[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7853/kjvs.2018.41.2.105

Identification of Salmonella spp. from porcine salmonellosis by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Yang, Hyoung-Seok (Jeju Self-Governing Provincial Veterinary Research Institute)
Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)

Publication Information

Korean Journal of Veterinary Service / v.41, no.2, 2018 , pp. 105-110 More about this Journal

Abstract

A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.

Keywords

Porcine; Salmonellosis; Identification; PCR; MALDI-TOF MS;

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Times Cited By KSCI : 1 (Citation Analysis)

Reference
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