Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Development and Validation of an HPLC-PDA Method for Quantitation of Ten Marker Compounds from Eclipta prostrata (L.) and Evaluation of Their Protein Tyrosine Phosphatase 1B, α-Glucosidase, and Acetylcholinesterase Inhibitory Activities

  • Nguyen, Duc Hung (College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu) ;
  • Le, Duc Dat (College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu) ;
  • Ma, Eun Sook (College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu) ;
  • Min, Byung Sun (College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu) ;
  • Woo, Mi Hee (College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu)
  • Received : 2020.09.03
  • Accepted : 2020.12.16
  • Published : 2020.12.31
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Abstract

The aerial parts of Eclipta prostrata is used as a traditional medicine and vegetable. In traditional folk medicine, it is used for treatment of hemorrhages, hepatic, disease, renal injuries, hair loss, tooth mobility, and viper bites. In this study, ten compounds (1 - 10) were isolated from the aerial parts of E. prostrata. A reliable high performance liquid chromatography equipped with photometric diode array detector (HPLC-PDA) method was developed to simultaneously quantitate 10 marker compounds [chlorogenic acid (1), paratensein 7-O-��-ᴅ-glucoside (2), quercetin 7-O-��-ᴅ-glucoside (3), luteolin 7-O-��-ᴅ-glucoside (4), apigenin 7-O-��-ᴅ-glucoside (5), apigenin 4'-O-��-ᴅ-glucoside (6), apigenin (7), luteolin (8), wedelolactone (9), and paratensein (10)]. In addition, compounds 5 and 6 showed considerable inhibitory effects against protein-tyrosine phosphatase 1B (PTP1B) enzyme. Moreover, compounds 6 - 8, and 10 exhibited potent α-glucosidase inhibitory effects with IC50 values of 24.5 ± 1.9, 33.0 ± 0.5, 45.5 ± 0.1, and 23.8 ± 1.0 µM, respectively. All compounds (1 - 10) showed considerable acetylcholinesterase (AChE) inhibitory effects with IC50 ranging from 30.1 to 75.2 µM.

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References

  1. Kim, H. Y.; Kim, H. M.; Ryu, B.; Lee, J. S.; Choi, J. H.; Jang, D. S. Arch. Pharm. Res. 2015, 38, 1963-1969. https://doi.org/10.1007/s12272-015-0599-2
  2. Xi, F. M.; Li, C. T.; Han, J.; Yu, S. S.; Wu, Z. J.; Chen, W. S. Bioorg. Med. Chem. 2014, 22, 6515-6522. https://doi.org/10.1016/j.bmc.2014.06.051
  3. Fang, X.; Wang, J.; Hao, J.; Li, X.; Guo, N. Food Chem. 2015, 188, 527-536. https://doi.org/10.1016/j.foodchem.2015.05.037
  4. Han, L.; Liu, E.; Kojo, A.; Zhao, J.; Li, W.; Zhang, Y.; Wang, T.; Gao, X. Scientific World Journal 2015, 2015, 980890.
  5. Anhut, S.; Dietmar Zinsmeister, H.; Mues, R.; Barz, W.; Mackenbrock, K.; Köster, J.; Markham, K. R. Phytochemistry 1984, 23, 1073-1075. https://doi.org/10.1016/S0031-9422(00)82611-4
  6. Roberts, D. W.; Doerge, D. R.; Churchwell, M. I.; Gamboa da Costa, G.; Marques, M. M.; Tolleson, W. H. J. Agric. Food Chem. 2004, 52, 6623-6632. https://doi.org/10.1021/jf049418x
  7. Clifford, M. N.; Johnston, K. L.; Knight, S.; Kuhnert, N. J. Agric. Food Chem. 2003, 51, 2900-2911. https://doi.org/10.1021/jf026187q
  8. Nguyen, D. H.; Zhao, B. T.; Le, D. D.; Kim, Y. H.; Yoon, Y. H.; Ko, J. Y.; Woo, K. S.; Woo, M. H. Nat. Prod. Sci. 2016, 22, 140-145. https://doi.org/10.20307/nps.2016.22.2.140
  9. Nguyen, D. H.; Seo, U. M.; Zhao, B. T.; Le, D. D.; Seong, S. H.; Choi, J. S.; Min, B. S.; Woo, M. H. Bioorg. Chem. 2017, 72, 293-300. https://doi.org/10.1016/j.bioorg.2017.04.017
  10. Han, X. H.; Hong, S. S.; Hwang, J. S.; Lee, M. K.; Hwang, B. Y.; Ro, J. S. Arch. Pharm. Res. 2007, 30, 13-17. https://doi.org/10.1007/BF02977772
  11. Liu, Q. M.; Zhao, H. Y.; Zhong, X. K.; Jiang, J. G. Food Chem. Toxicol. 2012, 50, 4016-4022. https://doi.org/10.1016/j.fct.2012.08.007