• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.154-162
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• 2012

This study was conducted to evaluate agronomic characteristics such as growth, quality, and yields of StMyb1R-1 transgenic potato and also to obtain the basic data for establishing assessment guidelines of transgenic potato. Three transgenic lines (Myb 1, Myb 2, and Myb 8) were cultivated under conventional irrigation, drought condition, and severe drought condition and were analyzed by comparing with wild type, non-transgenic cv. Superior. Myb 2 showed a different flower color from wild type and Myb 1 had much bigger secondary leaflets than wild type. Myb 1 and Myb 2 showed higher $P_2O_5$ content in both top and root zone and longer shaped tubers than wild type. In yield factors, transgenic lines had more tubers than wild type, however their yield decreases were severe because of the poor enlargement of tuber under water deficit condition. This tendency was noticeable in Myb 1 and Myb 2. In TR ratio, chlorophyll content, dry matter rate, and relative water content, there were no big differences between transgenic lines and wild type. Meanwhile, in phenotype, growth, quality, and yield factors, substantial equivalent was confirmed between Myb 8 and wild type. Then, Myb 8 showed the highest marketable tuber yield under conventional irrigation, while showed lower level than wild type under water deficit. Judged by this result, the enhancing droughttolerance by StMyb1R-1 gene might actually not mean the enhancement of photosynthesis or starch accumulation in tuber and, furthermore, not the yield improvement. More detailed research will be required to accurately understand the relationship between StMyb1R-1 and yield factors.

• Journal of Animal Science and Technology
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• v.62 no.1
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• pp.21-30
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• 2020

This study was conducted to evaluate effects of dietary protease (PR) on growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 75 weaned pigs [7.06 ± 0.18 kg of average body weight (BW); 28 day old] were randomly allotted to 3 dietary treatments in a randomized complete block design (blocks = BW and sex): a diet based on corn and soybean meal to meet the requirement of crude protein (CP) as a positive control (PC; CP = 24.49%), a low protein diet as a negative control (NC; CP = 22.51%), and NC + 0.02% PR. The PR used in this study was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. Pigs were fed the dietary treatments for 6 weeks and the diets containing 0.2% chromic oxide for the last week of this study. Blood, feces, ileal digesta, and ileum samples were collected from randomly selected two pigs in each pen on respective time points. Measurements were growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter (DM), CP, and energy, frequency of diarrhea, packed cell volume (PCV), and ileal morphology of weaned pigs. Pigs fed PC and PR had higher (p < 0.05) final BW, average daily gain (ADG), and gain to feed ratio (G:F) during overall experimental period than those fed NC. Pigs fed PC and PR had higher (p < 0.05) AID or ATTD of DM, CP, or energy than those fed NC. Moreover, pigs fed PR had higher ratio between villus height and crypt depth (p < 0.05) and number of goblet cells (p < 0.05) than those fed NC. Addition of PR decreased (p < 0.05) frequency of diarrhea for the first two weeks after weaning compared with PC and NC. In addition, pigs fed PR had lower (p < 0.05) PCV on d 14 after weaning than those fed PC and NC. In conclusion, addition of PR in nursery diets with a low protein level significantly improved growth performance, nutrient digestibility, and intestinal morphology of weaned pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.950-956
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• 2017

Objective: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Methods: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control. Results: After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The $NH_3-N$ content decreased significantly in inoculant-treated silage (p<0.05) and the $NH_3-N$ content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and in vitro DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05). Conclusion: LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, $NH_3-N$, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.

• Food Science and Preservation
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• v.18 no.4
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• pp.575-583
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• 2011

This study was conducted to investigate the major chemical components of dried baked garlic powder. The proximate compositions of baked garlic powder as a dry-matter basis were 5.30% moisture, 36.89% crude protein, 12.60% crude fat, 4.36% crude ash, 2.88% dietary tiber, and 37.97% carbohydrate, respectively. The major free sugars were identified as fructose and galactose. In the analysis of the total amino acids, 18 kinds of components were isolated from baked garlic powder. The essential amino acid contained in baked garlic powder accounted for 36.60% of total amino acids, while the non-essential amino acids accounted for 63.40%. The major unsaturated fatty acids of total lipids were linoleic acid. The ratio of polyunsaturated fatty acids to saturated fatty acids (P/S ratio) was 5.77. Oxalic acid was the major organic acids. The contents of vitamin A, C, and E were 0.064, 1.452, and 0.037 mg%, respectively. The mineral contents of baked garlic powder were greater, in order of K>Mg>Ca>Na>Zn>Fe. The total polyphenol, flavonoids and thiosulfates contents of baked-garlic ethanol extract were $2.85{\pm}0.05$ mg/100 g, $0.97{\pm}0.04$ mg/100 g and $0.61{\pm}0.02$ OD/g, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.4
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• pp.389-394
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• 2011

This experiment was carried out to find effective weed control method of henbit deadnettle ($Lamium$ $amplexicaule$ L.) on cultivating field of Italian ryegrass ($Lolium$ $multiflorum$ Lam) using herbicide in Grassland and Forages Division, National Institute of Animal Science, RDA, Cheonan from 2008 to 2009. When Onehof was treated two times of spray, weed control ratio was most high as 90%, but this method can not be used because of much damage to Italian ryegrass. When MCPP was treated two times of spray, weed control ratio was high as 82%, and damage to Italian ryegrass was very insignificant. Especially, when the spray of MCPP was treated two times, dry matter yield of Italian ryegrass was 11,427 kg/ha, but that of non treatment was 1,658 kg/ha. That is to say, forage harvest was impossible in non treatment field. According to these results, to control henbit deadnettle on cultivating field of Italian ryegrass using herbicide, you need to treat with two times of spray of MCPP in mid-March. If you do, you can get regular harvest as much as 11,427 kg/ha.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.2
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• pp.134-144
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• 2001

This study was conducted to investigate physicochemical and microbiological properties during composting process when pig manure was composted with some bulking agents(Saw dust, Rice hull, Crushed wood). The pig manure consisted of 2.95% total nitrogen, 4.55% $P_2O_5$, 2.07% $K_2O$, 81.2% organic matter and 14.0 C/N, dry base. The inorganic content of bulking agents were similar one another, and C/N ratio was Saw dust 392. Rice hull 91.5, Crushed wood 266. The temperature of Saw dust composting slowly increased at initial stage, whereas one of Rice hull and Crushed wood rapidly increased and stabilized similarly to outdoor temperature after about 2 month. The pH of compost increased during initial 1 month after decreasing, but thereafter decreased rapidly. The C/N ratio rapidly decreased at initial stage, and it slowly decreased after 1 month. Total nitrogen somewhat increased according to composting process. Ammonium-nitrogen increased until 60th day, and thereafter it was rapidly inclined to decrease. The population of aerobic bacteria, Bacillus spp., actinomycetes and fungi increased up to > $10^{10}cfu\;g^{-1}$ fresh weight, > $10^7$, > $10^8$ and > $10^6$, respectively in Rice hull and Crushed wood composting, and more than ones in Saw dust composting, and specially actinomycetes. The persuasive Bacillus spp. isolated from composting process were B. lentimorbus, B. licheniformis, B. pumilus, B. megaterium, etc.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.415-422
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• 2010

Two leaf lettuces, chicory, endive, edible chrysanthemum, and pak-choi were hydroponically cultured under root restricted conditions in a deep flow technique system and their growth and nutritional values were investigated. Cylindrical plastic tubes with 100 mm height and diameters of 20, 25 or 30 mm were used for root restriction. Growth of all species was retarded by 25-95% as the roots zone was restricted. Pak-choi and edible chrysanthemum showed greater reduction in growth as compared with chicory and endive. Percent dry matter, C/N ratio, and contents of total ascorbic acid and total anthocyanin increased in the root restricted treatments. Contents of P and K decreased in the root restricted treatments, while contents of Mg, Fe, Mn, and Zn were not consistent among the tested species. Optimized root volumes to improve the nutritional values and to reduce the growth retardation varied depending on species of leafy vegetables. Tubes of ${\Phi}25mm{\times}100mm$ and ${\Phi}30mm{\times}100mm$ were suitable for chicory, endive and lettuce, and edible chrysanthemum and pak-choi, respectively. Results indicate that nutritional values of hydroponically-cultured leafy vegetables can be improved by root restriction.

• Korean Journal of Organic Agriculture
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• v.28 no.4
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• pp.615-626
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• 2020

The objective of present experiment was to examine the impact of different organic nitrogen (N) fertilizer application rates and different harvesting periods on the forage yield and feed value of kenaf (Hibiscus cannabinus L.). This study was carried out from May to September 2019, the amount of 80 kg of kenaf seed/ha were applied with different rates of nitrogen fertilizer. The plants were sampled at 10 days intervals (100, 110, 120, and 130 days after seeding) from different harvesting dates. In the organic fertilizer treatments, the highest dry matter (DM) yield was observed in the application rate at 250 kg of N/ha. Crude protein (CP) content in leaves was similar between the organic fertilizer rates at 200 and 250 kg of N/ha and were higher compared with other fertilizer treatments. The highest CP content in the stem was 4.3% in the organic fertilizer application rates st 250 kg of N/ha. Neutral detergent fiber (NDF) in leaves showed no significant difference between the chemical fertilizer rates at 200 and 250 kg of N/ha, and the organic fertilizer rates at 150 and 250 kg of N/ha. In addition, DM yield of kenaf was highest in the harvest of 100 days after seeding, and tended to decreased significantly with increase of harvesting periods (p<0.05). As the growth progressed, the plant height and stem ratio increased but the leaves ratio decreased significantly (p<0.05) and the highest was found at 110 days after seeding. The highest CP, Acid detergent fiber (ADF), NDF and total digestible nutrient (TDN) contents in leaves were 13.9, 25.4, 40.5 and 71.1%, respectively that were affected by different harvesting periods. Also, the ADF and NDF in stem increased significantly with increase of kenaf maturity (p<0.05). In conclusion, the optimal organic fertilizer application rates and the proper harvesting periods for the forage yield and quality of kenaf were at 200 to 250 kg of N/ha, and 100 to 110 days after seeding, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.9
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• pp.1381-1388
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• 2019

Objective: The aims of this study were to reveal the magnitude of the differences in protein structures at a cellular level as well as protein utilization and availability among soybean meal (SBM), canola meal (CM), and rapeseed meal (RSM) as feedstocks in China. Methods: Experiments were designed to compare the three different types of feedstocks in terms of: i) protein chemical profiles; ii) protein fractions partitioned according to Cornell Net Carbohydrate and Protein System; iii) protein molecular structures and protein second structures; iv) special protein compounds-amino acid (AA); v) total digestible protein and energy values; vi) in situ rumen protein degradability and intestinal digestibility. The protein second structures were measured using FT/IR molecular spectroscopy technique. A summary chemical approach in National Research Council (NRC) model was applied to analyze truly digestible protein. Results: The results showed significant differences in both protein nutritional profiles and protein structure parameters in terms of ${\alpha}-helix$, ${\beta}-sheet$ spectral intensity and their ratio, and amide I, amide II spectral intensity and their ratio among SBM, CM, and RSM. SBM had higher crude protein (CP) and AA content than CM and RSM. For dry matter (DM), SBM, and CM had a higher DM content compared with RSM (p<0.05), whereas no statistical significance was found between SBM and CM (p = 0.28). Effective degradability of CP and DM did not demonstrate significant differences among the three groups (p>0.05). Intestinal digestibility of rumen undegradable protein measured by three-step in vitro method showed that there was significant difference (p = 0.05) among SBM, CM, and RSM, which SBM was the highest and RSM was the lowest with CM in between. NRC modeling results showed that digestible CP content in SBM was significantly higher than that of CM and RSM (p<0.05). Conclusion: This study suggested that SBM and CM contained similar protein value and availability for dairy cattle, while RSM had the lowest protein quality and utilization.

• Korean Journal of Poultry Science
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• v.46 no.2
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• pp.87-94
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• 2019

Huge amount of persimmon peels, which are byproducts usually arising after making dried persimmons from fresh persimmons, are generated in fall, every year. As the dry matter of persimmon peel contains carbohydrates over 80%, it could be a good candidate for feed. In this study, we evaluated the persimmon peel as a feed resource for a hen laying eggs. Persimmon peel has lower ratio of crude protein and fat composition while higher ratio of crude fiber composition compared to a basal laying hen feed. Feeding the persimmon peel by adding to the feed at the level of 2% or 5% did not affect the egg-laying rate from beginning to the peak of laying. In addition, feeding the persimmon peel did not affect the egg quality either, as the pH, Haugh unit, yolk color and shell thickness of the egg were not different from each other. The composition of amino acids and fatty acids were also almost same for each other, except for the linolenic acid which was slightly higher in the persimmon peel feeding groups. In conclusion, addition of persimmon peel to the feed up to 5% might be helpful to increase the income and reduce the waste produced from the agricultural byproduct.