Browse > Article

Antiproliferative Phenolics from Eclipta prostrata in the Activated Hepatic Stellate Cells  

Jeong, Eun Ju (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science and Technology)
Lee, Mi Kyeong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Young Choong (College of Pharmacy, Chungbuk National University)
Sung, Sang Hyun (College of Pharmacy, Chungbuk National University)
Publication Information
Natural Product Sciences / v.19, no.3, 2013 , pp. 231-235 More about this Journal
Abstract
Activity-guided isolation to search for antifibrotic compounds from natural products using HSC-T6 cells afforded nine flavonoids or phenolics, luteolin (1), 3'-O-methylorobol (2), acactin 7-rutinoside (3), sedelolactone (4), 4-methoxyphenol (5), 4-hydroxyaldehyde (6), 4-hydoxyaldehyde (7), 4-hydroxy-3-methoxybenzoic acid (8), and ferulic acid (9) from the methanolic extract of aerial parts of Eclipta prostrata L.. Among the isolated compounds, luteolin (1) significantly inhibited the proliferation of HSCs in dose- and time-dependent manners. Antifibrotic activity of E. prostrata and its phenolic compounds might provide potential therapeutical choice in the treatment of hepatic fibrosis.
Keywords
Eclipta prostrata; Antifibrotic activity; HSC-T6; Hepatic stellate cells;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Li, C.C., Xie, Z.X., Zhang, Y.D., Chen, J.H., and Yang, Z., Total Synthesis of Wedelolactone. J. Org. Chem. 68, 8500-8504 (2003).   DOI
2 Ma-Ma, K., Nyunt, N., and Tin, K.M., The protective effect of Eclipta alba on carbon tetrachloride-induced acute liver damage. Toxicol. Appl. Pharmacol. 45, 723-728 (1978).   DOI
3 Li, D. and Friedman, S.L., Liver fibrogenesis and the role of hepatic stellate cells: new insights and prospects for therapy. J. Gastroenterol. Hepatol. 14, 618-633 (1999).   DOI
4 Li, J., Kadota, S., Kawata, Y., Hattori, M., Xu, G., Namba, Tsuneo., Constituents of the roots of Cynanchum bungei Decne. Isolation and structures of four new glucosides, bungeiside A, B, C, and D. Chem. Pharm. Bull. 40, 3133-3137 (1992).   DOI
5 Lin, Y.L., Lee, T.F., Huang, Y.J., and Huang, Y.T., Antiproliferative effect of salvianolic acid A on rat hepatic stellate cells. J. Pharm. Pharmacol. 58, 933-939 (2006).   DOI
6 Melo, P.A., Nascimento, M.C., and Mors, W.B., Suarez-Kurtz, G., Inhibition of the myotoxic and hemorrhagic activities of crotalid venoms by Eclipta prostrata (Asteraceae). Toxicon. 32, 595-603 (1994).   DOI
7 Owen, R.W., Haubner, R., Mier, W., Giacosa, A., Hull, W.E., Spiegelhalder B., and Bartsch H., Isolation, structure elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes. Food Chem. Toxicol. 41, 703-717 (2003).   DOI
8 Park, J.C., Lee, J.H., and Choi, J.S., A flavone diglycoside from Cirsium japonicum var. ussuriense. Phytochem. 39, 261-262 (1995).   DOI
9 Sakata, R., Ueno, T., Nakamura, T., Sakamoto, M., Torimura, T., and Sata, M., Green tea polyphenol epigallocatechin-3-gallate inhibits platelet-derived growth factor-induced proliferation of human hepatic stellate cell line LI90. J. Hepatol. 40, 52-59 (2004).
10 Saxena, A.K., Singh, B., and Anand, K.K., Hepatoprotective effects of Eclipta alba on subcellular levels in rats. J. Ethnopharmacol. 40, 155-161 (1993).   DOI
11 Vogel, S., Piantedosi, R., Frank, J., Lalazar, A., Rockey, D.C., Friedman, S.L., and Blaner, W.S., An immortalized rat liver stellate cell line (HSC-T6): a new cell model for the study of retinoid metabolism in vitro. J Lipid Res. 41, 882-893 (2000).
12 Stalin, T. and Rajendiran, N., A study on the spectroscopy and photophysics of 4-hydroxy-3-methoxybenzoic acid in different solvents, pH and $\beta$-cyclodextrin. Journal of Molecular 794, 35-45 (2006).
13 Tsukada, S., Parsons, C.J., and Rippe, R.A., Mechanisms of liver fibrosis. Clin. Chim. Acta 364, 33-60 (2006).   DOI
14 Uyama, N., Shimahara, Y., Okuyama, H., Kawada, N., Kamo, S., Ikeda, K., and Yamaoka, Y., Carbenoxolone inhibits DNA systhesis and collagen gene expression in rat hepatic stellate cells in culture. J. Hepatol. 39, 745-799 (2003).
15 Wagner, H., Geyer, B., Kiso, Y., Hikino, H., and Rao, G.S., Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea. Planta Med. 5, 370-374 (1986).
16 Wang, W., Zhao, Y.Y., Liang, H., Jia, Q., and Chen, H.B., Coumestans from Hedysarum multijugum. J. Nat. Prod. 69, 876-880 (2006).   DOI
17 Wu, J. and Zern, M.A., Hepatic stellate cells: a target for the treatment of liver fibrosis. J. Gastroenterol. 35, 665-672 (2000).   DOI
18 Zhao, W., Liang, C., Chen, Z., Pang, R., Zhao, B., and Chen, Z., Luteolin inhibits proliferation and collagen synthesis of hepatic stellate cells. Zhonghua Gan Zang Bing Za Zhi. 10, 204-206 (2002).
19 Anselmi, C., Centini, M., Ricci, M., Buonocore, A., Granata, P., Tsuno, T., and Facino, R.M., Analytical characterization of a ferulic acid /- cyclodextrin inclusion complex. Journal of Pharm. Biomed. Anal. 40, 875-881 (2006).   DOI
20 Bae, K.H., Dictionary of Korean Folk Medicine. Kyu Hak Sa, Seoul p. 297 (2000).
21 Bataller, R. and Brenner, D.A., Liver fibrosis. J. Clin.Invest. 115, 209-218 (2005).   DOI
22 Dean, W.R., Daniel, R., and Doerge, M.I., Churchwell Goncalo Gamboa da Costa M., Matilde, M., William, H.T., Inhibition of Extrahepatic Human Cytochromes P450 1A1 and 1B1 by Metabolism of isoflavones Found in Trifolium pretense. J. Agri. Food Chem. 52, 6623-6632 (2004).   DOI
23 Chen, A. and Zhang, L., The antioxidant (-)-epigallocatechin-3-gallate inhibits rat hepatic stellate cell proliferation in vitro by blocking the tyrosine phosphorylation and reducing the gene expression of plateletderived growth factor-beta receptor. J. Biol. Chem. 278, 23381-23389 (2003).   DOI
24 Friedman, S.L., Liver fibrosis - from bench to beside. J. Hepatol. 38, S38- S53 (2003).
25 Chen, Y.W., Li, D.G., Wu, J.X., Chen, Y.W., and Lu, H.M., Tetrandrine inhibits activation of rat hepatic stellate cells stimulated by transforming growth factor-b in vitro via upregulation of Smad 7. J. Ethnopharmacol. 100, 299-305 (2005).   DOI
26 Emmanuel, S., Amalraj, T., and Ignacimuthu, S., Hepatoprotective effect of coumestans isolated from the leaves of Wedelia calendulacea less. in paracetamol-induced liver damage. Indian J. Exper. Biol. 39, 1305- 1307 (2001).
27 Geerts, A., History, heterogeneity, developmental biology, and functions of quiescent hepatic stellate cells. Semin Liver Dis. 21, 311-335 (2001).   DOI
28 Hosny, M. and Rosazza John P.N., Microbial hydroxylation and methylation of genistein by Streptomycetes. J. Nat. Prod. 62, 6909- 6912 (1999).
29 Knuutinen, J., Autio, P., Klein, P., Kivela, S., Virkki, L., and Lahtipera, M., Synthesis and structure verification of chlorinated 4- Methoxyphenols, models of metabolites of chlorophenolic compounds. Chemosphere 17, 1821-1829 (1988).   DOI
30 Kumari, C.S., Govindasamy, S., and Sukumar, E., Lipid lowering activity of Eclipta prostrata in experimental hyperlipidemia. J. Ethnopharmacol. 105, 332-335 (2006).   DOI
31 Lee, M.K., Ha, N.R., Yang, H., Sung, S.H., Kim, G.H., and Kim, Y.C., Antiproliferative activity of triterpenoids from Eclipta prostrata on hepatic stellate cells. Phytomedicine 15, 775-780 (2008).   DOI