• Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.125-129
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• 2013

Vibrio ichthyoenteri is a facultatively anaerobic gram-negative bacterium with straight or slightly curved rod morphology. The bacterium is an etiological agent of bacterial enteritis of Olive flounder Paralichthys olivaceus. Only a handful of studies, using a limited number of isolates, have investigated the phenotypic and genetic characteristics of V. ichthyoenteri. We isolated 40 V. ichthyoenteri strains, identified based on the 16S rRNA gene sequence, from the diseased flounder larvae and investigated the API 20E and ZYM profiles. The isolates exhibited highly divergent phenotypic characteristics regardless of sampling time point and location, and fish age. Essential enzymes produced by V. ichthyoenteri seemed to be alkaline phosphatase, leucine arylamidase, and N-acetyl-${\beta}$-glucosaminidase. This study reveals a much greater enzymatic and biochemical phenotype diversity than has been evident to date. These results suggest that a given population of V. ichthyoenteri could be heterogeneous in terms of its phenotypic characteristics.

• Korean Journal of Pharmacognosy
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• v.17 no.3
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• pp.215-222
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• 1986

Although the 'Insamyangwee-tang' has been widely used in clinical purposes in the oriental medicine, its clinical efficacy is only documentated for the cases of gastritis, gastric ulcer and enteritis, but the experimental study on these has not been undertaken. So, to investigate the clinical efficacy of 'Insamyangwee-tang' comparing with animal experiments, This study was carried out. The results showed that relaxing action was shown on the isolated ileum in mice and that strong antagonistic actions were seen on $BaCl_2$, acetylcholine and histamine induced contraction of the ileum in mice, rats, rabbits and guinea-pigs that the relaxing effect of the intestinal smooth muscle was recognized. Inhibitory effects on transport rate in the small intestine of mice. Strong antagonistic actions were seen on acetylcholine induced contraction of duodenum in rats and remarkably inhibiting actions were seen of duodenum in rats. Inhibitory action on the secretion of gastric juice and pepsin, anti-ulceration effect was recognized.

• Journal of Chest Surgery
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• v.30 no.1
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• pp.97-102
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• 1997

We have experienced two cases of mycotic aneurysms of the superior mesenteric artery The first case originated from septic embolism of Infective endocarditis and the second case originated from salmonella enteritis eight months before The aneurysms were diagnosed by abdominal ultrasonography and comflrmed by computed tomogram and dngiography but the blood culture was negative in both cases at the time of the surgery. Both patient ere successfully treated by resection only and the restorations of vdsculdr continuity were not neccesary because of adequate collateral circulations to the intestine. Both pd.tient's postoperative courses were uneventful after the follow up of one year and nine months, respectively.

• Korean Journal of Veterinary Research
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• v.50 no.1
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• pp.59-62
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• 2010

A 2-year-old lineolated parakeet (Bolborhynchus lineola) was presented with abdominal distention and respiratory distress for two months. The bird was poorly fleshed and the liver was enlarged on coelomic palpation. Plain and contrast radiographic examinations exhibited hepatomegaly and distended intestinal loop, which compromised the air sacs. Multifocal hyperechogenecity was observed in the liver on ultrasonography. Postmortem gross examination revealed hepatomegaly with numerous pinpoint tan foci in the hepatic parenchyma and distended small intestine filled with adult ascarids. Microscopically, granulomatous hepatitis and enteritis infected by intrahistiocytic acid-fast bacilli were evident. Polymerase chain reaction indicated that the acid-fast bacilli were Mycobacterium avium subsp. avium.

• Journal of fish pathology
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• v.21 no.1
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• pp.21-27
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• 2008

Mass mortality occurred in mud loaches, Misgurnus mizolepis, cultured in ponds located in Kunsan. External signs of affected fish showed hemorrhage of skin and fins, Internally, pale liver with congestion, enlarged kidney, and spleen and enteritis exhibited. Causative bacteria isolated from liver, spleen, and kidney of the disease fish. In biochemical tests, the isolates were similar with those of the reference strains, A. sobria. The aerolysine gene from the present isolate was amplified PCR with the primer SOBF and SOBB for A. sobria. The isolate was identified as A. sobria on the basis of those tests. In virulence test, the present isolate resulted in the development of clinical signs identical to those in naturally infected fish. The present results conclude that the present isolate is A. sobria and can be a pathogen which causes motile aeromonad septicemia to mud loach.

• Biomolecules & Therapeutics
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• v.16 no.4
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• pp.403-409
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• 2008

Eclipta prostrata grows abundantly in the tropical and the sub-tropical parts of the world including most part of the Korean Peninsula. The plant has been traditionally used for the treatment of a number of inflammatory diseases including hepatitis and enteritis but the nature of its immuno-modulating activity needs more studies. In this study, water-soluble sugar-containing fractions were purified from the herb and their effects on the culture of mouse splenocytes were examined. One of the fractions significantly suppressed apoptosis of the splenocytes in culture, which involves the gene expression regulation of a number of cytokines and cytokine receptors including MIP1-$\beta$. This study could explain an immunological activity of Eclipta prostrata and would lead to identify an immuno-active compound from the plant.

• Korean Journal of Veterinary Service
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• v.26 no.2
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• pp.185-191
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• 2003

There was a mass outbreak of death caused by transport stress and malnutrition in Japanese macaques imported from Japan at a farm stock in Korea, which were diagnosed on the ground of history taking, clinical finding, hematological examination and necropsy. Twenty eight Japanese macaques imported from Japan died suddenly from the 4th day after arrival in a farm. These animals were confined very small cages individually and lose the appetite for 10 days from shipping. Main clinical findings observed from alive Japanese macaques consisted of hypothermia and dehydration. On hematological observation for typical 4 cases showed increased erythrocytes and packed cell volume. On serum biochemical examination for 3 cases showed hypoglycemia, whereas blood urea nitrogen, aspartate aminotransferase and alanine aminotransferase were increased. On necropsy for 3 cases, hemorrhage and congestion were observed on the lung and the walls of stomach and intestine were thinned. Histologically, hemorrhagic pneumonia and enteritis with loss of villi were seen. The death of these animal was caused by poor management during transport, which was resulted in transport stress and malnutrition.

• Korean Journal of Veterinary Service
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• v.41 no.2
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• pp.105-110
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• 2018

A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.

• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.171-174
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• 1982

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Korea since June, 1931 and it was followed serologically and electron microscopically for the clarification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in immuno-diffusion. Electron micrograph of the material revealed parvovirus particles with size of 20nm and icosahedral structure. These results clearly indicated that CPV was the primary cause of canine epidemic prevailing in 1981 in Korea.

• Plant Resources
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• v.6 no.2
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• pp.108-113
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• 2003

This study was carried out to obtain basic information for possibility of oral vaccine in carrot using Agrobacteruim -mediated transformation system. The epitope region of porcine epidemic diarrhea virus (PEDV) spike gene which is classified as a member of the Coronaviridae and causes an acute enteritis in pigs was successfully expressed in carrot (Daucus carota) using the Agrobacterium-mediated transformation system. Hypocotyl segments of in vitro germinated plantlets were infected with Agrobacteriun tumefaciens LBA 4404 harboring PEDV spike gene. Embryogenic callus (EC) was induced on MS selection medium with 1 mg/L 2,4-D, 50 mg/L kanamycin and 300 mg/L cefotaxime after 45 days of culture. Subcultured ECs on MS selection medium without 2,4-D were converted to somatic embryos (SE) of various stage; globular, heart and torpedo stage. Putative transgenic embryos were selected on MS medium with 50 mg/L kanamycin and 300 mg/L cefotaxime. Regenerated plantlets from transformed SE were induced on MS medium containing 50 mg/L kanamycin after 30 days of culture. Genomic PCR confirmed the integration of PEDV spike gene into nuclear genome of carrot and northern blot analysis demonstrated the expression of PEDV spike gene in transgenic carrot.