter of credit, notice of completeness, notice of discrepancies and corruption of electronic records.

• The Journal of Korean Physical Therapy
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• v.10 no.2
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• pp.113-125
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• 1998

The neurotropic psudorabies virus(PRV) to replicate within neurons is very useful pathogen for neuronal tracing. I carried out this study to investigate the parametric analysis on the viral infection in the rat circadian control center with two genetically engineered strains out of PRV. The two strains are isogenic with the attenuated Bartha strain of PRV ; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu ; $4.75\times10^8pfu/ml$) and the other strain contained a PRV envelope glycoprotein gene(PRV-D ; $2.5\times10^8pfu/ml$) theat is absent in PRV-BaBlu. simultaneous or temporally separated sequential injection of$2{\mu}l$ of each strain into the vetreous body of eye produced a course of transsynaptic infection of retinohypothalamic circuitry. The results were as follows; 1. PRV-BaBlu and PRV-D infected the suprachiasmatic nucleus in hypothalamus and intergeniculate leaflet in lateral geniculate nucleus of thalamus. 2. The rate of PRV infection was dependent upon PRV strain. 3. Pre-infected neurons by PRV-D were interfered with the replication of PRV-BaBlu. 4. Dual injection of PRV-D and PRV-BaBlu showed more virulent than the parental strain.

• Journal of environmental and Sanitary engineering
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• v.13 no.1
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• pp.82-90
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• 1998

The study was carried out to investigate on the response of environmental sanitation problems in action of the volume based waste charge system in Seoul. The responses from the 396 persons who dwelled in Seoul was stimulatively answered, this system was considered as a successful one 69.6% of total answers, but little portion (21.2%) of those was unknown on the mean of this system. And we knew that this system was need to the communication and education through the press media about this one. After the action of the volume based waste charge system, the volume of refuse discharged in a day was decreased 30-50% than before. In the problems of environmental sanitation after this system, the number of sanitary insect(cockroach etc) and rat was decreased, also the production of order and dust caused by refuse was decreased in comparison with those before. This results were considered that the Separate Collection should be perfectly performed. In response, the regular envelope of refuge was serious problems, the improvement for this one was demanded, The kind of difficult refuse treated was waste foodstuffs> electric products> rubbers> furniture> bottles, cans>papers, pulps etc. and the next proposal for this system should be prepared. Conclusively, the volume based waste charge system should be remarkable system in terms of resources recycling as well as wastes reduction. Above all in order to conform this system the separate collection system should be systematically performed, the next improvable proposal of the detailed methods in this system should be prepared.

• The Plant Pathology Journal
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• v.31 no.1
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• pp.1-11
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• 2015

Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. Antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. Most antimicrobial cyclic peptides affect the integrity of the cell envelope. This is achieved through direct interaction with the cell membrane or disturbance of the cell wall and membrane component biosynthesis such as chitin, glucan, and sphingolipid. These are specific and selective targets providing reliable activity and safety for non-target organisms. Synthetic cyclic peptides produced through combinatorial chemistry offer an alternative approach to develop antimicrobials for agricultural uses. Those synthesized so far have been studied for antibacterial activity, however, the recent advancements in powerful technologies now promise to provide novel antimicrobial cyclic peptides that are yet to be discovered from natural resources.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.147-153
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• 2011

The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.4 s.109
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• pp.404-413
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• 2006

Induction motors are the workhorse of our industry because of their versatility and robustness. The diagnosis of mechanical load and power transmission system failures is usually carried out through mechanical signals such as vibration signatures, acoustic emissions, motor speed envelope. The motor faults including mechanical rotor imbalances, broken rotor bar, bearing failure and eccentricities problems are reflected in electric, electromagnetic and mechanical quantities. The recent research has been directed toward electrical monitoring of the motor with emphasis on inspecting the stator current of the motor, The stator current spectrum has been widely used for fault detection in induction motor systems. The motor current signature analysis is the useful technique to assess machine electrical condition. This paper describes the motor condition detected by the current signatures Paralleled with vibration signatures analysis of induction motors with the roller bearing and the journal bearing type for large vertical pumps in power plant as examples to discuss for motor fault detection and diagnosis.

• The Korean Journal of Mycology
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• v.16 no.3
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• pp.157-161
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• 1988

The vegetative nuclear divisions in hyphae and chromosome numbers were studied with the aid of Giemsa-HCl techniques from 10 strains of Fusarium oxysporum. The entire nuclear division process occurred within an intact nuclear envelope like other fungus. The results confirmed that 2 strains(F. oxysporum S Hongchun D2, F. oxysporum S Jinyang 4) were n=4; 3 strains(F. oxysporum f. sp. lini KFCC 32585, F. oxysporum f. sp. melongenae KFCC 34743 and F. oxysporum f. sp. raphani) n=5; 2 strains(F. oxysporum f. sp. vasinfectum, and F. oxysporum f. sp. mori KFCC 34742) n=6; 3 strains(F. oxysporum f. sp. cucumerium, F. oxysporum f. sp.niveum, and F. oxysporum f. sp. pisi) n=7.