• Journal of Veterinary Science
• /
• 제21권1호
• /
• pp.9.1-9.15
• /
• 2020

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

• 전기학회논문지P
• /
• 제63권3호
• /
• pp.183-188
• /
• 2014

Power conversion systems used in large gas turbine power plant can be divided into two main part. Because of the initial start-up characteristic of the gas turbine combustor, the gas turbine must be accelerated by starting device(LCI : Load Commutated Inverter) up to 10%~20% of rated speed to ignite it. In addition, the ECS(Excitation Control system) is used to control the rotor field current and reactive power in grid-connected synchronous generator. These two large power conversion systems are located in the same space(container) because of coordination control. Recently, many manufactures develop high speed controller based on function block available in the LCI and ECS with the newest power semiconductor. We also developed high speed controller based on function block to be using these two system and it meets the international standard IEC61131 as using real-time OS(VxWorks) and ISaGRAF. In order to install easily these systems at power plant, main controller, special module and IO module are used with high speed communication line other than electric wire line. Before initial product is installed on the site, prototype is produced and tests are conducted for it. The performance results of Integrated controller and application program(SFC, ECS) were described in this paper. The test results will be considered as the important resources for the application in future.

• 대한한방소아과학회지
• /
• 제34권1호
• /
• pp.37-47
• /
• 2020

Objectives After inducing dermatitis in 6-week-old mice, we tried to find out the effects of recovery in the damaged skin by administering genistein and palmitoylethanolamide (PEA) Methods The 6-week-old mice were divided into the control group (Ctrl), dermatitis causing group (AcDE), genistein-administered group after the onset of dermatitis (GsT), and PEA-administered group after the onset of dermatitis (PEAT). Seven mice were assigned to each group. Changes in the skin barrier were observed after three days of administration following the onset of dermatitis. Results In the GsT and PEAT, there was less skin damage compared to the AcDE, and the lowest skin damage showed in the GsT. The intensity of CB1 and CB2 expression was increased by 64% (CB1) and 39% (CB2) in the GsT, and 38% (CB1) and 28% (CB2) in the PEAT compared to the AcDE. The E-catherin positive reaction was decreased in the AcDE, while the E-catherin positive reaction was increased in the GsT (76%) and PEAT (34%). The p-JNK positive reaction was increased in the AcDE, while the p-JNK positive reaction was decreased in the GsT (60%) and PEAT (39%). Toxic Hepatopathy was not observed in the liver tissue of Genistein administered 3OGsT, and PEA administered 3OPEAT. Conclusions Genistein has recovery effect on dermatitis damage through active induction of the endocannabin system (ECS).

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2010년도 제35회 추계학술대회논문집
• /
• pp.628-635
• /
• 2010

냉각기계를 적용한 환경조절계통의 성능은 항공기의 비행환경에 따라 엔진의 운전상태가 달라지므로 환경조절장치로 취출되는 공기의 상태(온도와 압력)에 따라 급격히 바뀌게 된다. 즉, 항공기의 운전상태에 따라 환경조절장치는 운용성능을 100%만족하는 영역에서 운전될 수도 있고, 그렇지 않을 수도 있다. 그렇기 때문에, 일반적으로 냉각기계를 적용한 환경조절장치의 설계규격은 항공기에서 요구하는 개발규격보다 큰 설계여유를 갖도록 설계한다. 이로 인한 시스템적인 손실요인을 최소화하기 위하여 본 연구에서는 냉각기계형 환경조절장치에 상변화 물질의 적용가능성을 연구하였다. 그 결과 상변화 현상을 이용하여 환경조절장치의 출구온도를 일정온도 범위 이내로 제어하면, 현재 운용되는 냉각기계형 환경조절장치의 일반적인 성능여유보다 적은 성능여유에서도 환경조절장치에 요구되는 성능요구조건을 만족할 수 있는 것으로 분석되었다. 본 연구 결과 환경조절장치의 운용개념 변경을 통하여 환경조절장치의 시스템 적용성 향상을 기대할 수 있을 것으로 판단된다.

• 대한한방소아과학회지
• /
• 제34권4호
• /
• pp.22-30
• /
• 2020

Objectives This study was conducted to confirm the inhibitory effect of β-glucan on epithelial inflammation induced by atopic dermatitis through Endocannabinoid system (ECS) activity. Methods Six-week-old NC/Nga mice were divided into a control group (Ctrl), atopic dermatitis elicitation group (ADE), and a β-glucan-treated group (β-glucan treatment after atopy dermatitis elicitation, β-GT). After 3 weeks, CB1, CB2, and GPR55 were observed to confirm the regulation of ECS activity, and filaggrin in the stratum corneum and Kallikrein-related peptidase (KLK) 7 in the stratum corneum and protease activated receptor (PAR)-2 were observed to confirm the inhibition of the inflammation, Phosphorylated extracellular signal-related kinase (p-ERK), Phosphorylated mammalian target of rapamycin (p-mTOR), and E-Cadherin were observed to confirm microenvironmental regulation. Results β-GT was significantly increased in CB1, CB2, and GPR55 positive reactions compared to that of the ADE. In positive reaction of the filaggrin in the stratum corneum, β-GT was significantly increased than that of the ADE. For KLK7 positive and PAR2 positive, β-GT was significantly reduced compared to the ADE. The p-ERK-positive and p-mTOR-positive reactions were significantly reduced in β-GT than in ADE. E-cadherin positive reaction was significantly increased in β-GT than in ADE (All p < 0.01). Conclusions It was confirmed that β-glucan has the effect of inhibiting the epithelium induced by atopic dermatitis through the ECS activity.

• 한국항공우주학회지
• /
• 제40권11호
• /
• pp.934-939
• /
• 2012

본 연구는 무인항공기의 환경제어계통에 적용되는 PAO-공기 열교환기에 대한 성능검증에 관한 것으로, 계통에 대한 성능분석을 통해 열교환기 성능목표를 설정하고 상세설계와 정밀제작을 통해 목표성능에 부합되는 열교환기를 개발하였다. 개발된 PAO-공기 열교환기의 성능을 입증하기 위해 열교환기의 설계점 외에 다양한 시험조건에 대한 압력손실과 열전달 유용도, 총괄열전달계수 등에 대해 시험을 수행하였다. 수행된 결과를 바탕으로, 무인항공기용 환경제어계통에 적용된 PAO-공기 열교환기의 성능과 특성을 분석하여 개발적합성에 대해 검증하였다.

• Journal of Information Technology Applications and Management
• /
• 제10권1호
• /
• pp.111-125
• /
• 2003

This paper proposes a product data model that can express and enforce integrity constraints on product structure during engineering changes (ECs). The model adopts and extends an active object-oriented database model in order to Integrate IC data and their integrity constraints. Tightly integrated with product structure, It will enable designers to maintain and exchange consistent EC data throughout the product life cycle. In order to properly support operations for ECs, the model provides the data, operations, and Event-Condition-Action rules for nested ECs and simultaneous EC applications to multiple options. in addition, the EC objects proposed In the model integrate the data and Integrity constraints into a unified repository. This repository enables designers to access all EC data and integrity constraints through the product structure and relationships between EC objects. This paper also describes a prototype product data management system based on the proposed model In order to demonstrate its effectiveness.

• Advances in aircraft and spacecraft science
• /
• 제5권4호
• /
• pp.499-513
• /
• 2018

One of the major problems in glass fiber reinforced epoxy (GFRE) composite pipes is the durability under water absorption. This condition is generally recognized to cause degradations in strength and mechanical properties. Therefore, there is a need for an intelligent system for detecting the absorption rate and computing the mass of water absorption (M%) as a function of absorption time (t). The present work represents a new non-destructive evaluation (NDE) technique for detecting the water absorption rate by evaluating the dielectric properties of glass fiber and epoxy resin composite pipes subjected to internal hydrostatic pressure at room temperature. The variation in the dielectric signatures is employed to design an electrical capacitance sensor (ECS) with high sensitivity to detect such defects. ECS consists of twelve electrodes mounted on the outer surface of the pipe. Radius-electrode ratio is defined as the ratio of inner and outer radius of pipe. A finite element (FE) simulation model is developed to measure the capacitance values and node potential distribution of ECS electrodes on the basis of water absorption rate in the pipe material as a function of absorption time. The arrangements for positioning12-electrode sensor parameters such as capacitance, capacitance change and change rate of capacitance are analyzed by ANSYS and MATLAB to plot the mass of water absorption curve against absorption time (t). An analytical model based on a Fickian diffusion model is conducted to predict the saturation level of water absorption ($M_S$) from the obtained mass of water absorption curve. The FE results are in excellent agreement with the analytical results and experimental results available in the literature, thus, validating the accuracy and reliability of the proposed expert system.

• Biomolecules & Therapeutics
• /
• 제28권1호
• /
• pp.45-57
• /
• 2020

In the neurovascular unit, the neuronal and vascular systems communicate with each other. O₂ and nutrients, reaching endothelial cells (ECs) through the blood stream, spread into neighboring cells, such as neural stem cells, and neurons. The proper function of neural circuits in adults requires sufficient O₂ and glucose for their metabolic demands through angiogenesis. In a central nervous system (CNS) injury, such as glioma, Parkinson's disease, and Alzheimer's disease, damaged ECs can contribute to tissue hypoxia and to the consequent disruption of neuronal functions and accelerated neurodegeneration. This review discusses the current evidence regarding the contribution of oxygen deprivation to CNS injury, with an emphasis on hypoxia-inducible factor (HIF)-mediated pathways and Notch signaling. Additionally, it focuses on adult neurological functions and angiogenesis, as well as pathological conditions in the CNS. Furthermore, the functional interplay between HIFs and Notch is demonstrated in pathophysiological conditions.

• Journal of Biosystems Engineering
• /
• 제32권2호
• /
• pp.109-114
• /
• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.