• 한국수산과학회지
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• 제30권4호
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• pp.614-619
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• 1997

해양오염의 진단을 위한 생화학적 오염지표 설정의 기초연구의 일환으로서 오염의 정도가 심각한 서해산 도다리 (Pleuronichthys cornutus)의 뇌 및 근육중의 acetylcholinesterase (AChE) 및 butyrylcholinesterase (BChE)의 활성, 그리고 lactate dehydrogenase(LDH)의 활성을 분석 평가하였다. 서해안 자연산 도다리의 뇌 및 근육중의 AChE의 활성은 $7,708.2{\pm}549.7\~8,872.7{\pm}515.1\;unit/min/mg$ protein (brain) 및 $4,071.2{\pm}647.9\~6,0245{\pm}493.7unit/min/mg$ protein (muscle)로서 대조군으로 사용한 동해안 포항의 자연산 도다리의 뇌 및 근육중의 AChE의 활성 ($11,168.9{\pm}822\;unit/min/mg\;protein\;(brain)$ 및 $6,812.2{\pm}533.0\;unit/min/mg\;protein\;(muscle);\;100\%$) 대비 각각 $69.1\~79.4\%$ 및 $59.8\~88.4\%$로서 대조군 대비 $20\~30\%$ (뇌) 및 $10\~40\%$ (근육) 정도나 유의적으로 저하되었다. 또한 서해안 자연산 도다리의 뇌 및 근육중의 BChE의 활성은 $274.1{\pm}51.2\~362.1{\pm}27.3\;unit/min/mg\;protein(brain)$ 및 $1,535.4{\pm}301.4\~1,854.7{\pm}250.7\;unit/min/mg\;protein(muscle)$로서 대조군으로 사용한 동해안 포항의 자연산 도다리의 뇌 및 근육중의 BChE의 활성 ($390.2{\pm}32.5\;unit/min/mg\;protein\;(brain)$ 및 $2,732.5{\pm}320.1\;unit/min/mg\;protein\;(muscle);\;100\%$) 대비 $92.8\~70.3\%$ (뇌) 및 $56.2\~67.9\%$ (근육)으로서, 대조군 대비 $10\~30\%$ 및 $35\~45\%$나 유의적으로 저하되었다. 서해안의 자연산 도다리의 혈청중의 LDH의 활성은 격포산 도다리를 제외하고 $0.075{\pm}0.030\~0.078{\pm}0.030\;unit/m\ell\;serum$로서 대조군으로 사용한 동해안 포항의 자연산 도다리의 혈액중의 LDH의 활성 $(0.105{\pm}0.030\;unit/m\ell\;serum;\;100\%)$ 대비 약 $30\%$정도나 유의적으로 저하하였다. 이상의 실험결과에서 볼 때 서해안의 자연산 도다리의 서식환경인 서해안의 오염도 상당히 심각할 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권6호
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• pp.391-397
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• 2010

E2F transcription factors and their target genes have been known to play an important role in cell growth control. We found that curcumin, a polyphenolic phytochemical isolated from the plant Curcuma longa, markedly suppressed E2F4 expression in HCT116 colon cancer cells. Hydrogen peroxide was also found to decrease E2F4 protein level, indicating the involvement of reactive oxygen species (ROS) in curucmin-induced downregulation of E2F4 expression. Involvement of ROS in E2F4 downregulation in response to curcumin was confirmed by the result that pretreatment of cells with N-acetylcystein (NAC) before exposure of curcumin almost completely blocked the reduction of E2F4 expression at the protein as well as mRNA level. Anti-proliferative effect of curcumin was also suppressed by NAC which is consistent to previous reports showing curcumin-superoxide production and induction of poly (ADP-ribose) polymerase (PARP) cleavage as well as apoptosis. Expression of several genes, cyclin A, p21, and p27, which has been shown to be regulated in E2F4-dependent manner and involved in the cell cycle progression was also affected by curcumin. Moreover, decreased (cyclin A) and increased (p21 and p27) expression of these E2F4 downstream genes by curcumin was restored by pretreatment of cells with NAC and E2F4 overexpression which is induced by doxycycline. In addition, E2F4 overexpression was observed to partially ameliorate curcumin-induced growth inhibition by cell viability assay. Taken together, we found curcumin-induced ROS down-regulation of E2F4 expression and modulation of E2F4 target genes which finally lead to the apoptotic cell death in HCT116 colon cancer cells, suggesting that E2F4 appears to be a novel determinant of curcumin-induced cytotoxicity.

• 한국수산과학회지
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• 제29권4호
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• pp.464-473
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• 1996

쥐노래미의 단백질 요구량을 조사하기 위해 카제인과 북양어분을 단백질원으로 하여, 사료의 단백질 함량이 30, 40, 50, $60\%$가 되도록 조정한 4종의 실험사료와 사료의 적정 에너지 함량을 조사하기 위해 가용에너지/단백질 (EP) 비가 9, 10, 11, 12인 4종의 실험 사료를 각각 설계하여 사육실험을 실시하였다. 29g 전후의 쥐노래미를 2개월간 사료의 단백질 함량별로 2반복으로 사육 실험한 결과, 증체율 및 사료효율은 모두 단백질 $50\%$ 사료에서 가장 좋았고, 단백질 $60\%$ 사료에서 성장효과는 오히려 감소하여 4개 실험구 중에서 가장 낮은 값을 나타내었다 (P<0.05). 수학적인 방법으로 이차회귀곡선상에서 증체율을 지표로 하여 단백질요구량을 추정한 결과 최대 단백질 요구량이 $45\%$로 나타났다. 일일사료섭취율은 실험구간에 서로 차이가 없었으나, 단백질섭취율은 사료의 단백질 함량이 증가됨에 따라 증가하는 경향을 보였으며, 단백질효율은 사료 단백질 함량이 증가될수록 유의하게 감소하였다(P<0.05). 가장 성장이 좋았던 실험구의 일일단백질섭취율이 어체중 100g 당 1.7g 이였으며, 이차회귀곡선상에서는 일일단백질 요구량이 어체중 100g당 1.5g으로 나타났다. 전어체의 수분함량은 사료 단백질 함량 $40\~60\%$구간에서는 차이가 없었으나, 사료 단백질 $30\%$구는 $40\%$구보다 유의하게 낮은 값을 보였다(P<0.05). 단백질과 회분 함량은 실험구간에 차이가 없었으며, 지질 함량은 단백질 $30\%$ 실험구가 가장 높았고, $40\%$ 실험구가 가장 낮았다(P<0.05) E/P 비가 다른 사료로 사육 실험한 결과, 증체율, 사료효율 및 단백질효율이 E/P 비가 12인 실험구가 다른 실험구보다 높은 값을 보였다. 전어체의 수분, 단백질 및 회분 함량은 사료의 에너지 함량에 영향을 받지 않았으나, 지질 함량은 에너지가 높은 사료인 E/P 비 11과 12 사료에서 유의적으로 높은 값을 보였다. 본 실험 결과로부터 평균체중 100g 이하의 성장기 쥐노래미의 단백질 요구량은 $45\~50\%$, 일일단백질 요구량은 어체중 100g 당 $1.5\~1.7g$으로 추정되며, 쥐노래미 사료의 적정 E/P 비는 12 전후가 적당할 것으로 생각된다.

• 대한의생명과학회지
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• 제29권4호
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• pp.287-295
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• 2023

Amifostine was developed to protect cells, but it is known to induce cytotoxicity and apoptosis, and the exact mechanism is unknown. In this study, we investigated how the DNA mismatch repair (MMR) system interacts with p53 to prevent apoptosis, cell cycle arrest, and cytoprotective effects induced by amifostine. HCT116 colon cancer cells sublines HCT116/p53+,HCT116/p53+, HCT116/p53-, HCT116/E6 and HCT116+ch3/E6 cells were used for evaluation. Amifostine induced G1 arrest and increased toxicity two-fold in p53- cells regardless of MMR expression. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Amifostine induced the expression of p21 protein in both p53+ and p53- cells. As for apoptosis, compared to p53- cells, p53+ cells showed 3.5~4.2 times resistance to amifostine-induced apoptosis. HCT116+E6 with both p53 and MMR loss showed maximum apoptosis at 48 h, and HCT116+ch3/E6HCT116+ch3/E6 with p53 loss showed maximum apoptosis at 24 h. As a result, it was confirmed through in vitro experiments that amifostine-induced G1 cell cycle arrest and apoptosis are mediated through a pathway dependent on MMR and p53 protein.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.207-213
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• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.1033-1039
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• 2006

The metabolic response of Labeo rohita to thermal acclimation was assessed. Advanced fingerlings of L. rohita (average weight $31{\pm}1.4g$) were acclimated to 31, 33 and $36^{\circ}C$ compared with ambient temperatures ($26^{\circ}C$) for 30 days and different enzymes associated with stress response were estimated. Glycolytic enzyme-Lactate dehydrogenase, (LDH, E.C.1.1.1.27), TCA cycle enzyme-Malate dehydrogenase (MDH, E.C.1.1.1.37), Protein metabolizing enzymes-Aspartate amino transferase (AST, E.C.2.6.1.1) and Alanine amino transferase (ALT, E.C.2.6.1.2) of liver, gill and muscle, Gluconeogenic enzymes-Fructose 1,6 Bi phosphatase (FBPase, E.C. 3.1.3.11) and Glucose 6 phosphatase (G6Pase, E.C. 3.1.3.9) of liver and kidney were significantly (p<0.05) different with increasing acclimation temperatures. Heat Shock Protein-70 (HSP-70) was expressed in increasing intensity at 31, 33 and $36^{\circ}C$ but was not expressed at $26^{\circ}C$. Results suggest that higher acclimation temperatures enhance metabolism and L. rohita maintains homeostasis between $26-36^{\circ}C$ via an acclimation episode. Such adaptation appears to be facilitated by resorting to gluconeogenic and glycogenolytic pathways for energy mobilization and induction of HSPs.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.236-244
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• 2006

This study was designed to determine the effects of daidzein (DE) on hepatic lipid metabolism in chicks fed with low protein (LP) diet based on casein. In experiment 1, the male chicks were fed with one of the three levels of dietary protein containing 10.95%, 21.9% and 43.8% protein content for 2 days. In experiment 2, the chicks were fed one of the three levels of protein with or without DE at 1,000 mg/kg diet for 2 days. Experiment 3 was conducted to compare DE (LP+DE) with estradiol (LP+E2) in chicks fed with LP diet for 7 days. Plasma lipid profiles, hepatic lipid profiles, activities of hepatic malic enzyme and isocitrate dehydrogenase (ICDH) were measured. Transcriptions of hepatic fatty acid synthase, apolipoprotein-B (APO-B), and fructose bisphosphatase mRNA were measured by RT-PCR. Increasing dietary protein levels markedly decreased the concentrations of plasma triglycerides, hepatic total lipids, hepatic TG, and the mRNA transcriptions while the increased dietary protein levels increased hepatic ICDH activities in experiment 1. In experiment 2, the effects of dietary protein levels on blood and hepatic lipid content were more prominent than those of the additional DE. Interestingly, plasma TG levels were affected by DE supplementation (p<0.05). In experiment 3, DE inhibited APO-B mRNA expressions and stimulated the accumulation of lipid in the liver through mechanisms different from E2. In this study, we demonstrate that DE has beneficial effects on blood lipid profiles, but that it inhibits APO-B mRNA transcription and aggravates the fatty liver induced by LP diet in chicks.

• Genomics & Informatics
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• 제18권4호
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• pp.39.1-39.8
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• 2020

Alzheimer's disease (AD) is a chronic, progressive brain disorder that slowly destroys affected individuals' memory and reasoning faculties, and consequently, their ability to perform the simplest tasks. This study investigated the hub genes of AD. Proteins interact with other proteins and non-protein molecules, and these interactions play an important role in understanding protein function. Computational methods are useful for understanding biological problems, in particular, network analyses of protein-protein interactions. Through a protein network analysis, we identified the following top 10 hub genes associated with AD: PTGER3, C3AR1, NPY, ADCY2, CXCL12, CCR5, MTNR1A, CNR2, GRM2, and CXCL8. Through gene enrichment, it was identified that most gene functions could be classified as integral to the plasma membrane, G-protein coupled receptor activity, and cell communication under gene ontology, as well as involvement in signal transduction pathways. Based on the convergent functional genomics ranking, the prioritized genes were NPY, CXCL12, CCR5, and CNR2.

• Animal Bioscience
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• 제35권7호
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• pp.1010-1020
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• 2022

Objective: The experiment was conducted to evaluate the effects of maternal undernutrition during late pregnancy on the expressions of genes involved in growth and development in ovine fetal perirenal brown adipose tissue (BAT). Methods: Eighteen ewes with singleton fetuses were allocated to three groups at day 90 of pregnancy: restricted group 1 (RG1, 0.33 MJ metabolisable energy [ME]/kg body weight [BW]^0.75/d, n = 6), restricted group 2 (RG2, 0.18 MJ ME/kg BW^0.75/d, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME/kg BW^0.75/d, n = 6). The fetuses were removed at day 140 of pregnancy. All data were analyzed by using the analysis of variance procedure. Results: The perirenal fat weight (p = 0.0077) and perirenal fat growth rate (p = 0.0074) were reduced in RG2 compared to CG. In fetal perirenal BAT, the protein level of uncoupling protein 1 (UCP1) (p = 0.0001) was lower in RG1 and RG2 compared with CG and UCP1 mRNA expression (p = 0.0265) was decreased in RG2. The protein level of myogenic factor 5 (Myf5) was also decreased in RG2 (p = 0.0001). In addition, mRNA expressions of CyclinA (p = 0.0109), CyclinB (p = 0.0019), CyclinD (p = 0.0015), cyclin-dependent kinase 1 (CDK1) (p = 0.0001), E2F transcription factor 1 (E2F1) (p = 0.0323), E2F4 (p = 0.0101), and E2F5 (p = 0.0018) were lower in RG1 and RG2. There were decreased protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (p = 0.0043) and mRNA expression of CCAAT/enhancer-binding protein-α (C/EBPα) (p = 0.0307) in RG2 and decreased PPARγ mRNA expression (p = 0.0008) and C/EBPα protein expression (p = 0.0015) in both RG2 and RG1. Furthermore, mRNA expression of bone morphogenetic protein 4 (BMP4) (p = 0.0083) and BMP7 (p = 0.0330) decreased in RG2 and peroxisome proliferator-activated receptor co-activator-1α (PGC-1α) reduced in RG2 and RG1. Conclusion: Our observations support that repression of regulatory factors promoting differentiation and development results in the inhibition of BAT maturation in fetal perirenal fat during late pregnancy with maternal undernutrition.

• Journal of Nutrition and Health
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• 제40권2호
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• pp.105-110
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• 2007

The study was designed to observe antioxidant activities of conjugated linoleic acid (CLA) by determining antioxidant enzyme protein levels [cytochrome P4502 El (CYP2E1), Copper, Zinc-superoxide dismutase (CuZn-SOD), glutathione peroxidase (CSH-Px), glutathione S-transferase (GST)] by Western blot analysis and the levels of ${\alpha}$-tocopherol and 2-thiobarbituric acid reactive substances (TBARS) in the liver of chronically ethanol-treated rats. Sixty Sprague Dawley male rats were divided into 3 groups (Control, EtOH, EtOH+CLA). All rats were fed Lieber-DeCarli liquid diet for 4 weeks by pair-feeding against the EtOH group. The liquid diet was supplemented with 1.77g CLA mixture per kg diet in the EtOH+CLA group. Isocaloric maltose dextrin was added in replace of 50g ethanol (36%kcal) for the Control group. Ethanol ingestion significantly increased the levels of CYP2E1 protein and TBARS, but significantly reduced CuZn-SOD protein level and increased GST protein level. There was no significant effect on the level of GSH-Px protein and ${\alpha}$-tocopherol in the liver by ethanol. CLA supplementation with ethanol significantly increased the levels of CuZn-SOD, GSH-Px and GST and also significantly attenuated TBARS level, whereas there was no significant effect on the levels of CYP2E1 protein and ${\alpha}$-tocopherol by CLA. Overall, the CLA supplemented to ethanol could significantly increase the levels of CuZn-SOD, GSH-Px and GST proteins and reduce the level of TBARS in the liver of chronically ethanol-treated rats.