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http://dx.doi.org/10.5307/JBE.2007.32.2.109

Development of Culture System for Masspropagation and Acclimatization of Tissue Cultured Plantlets  

Han, K.S. (National Institute of Agricultural Engineering, RDA)
Heo, J.W. (National Institute of Agricultural Engineering, RDA)
Kim, S.C. (National Institute of Agricultural Engineering, RDA)
Lee, Y.B. (National Institute of Agricultural Engineering, RDA)
Kim, S.C. (National Institute of Agricultural Engineering, RDA)
Im, D.H. (National Institute of Agricultural Engineering, RDA)
Choi, H.G. (National Institute of Agricultural Engineering, RDA)
Publication Information
Journal of Biosystems Engineering / v.32, no.2, 2007 , pp. 109-114 More about this Journal
Abstract
In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.
Keywords
Seed potato; Plantlet; Plant tissue culture; Forced ventilation; Masspropagation; Growth and development;
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