Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Morphometric analysis on bone formation effect of $\beta-TCP$ and rhBMP-2 in rabbit mandible

토끼의 하악골에서 $\beta-TCP$ 와 rhBMP-2의 골형성 효과에 대한 형태계측학적 연구

  • Kim, Kyu-Nam (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Yang, Jung-Eun (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Jang, Jea-Won (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Sasikala, Balaraman (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Wang, Beng (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Kim, Il-Kyu (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University)
  • 김규남 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 양정은 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 장재원 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 사시카라 바라라만 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 왕붕 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 김일규 (인하대학교 의과대학 부속병원 구강악안면외과)
  • Received : 2010.03.10
  • Accepted : 2010.05.04
  • Published : 2010.06.30
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Abstract

Introduction: This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and $\beta-TCP$ as a carrier in rabbits’mandible. Materials and Methods: The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits ; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, $\beta-TCP$P only was grafted, and in the experimental group 2, rhBMP-2 soaked in $\beta-TCP$ was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy. Results: In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of $\beta-TCP$ were observed. Conclusion: rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.

Keywords

References

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