• Journal of Pharmaceutical Investigation
• /
• v.40 no.3
• /
• pp.139-153
• /
• 2010

Nitrosative stress is defined as pathophysiological conditions that are related to covalent modifications of proteins by nitration/nitrosylation by forms of nitrogen oxide ($NO_x$), leading to DNA damage, ultimately, cell death. This type of stress condition appears to be associated with a number of disease states, including diabetes, inflammation and neurodegenerative diseases. Since these pathological conditions are frequently chronic in nature and, thus, require long-term treatment, changes in pharmacokinetics are likely to affect the therapy. Transporters are membrane proteins that facilitate the movement of substrates, including drugs, across plasma membranes of epithelial / endothelial cells. Since it is now increasingly evident that transporters are pharmacokinetically significant, functional alteration of transporters by this stress condition may have therapeutic relevance. In this review, experimental techniques that are used to study both in vivo and in vitro nitrosative stress are summarized and discussed, along with available literature information on the functional implication of transporters under conditions of nitrosative stress conditions. In the literature, both functional induction and impa irment were apparently present for both drug transporter families [i.e., ATP-binding cassette (ABC) and solute carrier families (SLC)]. Furthermore, a change in the function of a certain transporter appears to have temporal dependency by impairment in the early phase of nitrosative stress and induction thereafter, suggesting that the role of nitrosative stress is complex in terms of functional implications of the transporters. Although the underlying mechanisms for these alterations are not fully understood, protein nitration/nitrosylation appears to be involved in the functional impairment whereas transcript factor(s) activated by nitrosative stress may play a role, at least in part, in functional induction. Interestingly, functional induction under conditions of nitrosative stress has not been observed for SLC transporters while such impairment has been documented for both ABC and SLC transporters. Further investigations appear to be necessary to fully delineate the underlying reasons for these differences on the impact and importance of nitrosative stress conditions.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.2
• /
• pp.186-192
• /
• 2001

A primary culture of human fetal hepatocytes was obtained through a therapeutic abortion process at 26 weeks of gestation period. More than $10^8$ cells were seeded on a plastic plate. These hepatocytes were infected with hepatitis B virus (HBV). The HBV was purified from serum of one chronic HBV carrier. Transformed hepatocytes were subcultured in a 10% FBS-supplemented medium. The morphology of the transformed cell was epithelial-like. The cells from the first pass showed signs of early proliferation and had a latent period of more than 3 months after 6-7 passages. After the rest period, the transformed cell proliferated actively and they were subcultured every three days. Transformed hepatocytes were characterized by detection of the HBV transcript by RT-PCR. The secretion of virions from transformed cells was investigated by PCR with the cell medium. Two types of virions secreted into the culture medium were examined by using the transmission electron microscope. Another approach to study the secretion of virions in to culture medium was carried out with HBV antibody. HBsAg was detected in the culture medium of transformed cells using ELISA and Western blot analyses. These data suggested that the human fetal hepatocyte cell line has been established by infection of HBV, in which this cell line secreted viral particles into the culture medium.

• Korean Journal of Computational Design and Engineering
• /
• v.11 no.2
• /
• pp.115-127
• /
• 2006

At the initial design stage, the generation process of the production material information of a building block and the simulation process of the block erection, which are required to perform the production planning and scheduling, have been manually performed by using 2D drawings, data of parent ships, and design experiences. To make these processes automatic, the accurate generation method of the production material information and the convenient simulation method of the block erection using the 3D CAD model, which was generated from the initial hull structural design system early developed by us, were proposed in this study. For this, a 3D CAD model for a whole hull structure was generated first, and the block division method for dividing the 3D CAD model into several building blocks was proposed. The generation method of the production material information for calculating the weight, center of gravity, painting area, joint length, etc. of a building block was proposed as well. Moreover, the simulation method of the block erection was proposed. Finally, to evaluate the efficiency of the proposed methods for the generation of the production material information and the simulation of the block erection, these methods were applied to corresponding processes of a deadweight 300,000 ton VLCC (Very Large Crude oil Carrier). As a result, it was shown that the production material information of a building block can be accurately generated and the block erection can be conveniently simulated in the initial design stage.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.18 no.5
• /
• pp.439-445
• /
• 2012

Only the sand carriers fitted with hopper doors can remove their hatch covers in current regulations. However, there is no sand carriers fitted with hopper doors in korea. Through the analysis of accidents, inspection of equipment installed on ships, and evaluation of stability and seakeeping performance concerning the sand carriers, it is expected that the current exemption requirement is modified for hatch covers for sand carriers. As the result, this paper proposed the removal of hatch covers and be fitted by tarpaulin instead to protect water ingress into the holds. This can be done by making the holds smaller designed to be filled full until the top of the hold without exceeding loadline. Also, the bilge pumps should have the sufficient capacity of pumping water out in the early stage of sand-water mixture to wetted condition in approximately 1 hour and be operationally ready in every voyage conditions.

• Journal of Periodontal and Implant Science
• /
• v.45 no.4
• /
• pp.136-144
• /
• 2015

Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2). Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed. Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks. Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.34 no.1
• /
• pp.49-58
• /
• 2008

Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and ${\beta}-TCP$(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2. Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with $150{\sim}500{\mu}m$ diameter ${\beta}-TCP$ 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only ${\beta}-TCP$ was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. Result: Grafting ${\beta}-TCP$ with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of ${\beta}-TCP$ (8,12 week), even show less new bone formation than autogenous bone. Conclusion : The experimental study result that ${\beta}-TCP$ graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.

• International Journal of Control, Automation, and Systems
• /
• v.6 no.4
• /
• pp.534-543
• /
• 2008

To use the Global Positioning System (GPS) carrier phase measurement for precise positioning, the integer ambiguities at the early stage of most algorithms must be determined. Furthermore, if a precise positioning is to be applied to real time navigation, fast determination and validation methods for integer ambiguity are essential. In this paper, the Wald test that simultaneously determines and validates integer ambiguities is used with assistance of the Inertial Navigation System (INS) to improve its performance. As the Wald test proceeds, it assigns a higher probability to the candidate that is considered to be true at each time step. The INS information is added during the Wald test process. Large performance improvements were achieved in convergence time as well as in requiring fewer observable GPS satellites. To test the performance improvement of the Wald test with the INS information, experimental tests were conducted using a ground vehicle. The vehicle moved in a prescribed trajectory and observed seven GPS satellites. To verify the effect of the INS information on the Wald test, the convergence times were compared with cases that considered the INS information and cases that did not consider the INS information. The results show that the benefits of using the INS were emphasized as fewer GPS satellites were observable. The performance improvement obtained by the proposed algorithm was shown through the fast convergence to the true hypothesis when using the INS measurements.

• Korean Journal of Psychosomatic Medicine
• /
• v.8 no.1
• /
• pp.3-10
• /
• 2000

Objectives : The author wanted to summarize the psychiatric and social aspects of the patients with hepatitis B virus infection. Methods : The author reviewed all pertinent citations in the Medline database from 1966 to 1999. Results : Psychiatric problems in this population include delirium, psychotic disorder due to general medical condition(especially mania), anxiety, depression, adjustment disorder, alcohol abuse/dependence, and drug abuse/dependence. Social aspects of the patients with hepatitis B viral infection relate to the stigma of being a carrier, guilty feeling about infection, guilty feeling about increased family burden, impacts of having hepatitis on interpersonal relations, sexual difficulties, and job loss with increased financial burden, and health care worker's refusal. Conclusions : Appropriate early educational counseling interventions regarding the expected course and psychosocial intervention should be tailored to the sociocultural needs of special populations. Those interventions will increase compliance of treatment and prevent progression to hepatocellalar carcinoma from hepatitis.

• Structural Engineering and Mechanics
• /
• v.52 no.3
• /
• pp.613-632
• /
• 2014

In the early design stage of ships, the two most important structural analyses are performed to identify the structural capacity and safety. The first step is called global strength analysis (longitudinal strength analysis or hull girder strength analysis) and the second step is local buckling analysis (stiffened panel strength analysis). This paper deals with the ultimate strength performance of Arctic Sea Route-going commercial ships considering the effect of low temperature. In this study, two types of structural analyses are performed in Arctic sea conditions. Three types of ship namely oil tanker, bulk carrier and container ship with four different sizes (in total 12 vessels) are tested in four low temperatures (-20, -40, -60 and $-800^{\circ}C$), which are based on the Arctic environment and room temperature ($20^{\circ}C$). The ultimate strength performance is analysed with ALPS/HULL progressive hull collapse analysis code for ship hulls, then ALPS/ULSAP supersize finite element method for stiffened panels. The obtained results are summarised in terms of temperature, vessel type, vessel size, loading type and other effects. The important insights and outcomes are documented.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.36 no.3
• /
• pp.161-171
• /
• 2010

Introduction: This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and $\beta-TCP$ as a carrier in rabbits’mandible. Materials and Methods: The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits ; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, $\beta-TCP$P only was grafted, and in the experimental group 2, rhBMP-2 soaked in $\beta-TCP$ was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy. Results: In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of $\beta-TCP$ were observed. Conclusion: rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.