Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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The BMPs expression and histomorphometric study of ${\beta}-TCP$ / rhBMP-2 Grafting on the rabbit cranial bone defects

  • Lim, Byung-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Jeon, Jae-Yoon (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Park, Chang-Joo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Im, Jae-Jung (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Hwang, Kyung-Gyun (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine, Hanyang University)
  • Published : 2008.02.29
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Abstract

Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and ${\beta}-TCP$(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2. Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with $150{\sim}500{\mu}m$ diameter ${\beta}-TCP$ 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only ${\beta}-TCP$ was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis. Result: Grafting ${\beta}-TCP$ with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of ${\beta}-TCP$ (8,12 week), even show less new bone formation than autogenous bone. Conclusion : The experimental study result that ${\beta}-TCP$ graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.

Keywords

References

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