• Title/Summary/Keyword: Duck Fat

Search Result 101, Processing Time 0.042 seconds

Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts

  • Ziyi, Pan;Guoqing, Du;Guoyu, Li;Dongsheng, Wu;Xingyong, Chen;Zhaoyu, Geng
    • Journal of Animal Science and Technology
    • /
    • v.64 no.6
    • /
    • pp.1199-1214
    • /
    • 2022
  • Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction.

MicroRNA analysis reveals the role of miR-214 in duck adipocyte differentiation

  • Wang, Laidi;Hu, Xiaodan;Wang, Shasha;Yuan, Chunyou;Wang, Zhixiu;Chang, Guobin;Chen, Guohong
    • Animal Bioscience
    • /
    • v.35 no.9
    • /
    • pp.1327-1339
    • /
    • 2022
  • Objective: Fat deposition in poultry is an important factor in production performance and meat quality research. miRNAs also play important roles in regulating adipocyte differentiation process. This study was to investigate the expression patterns of miRNAs in duck adipocytes after differentiation and explore the role of miR-214 in regulating carnitine palmitoyltransferases 2 (CPT2) gene expression during duck adipocyte differentiation. Methods: Successful systems for the isolation, culture, and induction of duck primary fat cells was developed in the experiment. Using Illumina next-generation sequencing, the miRNAs libraries of duck adipocytes were established. miRanda was used to predict differentially expressed (DE) miRNAs and their target genes. The expression patterns of miR-214 and CPT2 during the differentiation were verified by quantitative real-time polymerase chain reaction and western blot. Luciferase reporter assays were used to explore the specific regions of CPT2 targeted by miR-214. We used a miR-214 over-expression strategy in vitro to further investigate its effect on differentiation process and CPT2 gene transcription. Results: There were 481 miRNAs identified in duck adipocytes, included 57 DE miRNA candidates. And the 1,046 targets genes of DE miRNAs were mainly involved in p53 signaling, FoxO signaling, and fatty acid metabolism pathways. miR-214 and CPT2 showed contrasting expression patterns before and after differentiation, and they were selected for further research. The expression of miR-214 was decreased during the first 3 days of duck adipocytes differentiation, and then increased, while the expression of CPT2 increased both in the transcriptional and protein level. The luciferase assay suggested that miR-214 targets the 3'untranslated region of CPT2. Overexpression of miR-214 not only promoted the formation of lipid droplets but also decreased the protein abundance of CPT2. Conclusion: Current study reports the expression profile of miRNAs in duck adipocytes differentiated for 4 days. And miR-214 has been proved to have the regulator potential for fat deposition in duck.

Effect of Duck-meat Intake on Adult Disease Risk Factors in Adult Human Males

  • Lee, Chang-Jin;Lim, Young-Tae;Kang, Sung-Ho;Jung, Ho-Sung;Kim, Oun-Hyun;Sung, Si-Heung;Song, Hyuk
    • Food Science of Animal Resources
    • /
    • v.30 no.6
    • /
    • pp.951-956
    • /
    • 2010
  • This study was conducted to evaluate the effects of duck-meat consumption on adult disease risk factors, including body compositions, hematological variables, and serum metabolic and lipid profiles in adult human males. To obtain results, 20 adult males aged 20 to 25 were subjected to a diet of 600 g/day of duck-meat for 4 wk, after which body composition, hematological variables, and serum metabolic and lipid profiles were investigated to determine if there was a relationship between duck-meat consumption and adult disease risk factors. The results revealed that high amounts of duck-meat intake did not negatively alter body indices such as body weight, fat mass, body mass index (BMI), % body fat or waste-to-hip ratio (WHR). Furthermore, there were statistically insignificant changes in the number of blood cells, although this number did increase significantly following intake of duck-meat. Moreover, general decreases in serum metabolic parameters were observed, but none of these changes were significant with the exception of the concentration of blood urea nitrogen (BUN). The serum concentrations of LDL-cholesterol also showed a statistically significant decrease by 5.86%. Therefore, this study suggests that the ingestion of duck-meat not only significantly increased the RBC count but also decreased BUN and LDL-cholesterol concentrations in adult males.

Studies on the Fatty Acid Composition of Duck Meat (오리고기의 지방산조성(脂肪酸組成)에 관(關)한 연구(硏究))

  • Nam, Hyun-Keun
    • Journal of Nutrition and Health
    • /
    • v.10 no.1
    • /
    • pp.34-37
    • /
    • 1977
  • Quantitative analysis of the fatty acids contained in Duck meat was carried out by the Gas Chromatography with Flame ionization Detector, The general components and chemical constants have been performed with A.O.A.C. methods. The results art summarized as follows : 1. General composition of Duck meat come out to be 64.87% moisture, 19.06% protein, 17.05% fat, and 1.02% ash. 2. It was investigated that extraction of lipids were performed by Soxhlet extractor for 12 hours. Amounts of lipids were extracted 79.57% in ethylether, 70.15% in chloroform, and 72.35% in n-hexane. 3. Chemical constants of lipids in Duck meat were obtained as follows : Saponification number 201.5, Acid number 5.01, Iodine number 50.1 and Carbonyl number 4.5 4. It was investigated that the fatty acid component were quantitatively determined by the gas chromatography : Linolenic acid 1.6%, Linoleic acid 19.9%, Oleic acid 45.9%, Stearic acid 3.1% Palmitic acid 17.2% and Myristic acid 0.12% in leg portion. Linolenic acid 1.7% Linoleic acid 17.2%, Oleic acid 51.2%, Stearic acid 3.3%, Palmitic acid 17.1% and Myristic acid 0.17% in breast portion. 5. Cholesterol of blood, breast and leg portion fat in Duck were obtained as follows : Total cholesterol 200 mg%, 260 mg% , and 400 mg% respectively; cholesterol ester 120mg%, 151 mg%, and 240mg% respectively.

  • PDF

Studies on the Carcass Characteristics of Broiler by Sex, Rearing Type, Season and Feed and Water Deprivation (육계의 성, 사육형태, 계절 및 절식시간에 따른 도체특성에 관한 조사연구)

  • Hong, J.;Lee, S.J.;Kim, S.S.;Chung, S.B.;Lee, K.H;Oh, S.J.
    • Korean Journal of Poultry Science
    • /
    • v.17 no.1
    • /
    • pp.33-44
    • /
    • 1990
  • This studies were conducted to investigate the difference of carcass characteristics between chicken broiler and duck broiler and the carcass characteristics of chicken broiler by sex, rearing type, season and feed and water deprivation with 240 chicken broilers and 20 duck broilers. The results obtained are summerized as follows. 1. The carcass yields were 65.43% in chicken broiler and 66.78% in duck broiler. The abdominal fat pad of chicken broiler was higher than that of duck broiler. 2. There were differences in commercial cuts production percentages between chicken broiler and duck broiler, and the commercial cut of chicken broiler was produced from drumsticks and thighs, breast back, wings and neck in order. 3. Although there was little difference in carcass yields between male and female of broiler, the abdominal fat pad of male broiler was lower than that of female broiler. 4. The carcass yields in cage and floor type were not different, but the abdominal fat pad in cage was slightly higher than that in floor. 5. There was no difference in carcass yields among Spring, Summer, Autumn and Winter, but the abdominal fat pad was highest in Summer and lowest in Winter. 6. As feed and water deprivation time increased by one hour, live weight was decreased by 0.28365% , and the carcass yield was highest after 6 hours depribated feed and water.

  • PDF

Antibacterial and Deodorant Processing to Remove Odor Substances from Duck Down (덕 다운의 악취 냄새물질 제거를 위한 항균소취 가공)

  • Lee, Bum Hoon;Cho, Hang Sung
    • Textile Coloration and Finishing
    • /
    • v.32 no.4
    • /
    • pp.232-238
    • /
    • 2020
  • Duck down is a feather of waterfowl and is used as an accessory for bedding products such as evil, pillow and outdoor sleeping bags due to its soft and bulky characteristics. Down is used mainly for winter outdoor such as padding and jumpers because of its excellent thermal insulation effect. Down wear is known as a product with high added value. Down is processed and sanitized because a large amount of oil and fat, bacteria, dust, insects, and soil are mixed. In particular, if the proper amount of oil and fat is not removed, it may cause odor or bacteria, and since the fishy smell peculiar to down and harmful VOCs (toluene, benzene, etc.) are released, there is a need to suppress the occurrence of bacteria through the provision of antibacterial function. In this study, we investigated the substances that cause the fishy odor of down, and confirmed the effect on the deodorization and antibacterial properties of down according to processing agents and processing conditions in order to impart deodorant and antibacterial properties to down.

Chemical Composition of Special Poultry (Pheasant, Chungdung Wild Duck, and Ogol Fowl) Meatp (특수가금(꿩, 청둥오리, 오골계)육의 영양성분)

  • Kim, Ki Jun;Oh, Hong Rock;Oh, Man Jin
    • Korean Journal of Agricultural Science
    • /
    • v.23 no.1
    • /
    • pp.90-98
    • /
    • 1996
  • In order to obtain the basic information for new food product development from pheasant, wild duck(chhungdung), and Ogol fowl, which are called as special poultry, general components,fatty acid amino acid and minerals composition were analyzed. 1). The general compositions were 71.2-74.1% of water, 23.3-26.4% of protein, 1.0-2.4% fat, and 1.1-1.4% of ash in the pheasant, 73.8-76.4% of water, 18.6-19.6% of protein, 2.7-6.8% of fat, and 0.8-1.6% of ash in the wild duck, and 71.4-72.3% of water, 20.8-22.7% of protein, 1.9-6.3% of fat, and 1.0-1.2% of ash in the Ogol fowl, repectively. 2).In the pheasant and wild duck meat, the composition of unsaturated and saturated fatty acid were 60-64%,36-40%, respectively, and olec, palmitic, linolec, and stearic acid were most abundant in this order. But, in Ogol fowl, 72-76% and 24-28% were belong to the unsaturated fatty acid, respectively. 3). Amino acid contents in its order of relative amount were glutamic acid, alanine, aspatic acid, and glycine for the pheasant, glutamic acid, glycine, alanine, and aspatic acid for the wild duck, and lysine, glutamic acid, and aspatic acid for the Ogol fowl, repectively. 4).The mineral composition in the pheasant and wild duck were potassium, phosphorus, sodium, magnesium, and calcium, but in the Ogol fowl, potassium, sodium, magnesium, phosphorus, calcium were the most abundant in this order, repectively.

  • PDF

Effect of Addition Duck Skin on Quality Characteristics of Duck Meat Sausages (오리껍질의 첨가가 오리고기 소시지의 품질 특성에 미치는 영향)

  • Kang, Geunho;Seong, Pil-Nam;Cho, Soohyun;Moon, Sungsil;Park, Kyoungmi;Kang, Sun Mun;Park, Beom-Young
    • Korean Journal of Poultry Science
    • /
    • v.41 no.1
    • /
    • pp.45-52
    • /
    • 2014
  • This study was conducted to investigate the effect of additions of duck skin as pork fat replacer on the quality characteristics of duck meat sausage during refrigerated storage. The sausage made with only duck meat was severed as the control. Three treatments of sausages were produced with addition levels (T1, 1%; T2, 2% and T3, 3%) of duck skin. Our results showed that the addition of duck skin significantly (p<0.05) decreased the moisture content whereas significantly (p< 0.05) increased the fat content in the sausages. The sausages with duck skin had significantly (p<0.05) higher pH values until 4 weeks of refrigerated storages when compared to the control. CIE $L^*$ value was significantly (p<0.05) higher in T1 and T2 than those of other treatments after 7 days of refrigerated storage. Similarly the sausages with duck skin had significantly (p<0.05) higher CIE $a^*$ values as compared with the control after 4 weeks of refrigerated storage. The sausages with 1% duck skin processed the significantly (p<0.05) highest hardness, springiness, gumminess and chewiness values at all storage days. No differences were found between the control and treatment for color, flavor, taste, texture and overall acceptability. Based on the results obtained in the present study it is suggested that duck skin to levels of 1~2% can be used as fat replacer for manufacture of duck meat sausage with a positive effect on quality and texture characteristics. However, further research in necessary to improve taste and palatability of the products.

A Study on the Washing Effect Biodegradation of Natural Fat Soap - On Based Biodegradation - (계면활성제 혼합 천연지방산유지 고형 비누의 세척성 및 생분해성 연구 - 생분해성을 중심으로 -)

  • Lee, Bong-Yeon;Ryu, Duck-Hwan;Lee, Tae-Gwan
    • Fashion & Textile Research Journal
    • /
    • v.5 no.3
    • /
    • pp.289-294
    • /
    • 2003
  • The purpose of the study was to get the valuable data for developing the new natural fat soaps which have an excellent biodegradation performance. Thus, natural fat soaps mixed with the two types of detergents (AOS and LAS) on the various concentrations were made and the biodegradation of the samples were analysed by Dissolved Oxygen method using active sludge. Also, the results were compared with the commercial synthetic detergents and market soaps. The results from the study were the followings: 1. The plant fat soap and the wasted oil soap with the concentration of 5 mg/l and 15 mg/l had an excellent biodegradation rather than animal fat soap. 2. There was little difference among samples with the concentration of 5 mg/l, but there was much difference among them with the concentration of 15 mg/l. 3. The periods for consuming oxygen of wasted oil soap mixed AOS and LAS was the fastest.

Nutritional Characteristics and Quality Changes of Duck By-products During Frozen Storage at -20℃ (오리 부산물 종류별 영양학적 특성 및 냉동저장 (-20℃)에 따른 품질변화)

  • Ku, Su Kyung;Hwang, Soon Hoe;Lim, Sang Dong;Lee, Kang Hyun;Kim, Young Boong
    • Food Science of Animal Resources
    • /
    • v.33 no.1
    • /
    • pp.109-118
    • /
    • 2013
  • The objective of this study is to increase the utilization of duck by-products. The nutritional characteristics of four kinds of duck by-products (liver, heart, gizzard, and feet) were determined. The quality changes of four kinds of duck by-products were evaluated during frozen storage at $-20^{\circ}C$. The crude protein and cholesterol contents showed the highest level in liver while the crude fat content was determined to be the highest content in feet at 13.90%, and lowest in gizzard at 0.57%. Duck by-products contained USFA in the range of 48.69-77.66%, and the highest level in feet (p<0.05). During storage of duck by-products at $-20^{\circ}C$, the pH of duck by-products was in the range of 6.24-7.15, and there were no significant differences during the 4 mon storage period at $-20^{\circ}C$. Microbial counts of duck by-products except the gizzard were decreased significantly as storage time elapsed. In the sensory evaluation, overall acceptability of by-products (liver, heart, gizzard and feet) showed a tendency of decreasing value through storage, because off-flavor was increased with increased storage. Considering the combined results, one can conclude that duck by-products provide a good source of protein, and it was judged that the use of raw meat would be most appropriate within 3 mon of frozen storage.