• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5579-5587
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• 2013

Research indicates that a small population of cancer cells is highly tumorigenic, endowed with the capacity for self-renewal, and has the ability to differentiate into cells that constitute the bulk of tumors. These cells are considered the "drivers" of the tumorigenic process in some tumor types, and have been named cancer stem cells (CSC). Epithelial-mesenchymal transition (EMT) appears to be involved in the process leading to the acquisition of stemness by epithelial tumor cells. Through this process, cells acquire an invasive phenotype that may contribute to tumor recurrence and metastasis. CSC have been identified in human head and neck squamous cell carcinomas (HNSCC) using markers such as CD133 and CD44 expression, and aldehyde dehydrogenase (ALDH) activity. Head and neck cancer stem cells reside primarily in perivascular niches in the invasive fronts where endothelial-cell initiated events contribute to their survival and function. Clinically, CSC enrichment has been shown to be enhanced in recurrent disease, treatment failure and metastasis. CSC represent a novel target of study given their slow growth and innate mechanisms conferring treatment resistance. Further understanding of their unique phenotype may reveal potential molecular targets to improve therapeutic and survival outcomes in patients with HNSCC. Here, we discuss the state-of-the-knowledge on the pathobiology of cancer stem cells, with a focus on the impact of these cells on head and neck tumor progression, metastasis and recurrence due to treatment failure.

• 한국콘텐츠학회논문지
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• 제13권2호
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• pp.341-347
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• 2013

1급 응급구조사는 인체에 대한 해부학적 구조와 기능에 대한 이해를 바탕으로 정확한 임상술기를 수행해야 한다. 이러한 교육을 위해 응급구조(학)과 학생들은 기초의학 분야중 하나인 인체해부학을 이수해야 하며 카데바(cadaver)를 이용한 해부실습 교육이 통합적으로 필요하다. 따라서 본 연구는 응급구조(학)과 학생 255명에게 실시된 카데바를 이용한 해부학 실습의 효과를 조사 하였다. 그 결과 교육 만족도는 5점 척도 중 평균 4.50점으로 나타났으며, 주제별 시간의 적정도는 평균 3.61점이었고, 교육에 대한 이해도는 평균점으로 나타났다. 또한 93.3%의 학생이 재참여 혹은 다른 학생에게 추천할 의사가 있다고 응답하였고 학년별로 이해도 만족도 시간 적정도에 있어 통계적으로 유의한 차이가 있었다 (P<.05). 결론적으로, 추후 학년별 맞춤형 카데바 해부실습 프로그램에 대한 연구가 지속되어야 할 것이고 고학년은 실습 시간을 늘리고 육안해부학(gross anatomy) 뿐만 아니라 임상술기를 접목한 임상해부학(clinical anatomy)에 대한 실습이 통합적으로 진행되어야 함을 제언하는 바이다.

• 대한의생명과학회지
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• 제19권4호
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• pp.295-302
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• 2013

Some of the pituitary adenomas are invasive and spread into neighboring tissues. In previous studies, the invasion of pituitary adenomas is thought to be associated with epithelial-mesenchymal transition (EMT). In addition to that, we thought that mesenchymal stem cells (MSCs) exist in relevant microenvironment in pituitary adenoma. However, it has been little known about the existence of MSCs from pituitary adenoma. So we investigated whether mesenchymal stem-like cells (MSLCs) can be isolated from the pituitary adenoma specimen. We isolated and cultured candidate MSLCs from the fresh pituitary adenoma specimen with the same protocols used in culturing bone marrow derived MSCs (BM-MSCs). The cultured candidate MSLCs were analyzed by fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Candidate MSLCs were exposed to mesenchymal differentiation conditions to determine the mesenchymal differentiation potential of these cells. To evaluate the tumorigenesis of candidate MSLCs from pituitary adenoma, we implanted these cells into the brain of athymic nude mice. We isolated cells resembling BM-MSCs named pituitary adenoma stroma mesenchymal stem-like cells (PAS-MSLCs). PAS-MSLCs were spindle shaped and had adherent characteristics. FACS analysis identified that the PAS-MSLCs had a bit similar surface markers to BM-MSCs. Isolated cells expressed surface antigen, positive for CD105, CD75, and negative for CD45, NG2, and CD90. We found that these cells were capable of differentiation into adipocytes, osteocytes and chondrocytes. Tumor was not developed in the nude mice brains that were implanted with the PAS-MSLCs. In this study, we showed that MSLCs can be isolated from a pituitary adenoma specimen which is not tumorigenic.

• 한국산학기술학회논문지
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• 제16권10호
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• pp.6647-6654
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• 2015

본 연구는 응급구조학과 학생대상 한국판 시뮬레이션 경험 만족도 측정도구 분석을 목적으로 응급구조학과 학생 총 111명을 대상으로 ACLS시뮬레이션 실습교육 후 학생들이 느끼는 만족도를 측정 후 분석하였다. 또한 시뮬레이션 교육에 대한 호감도, 필요성, 전문심장소생술 이론 성적이 시뮬레이션 실습교육 만족도에 미치는 영향 및 상관관계를 확인하고자 한다. 측정된 만족도는 일원배치분산분석을 통하여 분석하였으며, 그 결과 대부분의 학생들이 높은 만족도를 나타냈다. 시뮬레이션 교육에 대한 호감도 및 필요도가 높을수록 만족도 측정도구의 세 가지 요인 모두에서 높게 나타났으나, ACLS이론 성적과 만족도의 상관관계는 성립되지 않았다. 향후 ACSL 시뮬레이션을 통한 교육법은 유지되어야 하며 지속적으로 발전해야 한다.

• 한국산업보건학회지
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• 제27권3호
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• pp.170-179
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• 2017

Objectives: This study aims to investigate the occupational radiation exposures of emergency medical technicians(EMTs) in emergency medical centers in Korea. The results will provide a basis for developing prevention programs to minimize adverse health effects relating to radiation exposure among emergency medical technicians working in this area. Methods: Radiation exposure doses were measured for twenty-two EMTs working in six emergency medical centers. Thermo Luminescent Dosimeters(TLD) were placed on three representative body parts, including chest, neck, and a finger. Measurements were conducted over the entire working hours of the participants for foor weeks. Dosimeters were analyzed according to a standard method by a KFDA-designated lab. Detection rate, annual radiation exposure dose, and relative levels to dose limit were derived based on the measured doses from the dosimeters. SPSS/Win 18.0 software(IBM, US) was used for statistical analysis. Results: Detection rates were 45.5%, 36.4%, and 45.5% for the dosimeters sampled from chest, neck, and a finger, respectively. The average annual doses were $2.39{\pm}3.44mSv/year$(range 0.38-10.0 mSv/year) for the chest, $2.72{\pm}3.05mSv/year$(2.00-11.34) for the neck, and $20.98{\pm}17.57mSv/year$(1.25-53.50) for the hand dose. The average annual eye dose was estimated to $3.61{\pm}2.37mSv/year$(1.50-8.34). The exposure dose levels of EMTs were comparable to those of radiologists, who showed relatively higher radiation dose among health care workers, as reported in another study. Conclusions: EMTs working in emergency medical centers are considered to be at risk of radiation exposure. Although the radiation exposure dose of EMTs does not exceed the dose limit, it is not negligible comparing to other professionals in health care sectors.

• Molecules and Cells
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• 제40권10호
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• pp.761-772
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• 2017

Glioblastoma is the most frequent and most aggressive brain tumor in adults. Solute carrier family 8 member 2 (SLC8A2) is only expressed in normal brain, but not present in other human normal tissues or in gliomas. Therefore, we hypothesized that SLC8A2 might be a glioma tumor suppressor gene and detected the role of SLC8A2 in glioblastoma and explored the underlying molecular mechanism. The glioblastoma U87MG cells stably transfected with the lentivirus plasmid containg SLC8A2 (U87MG-SLC8A2) and negative control (U87MG-NC) were constructed. In the present study, we found that the tumorigenicity of U87MG in nude mice was totally inhibited by SLC8A2. Overexpression of SLC8A2 had no effect on cell proliferation or cell cycle, but impaired the invasion and migration of U87MG cells, most likely through inactivating the extracellular signal-related kinases (ERK)1/2 signaling pathway, inhibiting the nuclear translocation and DNA binding activity of nuclear factor kappa B ($NF-{\kappa}B$), reducing the level of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (uPA)-its receptor (uPAR) system (ERK1/2-$NF-{\kappa}B$-MMPs/uPA-uPAR), and altering the protein levels of epithelial to mesenchymal transitions (EMT)-associated proteins E-cardherin, vimentin and Snail. In addition, SLC8A2 inhibited the angiogenesis of U87MG cells, probably through combined inhibition of endothelium-dependent and endothelium-nondependent angiogenesis (vascular mimicry pattern). Totally, SLC8A2 serves as a tumor suppressor gene and inhibits invasion, angiogenesis and growth of glioblastoma.

• 패션비즈니스
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• 제12권6호
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• pp.46-60
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• 2008

Lyocell is a regenerated cellulosic fiber manufactured by an environmentally friendly process. The major advantages of lyocell are the excellent drape forming property, the genuine bulkiness, smooth surface, and high dry/wet tenacities. However, one drawback of lyocell is its fibrillation property, which would degrade its aesthetic quality and lower the consumer satisfaction. In our previous studies, lyocell was treated with epichlorohydrin, a non-formalin based crosslinker, to reduce its fibrillation tendency. To investigate the changes of physical properties upon ECH-treatment, the hand characteristics of ECH-treated fabric were observed using KES-FB system and the 3D-virtual sewing image of the fabrics were obtained using 3D CAD simulation system in this study. Since epichlorohydrin(ECH) treatment was conducted in the alkaline medium, the weight reduction was observed in all treated lyocell. The treated lyocell became light, smooth and flexible in spite of ECH crosslinker application. LT and RT in tensile property upon the ECH treatment did not change significantly, however, EMT and WT in the tensile property increased. The significant decrease in bending rigidity was resulted in all ECH-treated lyocell, which is the result of the weight loss upon the alkali condition of ECH treatment. The bending rigidity increased again in the ECH 30% treated lyocell, however, the B value is still lower than the original. Therefore, the ECH-treated lyocell would be more stretchable and softer than the original. Shear rigidity was also decreased in all ECH-treated lyocell, which would result in more drape and body fitting when it is made as a garment. The ECH-treated fabric showed the softer smoother surface according to SMD value from KES evaluation. The virtual 3D sewing image of the ECH-treated lyocell did not show a significant change from that of the original except ECH 30% treated lyocell. ECH 30% treated lyocell showed a stiffer and more puckered image than the original.

• 한국학교보건학회지
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• 제22권2호
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• pp.147-156
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• 2009

Purpose: The purpose of this study was to analyze students' expectance demand towards high school physical education teaching. Methords : 392 participants randomly selected from one girls' middle school and one girl's high school in each of five tracts and then selected two classes from each of the schools. They were asked to respond to a simple questionnaire about their views on the present physical education teaching. Results : Results showed that about one third of the participants would not choose physical education if the program were offered as an optional subject. Both male and female students ranked psychomotor domain as the top priority among the various learning objectives. Male students favored soccer and basketball while their counterparts liked badminton and volleyball. Over three-fourths of the participants suggested that students' opinions should be considered when teachers plan their program. Conclusion : The results of this study recommended that physical education teaching should be designed with considerations for professional values, societal changes as well as students' expectance on the design of physical education.

• 대한안전경영과학회지
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• 제16권4호
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• pp.71-80
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• 2014

This study is performed to determine the priorites of the evaluation factors for the improvement of fire emergency medical service by increasing it's efficiency to protect the people's lives effectively, and by recognizing it's mid-long term development. Based on the output of the analysis, the critical evaluation factors of the fire emergency medical services to be improved shall be identified, and shall be intended to use strategically for increasing the efficiency of all the activity in the EMT service, and also for providing the basic date to product and manage the good quality services. To calculate the priority of the evaluation factor for each level as shown on this study, AHP(anaysis hierarchy process) with pairwise comparison method is applied. According to the priority analysis for the evaluation factors in main category (evaluation zone), 'medical service professional aspect' is recognized as highest one, and 'service infrastructure aspect', 'service management aspect' are indicated in order. According to the priority analysis for the evaluation factors in evaluation index, 'professional personal arrangement' in the service infrastructure aspect, 'appropriateness and timeliness of emergency medical care' in the medical service professional aspect, and 'clinical training' in the service management aspect are respectively recognized as most important factors.

• 한국보건간호학회지
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• 제14권2호
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• pp.332-341
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• 2000

Children's accident is a largely preventable public health problem. Little is known. however, about population-based incident and outcome of pediatric accident. From 1997.9 through 1998,8. admission data from emergency center in I city were collected. 1418 patient from 0 through 13 years of age were selected. All children with unintensional accidental problems were identified through coded sheet which categorizes epidemiologic characteristics. The specific purposes of this study are analysis about the characteristics of pediatric accidents. And it aims to produce the basic data necessary for accident prevention policy development. The results of this study were as follows; 1. The number of male children$(62.6\%)$ were higher than female children$(37.4\%)$ 2. The age group from 1 to 3 years represents the highest proportion$(45.4\%)$ of every accidents except on traffic accident. 3. The highest proportion of accident were as follows occured during the June-August$(34\%)$, Sunday$(22.6\%)$, and 17-21 p.m. $(37.2\%)$ 4. The main causes of accident include general trauma$(70.9\%)$, environmental accident$(l6.8\%)$. and traffic accident$(l2.1\%)$, 5. Preschool age group represents more than half$(65.4\%)$ of traffic accident. 6. environmental injury includes burns $(46.6\%)$, foreign body$(43.6\%)$, exposure to poisonous materials$(6.3\%)$. and bite(3.3) This results could be used to develope prevention programs and assist in accident prevention system development. And also these data substantiate that accident prevention program decrease safety-related injury rate in preschool age group must be concentrated on enhancing access to a system to have a significant effect. Furthermore, it is necessary for accident prevention. So several suggestions are described here: 1. Development of parent's educational program for accident prevention and safety education should be done actively. 2. Home safety surveillance system should be initiated. 3. The initiation of children's accident report system could be contribute the analysis and the reduction of accident.