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Isolation of Aeromonas sobria from Cultured Mud Loach, Misgurnus mizolepis  

Yu, Jin-Ha (Department of Aquatic life Medicine, Kunsan National University)
Park, Sung-Woo (Department of Aquatic life Medicine, Kunsan National University)
Publication Information
Journal of fish pathology / v.21, no.1, 2008 , pp. 21-27 More about this Journal
Abstract
Mass mortality occurred in mud loaches, Misgurnus mizolepis, cultured in ponds located in Kunsan. External signs of affected fish showed hemorrhage of skin and fins, Internally, pale liver with congestion, enlarged kidney, and spleen and enteritis exhibited. Causative bacteria isolated from liver, spleen, and kidney of the disease fish. In biochemical tests, the isolates were similar with those of the reference strains, A. sobria. The aerolysine gene from the present isolate was amplified PCR with the primer SOBF and SOBB for A. sobria. The isolate was identified as A. sobria on the basis of those tests. In virulence test, the present isolate resulted in the development of clinical signs identical to those in naturally infected fish. The present results conclude that the present isolate is A. sobria and can be a pathogen which causes motile aeromonad septicemia to mud loach.
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