• The Korean Journal of Physiology and Pharmacology
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• 제18권3호
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• pp.191-200
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• 2014

We investigated the anxiolytic-like activity of ${\alpha}$-asarone (AAS) from Acorus gramineus in an experimental rat model of anxiety induced by repeated administration of the exogenous stress hormone corticosterone (CORT). The putative anxiolytic effect of AAS was studied in behavioral tests of anxiety, such as the elevated plus maze (EPM) test and the hole-board test (HBT) in rats. For 21 consecutive days, male rats received 50, 100, or 200 mg/kg AAS (i.p.) 30 min prior to a daily injection of CORT. Dysregulation of the HPA axis in response to the repeated CORT injections was confirmed by measuring serum levels of CORT and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Daily AAS (200 mg/kg) administration increased open-arm exploration significantly in the EPM test, and it increased the duration of head dipping activity in the HBT. It also blocked the increase in tyrosine hydroxylase (TH) expression in the locus coeruleus (LC) and decreased mRNA expression of brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, in the hippocampus. These results indicated that the administration of AAS prior to high-dose exogenous CORT significantly improved anxiety-like behaviors, which are associated with modification of the central noradrenergic system and with BDNF function in rats. The current finding may improve understanding of the neurobiological mechanisms responsible for changes in emotions induced by repeated administration of high doses of CORT or by elevated levels of hormones associated with chronic stress. Thus, AAS did exhibit an anxiolytic-like effects in animal models of anxiety.

• 한국정보통신학회논문지
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• 제10권3호
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• pp.467-472
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• 2006

본 논문은 BLDC 서보 모터를 채 택하는 복합연막탄 선회구동 장치를 제어하기 위한 정밀 시스템설계 및 위치 제어 알고리즘 개발을 목적으로 하고 있다. 복합연막탄은 상대편 전차의 시계를 가리는 역할을 해야 하므로 짧은 시간 안에 상대방의 존재를 감지하고 연막탄을 발사할 수 있는 빠른 응답성이 요구된다. 따라서 $300[Hz]\sim500[Hz]$의 빠른 전류 응답 능력을 보이는 시스템 설계가 제안된다. 그리고 연산 속도의 증가를 위해 MIX-MAX PWM기법이 사용된다. 발사각 제어를 위해서는 지령 속도 궤적의 적분치를 활용하는 정밀 위치제어 알고리즘을 제안한다. 또한, 이러한 특성들이 전류, 전압, 위치, 속도 등의 파라미터 그래프를 보여주는 PC 반의 프로그램에 의해 모니터링 되고 평가된다. 부동소숫점 방식의 고성능 DSP인 TMS320VC33을 사용하여 제어기를 구성하였으며 PWM 발생부는 CPLD인 EPM7128을 사용하여 구현하였다.

• Biomolecules & Therapeutics
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• 제21권4호
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• pp.313-322
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• 2013

Previous studies have demonstrated that repeated administration of the exogenous stress hormone corticosterone (CORT) induces dysregulation in the hypothalamic-pituitary-adrenal (HPA) axis and results in depression and anxiety. The current study sought to verify the impact of catechin (CTN) administration on chronic CORT-induced behavioral alterations using the forced swimming test (FST) and the elevated plus maze (EPM) test. Additionally, the effects of CTN on central noradrenergic systems were examined by observing changes in neuronal tyrosine hydroxylase (TH) immunoreactivity in rat brains. Male rats received 10, 20, or 40 mg/kg CTN (i.p.) 1 h prior to a daily injection of CORT for 21 consecutive days. The activation of the HPA axis in response to the repeated CORT injections was confirmed by measuring serum levels of CORT and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Daily CTN administration significantly decreased immobility in the FST, increased open-arm exploration in the EPM test, and significantly blocked increases of TH expression in the locus coeruleus (LC). It also significantly enhanced the total number of line crossing in the open-field test (OFT), while individual differences in locomotor activities between experimental groups were not observed in the OFT. Taken together, these findings indicate that the administration of CTN prior to high-dose exogenous CORT significantly improves helpless behaviors, possibly by modulating the central noradrenergic system in rats. Therefore, CTN may be a useful agent for the treatment or alleviation of the complex symptoms associated with depression and anxiety disorders.

• 한국식품과학회지
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• 제50권2호
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• pp.244-247
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• 2018

유세포분석기, 형광현미경 계수법과 현재 주로 활용하는 숙주감염에 의한 용균반검사법에 의한 박테리오파지를 계수하는 방법을 Weissella cibaria 박테리오파지로 비교 연구하였다. 상관관계 분석은 피어슨 상관 통계분석, 회귀분석, 차이검정등으로 하였다. 12개의 시료로 유세포분석기와 용균반검사에 의한 계수법을 비교한 결과는 피어슨 분석으로는 96.7%의 상관관계(상관값 r=0.967, $R^2=0.9352$)와 차이검정은 1.063을 보였다. 형광현미경과 용균반검사에 의한 계수법 비교결과는 피어슨 분석으로는 99.0% (상관값 r=0.990, $R^2=0.9811$) 상관관계와 차이검정은 1.605이였다. 그러므로 유세포분석기와 형광현미경 계수법은 용균반검사 계수법과 같이 W. cibaria 박테리오파지 계수에 적합할 것으로 사료된다.

• 동의생리병리학회지
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• 제24권3호
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• pp.476-483
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• 2010

The present study was performed to investigate the putative anxiolytic-like effects of the aqueous extract of the roots of Scrophularia buergeriana (SB-W) using elevated plus-maze (EPM) and hole-board apparatus in mice. SB-W was orally administered at doses of 50, 100, 200 or 400 mg/kg to ICR mice, 1 h before the behavioral evaluation. Control group were administered with an equal volume of saline, and positive control group with buspirone (2 mg/kg, i.p.). The administration of SB-W significantly increased the percentage of time spent in open arms and entries into the open arms of the EPM compared with saline-treated control group (P < 0.05). Futhermore, those anxiolytic-like activities of SB-W were antagonized by flumazenil (a $GABA_A$ antagonist, 10 mg/kg), but not by WAY-100635 (a 5-$HT_{1A}$ antagonist, 0.3 mg/kg). Moreover, there were no changes in the locomotor activity and myorelaxant effects in any group compared with saline-treated control group. In the hole-board test, the administration of SB-W (200 and 400 mg/kg) significantly increased the number of head-dipping compared with saline-treated control group (P < 0.05). Therefore, these findings suggest that Scrophularia buergeriana promotes the anxiolytic-like activity mediated by GABAergic nervous system in mice.

• Biomolecules & Therapeutics
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• 제22권3호
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• pp.213-222
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• 2014

Abnormal adaptation of the stress-response system following traumatic stress can lead to alterations in the hypothalamic-pituitaryadrenal (HPA) axis that may contribute to the development of post-traumatic stress disorder (PTSD). The present study used several behavioral tests to investigate the anxiolytic-like and antidepressant activity of L-tetrahydropalmatine (L-THP) in an experimental rat model of anxiety and depression induced by single prolonged stress (SPS), an animal model of PTSD. Male rats were treated intraperitoneally (i.p.) with vehicle or varied doses of THP 30 min prior to SPS for 8 consecutive days. Daily THP (50 mg/kg) administration significantly increased the number and duration of open arm visits in the elevated plus maze (EPM) test, reduced the anxiety index, increased the risk assessment, and increased the number of head dips over the borders of the open arms after SPS. THP was also associated with increased time spent at the center of the open field, reduced grooming behaviors in the EPM test, and reduced time spent immobile in the forced swimming test (FST). It also blocked the decrease in neuropeptide Y (NPY) and the increase in corticotrophin-releasing factor (CRF) expression in the hypothalamus. This is the first study to determine that THP exerts pronounced anxiolytic-like and antidepressant effects on the development of the behavioral and biochemical symptoms associated with PTSD, indicating its prophylactic potential. Thus, THP reversed several behavioral impairments triggered by the traumatic stress of SPS and is a potential non-invasive therapeutic intervention for PTSD.

• 대한한방내과학회지
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• 제36권4호
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• pp.507-517
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• 2015

The bark of Magnolia officinalis has been used in traditional oriental medicine to treat a variety of mental disorders including anxiety and depression. The purpose of this study was to examine the effect of M. officinalis ethanol extract on stress-induced alterations in learning and cognitive function using a passive avoidance test (PAT) and also on anxiety-related behavior using the elevated plus-maze test (EPM) in female rats . The degree of Tyrosine hydroxylase (TH) in the region of the ventral tegmental area (VTA) and the locus coeruleus (LC) was measured using an immunohistochemical method. Corticosterone concentrations in serum were also measured. The ethanol extract from Magnolia officinalis was orally administered to female rats 30 minutes before evaluating their immobilization stress and anxiety-related behavior using an elevated plus-maze test and a passive avoidance test. Time spent in the open arms of the EPM increased in the M. officinalis-treated group compared with that of the saline-treated control group. In the passive avoidance test, the memory and cognitive function improved in the M. officinalis extract-treated group. M. officinalis extracts reduced elevated corticosterone concentrations in serum. Also, stress-induced TH increases were suppressed in the M. officinalis extract-treated group in the LC and the VTA region. These results suggest that M. officinalis might prove to be an effective anxiolytic anti-stress agent.

• 한국정보통신학회논문지
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• 제16권9호
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• pp.1884-1891
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• 2012

본 논문에서는 sliding상관기를 적용한 단문 메세지 서비스를 위한 대역확산 송수신기를 설계하고 대역확산 수신기에서 필수적인 PN코드 동기회로에 대한 성능을 분석하였다. 대역확산 시스템에 대한 이론적인 분석과 대역확산 수신기에 있어서 중요한 PN 코드 동기 회로에 대한 분석을 토대로 PN 코드 발생기, 클럭 발생을 위한 분주회로, 수신기에서의 PN 코드의 상관을 위한 슬라이딩 상관기 등을 Altera사의 칩 EPM7064 SLC44-10을 사용하여 FPGA화하였으며 디지털 설계가 용이하지 않은 주변회로인 슬라이딩 상관기에 필요한 PN코드 지연 클럭 발생회로, 동기 스위치제어회로, 데이터복조회로를 설계하여 전체적인 송수신기회로를 설계하였다. 설계된 회로를 실험을 통하여 송수신기의 성능을 평가 관찰하였다. 특히, 수신기에 있어서 역 확산을 위한 PN 신호의 동기과정의 성능 즉, 동기가 이루어 졌을 때의 동기 탐색/유지신호와 동기가 이루어지지 않았을 때의 게이트 지연시간으로 인한 동기 탐색/유지신호등의 결과를 통해 성능을 평가하였다. 슬라이딩 상관기의 경우 코드 동기를 위한 시간이 송수신 PN 코드의 불확정성이 클 경우 상당히 큼을 알 수 있었다.

• 한국농공학회지
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• 제25권2호
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• pp.42-53
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• 1983

In Korea, the demand for water is increasing greatly due to Korea's raqid economic progress which is similar to Japan's. A correct estimation of the runoff factors is the question that must be settled first to establish the appropritae plans for water use and water resources. of these plans the estimation of catchment evapotranspiration for every river basin is the subject of the most importance. It is impossible theoretically to measure evapotranspiration directly, because it is an at mospheric translatory phenomenon. Many approaches have been devised to estimate evapotranspiration, but each of these methods estimates from information taken from a specified point, and these methods are considered incomplete for estimating catchment evapotranspiration. In this paper, the seasonal evapotranspiration estimating method that was proposed by Linsly and was applied in the Kamigamo exprimental basin (subjected to Kyoto Univ.) by Takase et al, was used for the Geum river which is the main river in Korea. Conclusion of experiment. 1) The average annual Ec in this river basin from 1966 to 1972 was 470mm. That is considered appropriate since the average value for the six other large river basin in korea was 485mm. 2) The Ec/Ep and Ec/Epm ratios were 0.43 and 0.52, respectively (Ec : estimated evapotranspiration by water balance method, Ep : average pan evaporation, Epm : evaporation by Penman method). The seasonal Ec/Ep ratios were : 0.4 in spring, 0.6 in summer, 0.4 in autumn and 0.2 in winter. These are rather small when compared to Japan's or England's. 3) The reason for this was that the precipitational difference in wet and dry seasons were greater, an there was not sufficient soil moisture harmonize with the evapotranspiration capacity in the dry season, and that evapotranspiration was small due to the numerous barren mountains.

• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.357-366
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• 2016

Pro-inflammatory cytokine and brain-derived neurotrophic factor (BDNF) are modulated in post-traumatic stress disorder (PTSD). This study investigated the effects of ibuprofen (IBU) on enhanced anxiety in a rat model of PTSD induced by a single prolonged stress (SPS) procedure. The effects of IBU on inflammation and BDNF modulation in the hippocampus and the mechanisms underlying for anxiolytic action of IBU were also investigated. Male Sprague-Dawley rats were given IBU (20 or 40 mg/kg, i.p., once daily) for 14 days. Daily IBU (40 mg/kg) administration significantly increased the number and duration of open arm visits in the elevated plus maze (EPM) test, reduced the anxiety index in the EPM test, and increased the time spent in the center of an open field after SPS. IBU administration significantly decreased the expression of pro-inflammatory mediators, such as tumor necrosis $factor-{\alpha}$, $interleukin-1{\beta}$, and BDNF, in the hippocampus, as assessed by reverse transcription-polymerase chain reaction analysis and immunohistochemistry. These findings suggest that IBU exerts a therapeutic effect on PTSD that might be at least partially mediated by alleviation of anxiety symptoms due to its anti-inflammatory activity and BDNF expression in the rat brain.