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http://dx.doi.org/10.7778/jpkm.2020.34.4.22

Effect of β-glucan on Epithelial Inflammation Induced by Atopic Dermatitis through Endocannabinoid System Activity  

Seo, Il Bok (Dept. of anatomy, college of Korean Medicine, Semyung University)
Ahn, Sang Hyun (Dept. of anatomy, college of Korean Medicine, Semyung University)
Kim, Ki Bong (Dept. of Korean Pediatrics, Korean Medicine Hospital, Pusan National University)
Publication Information
The Journal of Pediatrics of Korean Medicine / v.34, no.4, 2020 , pp. 22-30 More about this Journal
Abstract
Objectives This study was conducted to confirm the inhibitory effect of β-glucan on epithelial inflammation induced by atopic dermatitis through Endocannabinoid system (ECS) activity. Methods Six-week-old NC/Nga mice were divided into a control group (Ctrl), atopic dermatitis elicitation group (ADE), and a β-glucan-treated group (β-glucan treatment after atopy dermatitis elicitation, β-GT). After 3 weeks, CB1, CB2, and GPR55 were observed to confirm the regulation of ECS activity, and filaggrin in the stratum corneum and Kallikrein-related peptidase (KLK) 7 in the stratum corneum and protease activated receptor (PAR)-2 were observed to confirm the inhibition of the inflammation, Phosphorylated extracellular signal-related kinase (p-ERK), Phosphorylated mammalian target of rapamycin (p-mTOR), and E-Cadherin were observed to confirm microenvironmental regulation. Results β-GT was significantly increased in CB1, CB2, and GPR55 positive reactions compared to that of the ADE. In positive reaction of the filaggrin in the stratum corneum, β-GT was significantly increased than that of the ADE. For KLK7 positive and PAR2 positive, β-GT was significantly reduced compared to the ADE. The p-ERK-positive and p-mTOR-positive reactions were significantly reduced in β-GT than in ADE. E-cadherin positive reaction was significantly increased in β-GT than in ADE (All p < 0.01). Conclusions It was confirmed that β-glucan has the effect of inhibiting the epithelium induced by atopic dermatitis through the ECS activity.
Keywords
${\beta}$-glucan; Atopic Dermatitis; Endocannabinoid system (ECS); filaggrin;
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Times Cited By KSCI : 6  (Citation Analysis)
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