• Preventive Nutrition and Food Science
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• v.14 no.2
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• pp.168-171
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• 2009

The volatile aroma constituents of Eclipta prostrata L. (leaves, stems, and flowers) were isolated by hydro-distillation extraction method and analyzed by GC/MS. The yield of Eclipta prostrata L. essential oil was 0.1% (v/w), and its color was yellow. Sixty-eight volatile flavor compounds, which make up 71.15% of the total volatile composition of the essential oil were tentatively characterized. It contained 35 hydrocarbons (56.25%) with sesquiterpene predominating, 12 alcohols (3.05%), 8 ketones (3.83%), 9 aldehydes (1.86%), 2 oxides (6.03%), and 2 esters (0.13%). ${\alpha}$-Humulene, 6,9-heptadecadiene, (E)-${\beta}$-farnesene, and ${\alpha}$-phellandrene were the major abundant aroma components in Eclipta prostrata L., aromatic and medicinal plant.

• The Journal of Korean Medicine
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• v.39 no.1
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• pp.63-74
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• 2018

Objectives: Periodontitis is an inflammatory disease with the destruction of periodontal ligament, alveolar bone loss and inflammation of gingva, leading to teeth loss. Eclipta prostrata L. (Ecliptae Herba) has been used to treat the inflammatory disease as a Korean traditional medicine. The aim of this study is to investigate the effects of E. prostrata L. on periodontitis. Methods: E. prostrata L. was extracted with water and lyophilized. The aqueous extract of E. prostrata L. (EP) was topically applied to the periodontal lesion for 2 weeks. To induce the periodontitis, a 3-0 nylon ligature was placed around the cervix of the lower first molar in rat. Rats were divided into 3 groups (n = 7); NL group (non-ligatured and non-treated), L group (ligatured and vehicle-treated) and EP group (ligatured and EP-treated). After sacrifice, the mandibles was dissected and stained with methylene blue solution to analyze the alveolar bone loss. The expression of MMP-9 was determined in gingival tissues. To confirm the effect of EP on recovery of gingiva, mRNA expressions of type I pro-collagen and MMP-9 levels were investigated in LPS-treated HS68 fibroblast cells. In addition, inflammatory mediators were evaluated in LPS-treated RAW264.7 cells. Results: Alveolar bone loss was significantly inhibited by EP treatment. The mRNA expression of MMP-9 was attenuated in rats treated with EP. In addition, treatment with EP increased the expression of type I pro-collagen, while the expression of MMP-9 was decreased in LPS-stimulated HS68 fibroblast cells. Furthermore, EP down-regulated the LPS-induced IL-6, $TNF-{\alpha}$, COX-2 and iNOS production in RAW264.7 cells. Conclusions: These results suggest that EP have ameliorative effects on periodontitis through inhibiting alveolar bone loss and modulating the inflammatory mediators. Therefore, E. prostrata L. may be an alternative on patients with periodontitis.

• Natural Product Sciences
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• v.19 no.3
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• pp.231-235
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• 2013

Activity-guided isolation to search for antifibrotic compounds from natural products using HSC-T6 cells afforded nine flavonoids or phenolics, luteolin (1), 3'-O-methylorobol (2), acactin 7-rutinoside (3), sedelolactone (4), 4-methoxyphenol (5), 4-hydroxyaldehyde (6), 4-hydoxyaldehyde (7), 4-hydroxy-3-methoxybenzoic acid (8), and ferulic acid (9) from the methanolic extract of aerial parts of Eclipta prostrata L.. Among the isolated compounds, luteolin (1) significantly inhibited the proliferation of HSCs in dose- and time-dependent manners. Antifibrotic activity of E. prostrata and its phenolic compounds might provide potential therapeutical choice in the treatment of hepatic fibrosis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.30 no.6
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• pp.432-438
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• 2016

This study was peformed to investigate the mechanism of ethanol extract of Eclipta Prostrata (L.) L. (EEP) induced melanogenesis. EEP enhanced tyrosinase activity and melanin contents of B16F10 cells. Moreover, EEP increased the protein expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). But EEP did not increase the protein expression of tyrosinase-related protein 2 (TRP-2). These results suggest that melanogenesis-promoting effect of EEP was involved in regulation of tyrosinaase and TRP-1 protein, and EEP may be a potent pigmentation darkening agent in hypopigmentation condition.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.299-307
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• 1988

Several experiments were conducted to investigate the effects of external factors on germination and emergence of Eclipta prostrata (L.)L. The weight of viable achenes doubled as a result of 90 minutes soaking in water. The germination of E. prostrata was significantly improved by alternating temperatures. At a constant temperature of $35^{\circ}C$, only 78% of the achenes germination, whereas at alternating temperatures of 35/$20^{\circ}C$, 96.5% of the achenes germinated. E. prostrata was more sensitive than rice to moisture stress. No germination of E. prostrata achenes occurred in the absence of oxygen. No germination of E. prostrata achenes occurred in the dark or when they were exposed to green, blue, and far-red light. Germination of E. prostrata achenes was influenced by the duration of illumination after absorption of water. Ten hours of illumination was needed for maximum germination and 2 hours for 50% germination. No significant changes in germination of E. prostrata achenes were observed between pH 3 and 10. A high tolerance of E. prostrata achenes to salt was observed. Emergence of E. prostrata achenes was greatly affected by planting depth. In the upland soil, 74.0% of the achenes planted on the soil surface germinated, and no emergence was at planting depths of 0.5 cm or greater.

• Natural Product Sciences
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• v.26 no.4
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• pp.326-333
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• 2020

The aerial parts of Eclipta prostrata is used as a traditional medicine and vegetable. In traditional folk medicine, it is used for treatment of hemorrhages, hepatic, disease, renal injuries, hair loss, tooth mobility, and viper bites. In this study, ten compounds (1 - 10) were isolated from the aerial parts of E. prostrata. A reliable high performance liquid chromatography equipped with photometric diode array detector (HPLC-PDA) method was developed to simultaneously quantitate 10 marker compounds [chlorogenic acid (1), paratensein 7-O-��-ᴅ-glucoside (2), quercetin 7-O-��-ᴅ-glucoside (3), luteolin 7-O-��-ᴅ-glucoside (4), apigenin 7-O-��-ᴅ-glucoside (5), apigenin 4'-O-��-ᴅ-glucoside (6), apigenin (7), luteolin (8), wedelolactone (9), and paratensein (10)]. In addition, compounds 5 and 6 showed considerable inhibitory effects against protein-tyrosine phosphatase 1B (PTP1B) enzyme. Moreover, compounds 6 - 8, and 10 exhibited potent α-glucosidase inhibitory effects with IC50 values of 24.5 ± 1.9, 33.0 ± 0.5, 45.5 ± 0.1, and 23.8 ± 1.0 µM, respectively. All compounds (1 - 10) showed considerable acetylcholinesterase (AChE) inhibitory effects with IC50 ranging from 30.1 to 75.2 µM.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.3
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• pp.349-362
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• 2018

An annual plant, Eclipta prostrata (Linn) is a member of the Asteraceae plant family and inhabited in tropical or subtropical regions of the world. Through many previous researches, E. prostrata has been extensively studied for its hepatoprotective effect, antivenom potential against viper venom, antioxidant, hair-growth, wound-healing efficacy and so on. In this study, for better understanding of the potential of E. prostrata as skin protectant, we conducted the experiments evaluating the antioxidant and antiaging efficacy. To this end, 50% ethanolic extract of E. prostrata and its ethyl acetate fraction were prepared and investigated. For the evaluation of antioxidant capacity of the samples, $FSC_{50}$ and $OSC_{50}$ were estimated. As a result, $OSC_{50}$ of ethyl acetate fraction was 2.7 times superior to $OSC_{50}$ of L-ascorbic acid, a well known antioxidant agent. Futhermore E. prostrata showed notable reactive oxygen species (ROS) scavenging effect and protective effect against $H_2O_2$ in the celluar level as well. Especially, in the $^1O_2$ induced hemolysis test, $64{\mu}g/mL$ of ethyl acetate fraction showed greater than 6 times increased retardation effect compare to control which means E. prostrata has remarkable antioxidant capacity. To validate the antiaging effect of the samples, we conducted elastase inhibition assay using elastase solution extracted from human skin fibroblasts, Hs68. As a result, $16{\mu}g/mL$ of each sample showed 6.8% and 14.0% of elastase inhibition respectively. Finally, antimicrobial activity of E. prostrata was assessed to validate the possibility as alternative preservative. From the result, ethyl acetate fraction showed oustanding antimicrobial activity as of methyl paraben, a well known chemical preservative. In conclusion, these results suggest that E. prostrata can be used as natural skin protectant or preservative as natural ingredient in food or cosmetics industry.

• Journal of Fashion Business
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• v.21 no.1
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• pp.166-176
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• 2017

This study is fundamental research on how to be able to scientifically quantify and reproduce a natural dyeing procedure. By dyeing silk fabrics, the establishment of a reproducible dyeing method was sought. Juglans mandshurica has been known as one of the most widely used black vegetable dyes. Repetition conditions and combination dyeing were performed with Juglans mandshurica cortex, gallnut, clove, and Eclipta prostrata L. extracts to express a deep black color. Juglans mandshurica cortex, gallnut, clove, and Eclipta prostrata L. extracts were suitable for black coloration and showed a darker black color when combined with iron mordant. Specifically, Juglans mandshurica cortex and clove can be used for deep black coloring. Color fastness when washed or dry cleaned was found to be strong with a grade of 4-5 and fastness to light was rated at a 3-4. The grade of color change when exposed to rubbing and perspiration was good at a 4-5. In regards to functional property aspects, it showed excellent results with a 99% deodorization rate at 120 minutes of dyeing time, 97.1% UV protection rate, and 85.9% and 62.3% bacterial reduction against Staphylococcus aureus and Streptococcus pneumoniae, respectively. Therefore, it is considered that the extract from the Juglans mandshurica cortex is of great value as an eco-friendly, natural dyestuff.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.281-282
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• 2012

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.309-316
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• 1988

Several experiments were conducted to investigate the achene viability and growth characteristics of Eclipta prostrata (L.) L. No dormancy and no after-ripening requirement were found for E. prostrata achenes. When achenes were stored at room temperature, germination did not decrease with up to 5 months storage. Large differences in loss of viability of E. prostrata achenes occurred when different dehydration methods were used. Immediate dehydration resulted in high viability, but slow dehydration resulted in severe loss of viability. Achene viability at shallow burial depths (5 and 10 cm deep) was lower under upland soil conditions than under lowland soil conditions. Seedling growth was greatly reduced when flooding to a depth of 10 cm occurred at or before the 4-leaf stage. Flooding after the 4-leaf stage stimulated stem elongation. Branching started from the second week and usually terminated at the tenth week. Leaf size was determined by the branch which are related to the assimilate supply. Flowering of E. prostrata started during the fifth week after emergence, and mature achenes were produced from the sixth week. Ten to 14 days were needed for the achenes to mature. About 14,000 achenes were produced on each plant. Achene production per week increased from the sixth week to the tenth week and thereafter it declined. The average number of achenes per inflorescence decreased with delay in flowering.