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Anxiolytic-like Effects of Scrophularia buergeriana Miquel Using the Elevated Plus-Maze in Mice : Involvement of GABAergic Nervous System  

Choi, Yun-Hee (Department of Oriental Health Science, Wonkwang Digital University)
Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.3, 2010 , pp. 476-483 More about this Journal
Abstract
The present study was performed to investigate the putative anxiolytic-like effects of the aqueous extract of the roots of Scrophularia buergeriana (SB-W) using elevated plus-maze (EPM) and hole-board apparatus in mice. SB-W was orally administered at doses of 50, 100, 200 or 400 mg/kg to ICR mice, 1 h before the behavioral evaluation. Control group were administered with an equal volume of saline, and positive control group with buspirone (2 mg/kg, i.p.). The administration of SB-W significantly increased the percentage of time spent in open arms and entries into the open arms of the EPM compared with saline-treated control group (P < 0.05). Futhermore, those anxiolytic-like activities of SB-W were antagonized by flumazenil (a $GABA_A$ antagonist, 10 mg/kg), but not by WAY-100635 (a 5-$HT_{1A}$ antagonist, 0.3 mg/kg). Moreover, there were no changes in the locomotor activity and myorelaxant effects in any group compared with saline-treated control group. In the hole-board test, the administration of SB-W (200 and 400 mg/kg) significantly increased the number of head-dipping compared with saline-treated control group (P < 0.05). Therefore, these findings suggest that Scrophularia buergeriana promotes the anxiolytic-like activity mediated by GABAergic nervous system in mice.
Keywords
Anxiety; Scrophularia buergeriana; Elevated plus-maze; WAY 100635; Flumazenil;
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