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http://dx.doi.org/10.5713/ajas.17.0287

Development of a new lactic acid bacterial inoculant for fresh rice straw silage  

Kim, Jong Geun (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University)
Ham, Jun Sang (Animal Products Research and Development Division, National Institute of Animal Science)
Li, Yu Wei (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University)
Park, Hyung Soo (Grassland and Forage Division, National Institute of Animal Science)
Huh, Chul-Sung (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University)
Park, Byung-Chul (Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.7, 2017 , pp. 950-956 More about this Journal
Abstract
Objective: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Methods: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control. Results: After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The $NH_3-N$ content decreased significantly in inoculant-treated silage (p<0.05) and the $NH_3-N$ content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and in vitro DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05). Conclusion: LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, $NH_3-N$, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.
Keywords
Rice Straw Silage; Inoculant; Lactic Acid Bacteria; Fermentation; Quality;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 MAFRA. The current situation of forage increase production and supplementation policy. Ministry of Agriculture, Food and Rural Affairs; 2015.
2 Han YW, Anderson AW. The problem of rice straw waste a possible feed through fermentation. Econ Bot 1974;28:338-44.   DOI
3 Ralston AT, Kennick WH, Davidson TP, Rowe KE. Effect of prefinishing treatment upon finishing performance and carcass characteristics of beef cattle. J Anim Sci 1966;25:29-33.   DOI
4 Cai Y. Development of lactic acid bacteria inoculant for whole crop rice silage in Japan. Proceeding of satellite symposium of XIIth AAAP Animal Science Congress 2006; Busan, Korea: Asian-Australas Assoc Anim Prod Soc; 2006. p. 85-9.
5 Filya I, Muck RE, Contreras-Govea FE. Inoculant effects on alfalfa silage: fermentation products ans nutritive value. J Dairy Sci 2007;90: 5108-14.   DOI
6 Weinberg ZG, Muck RE. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol Rev 1996;19: 53-68.
7 AOAC. Official method of analysis. 15th ed. Washington, DC: AOAC International; 1990.
8 Goering HK, Van Soest PJ. Forage fiber analysis. Washington, DC: Agric. Handbook 379, U.S. Gov. Print. Office, 1970.
9 Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crop. J Br Grassl Soc 1963;18:104-11.   DOI
10 Jian L, Yixin S, Cai Y. Improvement of fermentation quality of rice straw silage by application of a bacterial inoculant and glucose. Asian-Australas J Anim Sci 2010;23:901-6.   DOI
11 Zhang Z, Scott S, Lukas W, Webb M. A greedy algorithm for aligning DNA sequences. J Comput Biol 2000;7:203-14.   DOI
12 Muck RE. Factors influencing silage quality and their implications for management. J Dairy Sci 1988;55:454-60.
13 Pavlova S, Kilic A, Kilic C, et al. Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol 2002;92:451-9.   DOI
14 Thomas TA. An automated procedure for the determination of soluble carbohydrates in herbage. J Sci Food Agric 1977;28:639-42.   DOI
15 Chaney AL, Marbach EP. Modified reagent for determination of urea and ammonia. Clin Biochem 1962;8:130.
16 APHA. Standard methods for the examination of dairy products. Washington, DC: American Public Health Association; 1993.
17 SAS Institute Inc. SAS/STAT user guide. Cary, NC: SAS Institute Inc.;2002.
18 Kaarel A, Kask S, Laht T, Paalma T. The effect of temoerature and pH on the growth of lactic acid bacteria: a pH-auxostat study. Int'l J Food Microbiol 2003;85:171-83.   DOI
19 Koc F, Coskuntuna L, Ozduven MJ, Coskuntuna A, Samli HE. The effects of temperature on the silage microbiology and aerobic stability of corn and vetch-grain silages. Acta Agric Scand Sec A Anim Sci 2009;59:239-46.
20 Holland C, Kezar W, Kautz WP, et al. The pioneer forage manual-a nutritional guide. Des Moines, IA: Pioneer Hi-Bred International, Inc.; 1990.
21 McDonald P, Henderson N, Heron S. The biochemistry of silage. 2nd ed., Marlow, UK: Chalcombe Publications; 1991. pp. 6-197.
22 Kung L, Satter LD, Jones BA. Microbial inoculation of low moisture alfalfa silages. J Dairy Sci 1987;70:2069-77.   DOI
23 Whiter AG, Kung L. The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of alfalfa silage. J Dairy Sci 2001;84:2195-202.   DOI
24 Wilkins M. Silage UK. 6th edition. Marlow, UK: Chalcombe Publications;1990.
25 Haigh PM. Chemical composition and energy value of big bale silage in Englang 1984-1991. J Agric Eng Res 1995;60:211-6   DOI
26 Rooke JA, Maya FM, Arnold JA, Amstrong DG. The chemical composition and nutritive value of grass silage prepared with no additive or with the application of additives containing either Lactobacillus plantarum of formic acid. Grass Forage Sci 1988;43:87-95.   DOI
27 Zhang YG, Xin HS, Hue JL. Effects of treating whole-plant or chopped rice straw silage with different levels of lactic acid bacteria on sialge fermentation and nutritive value for lactating Holsteins. Asian-Australas J Anim Sci 2010;23:1601-7.   DOI
28 Keady TWJ, Steen RWJ. Effects of treating loe dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action. Grass Forage Sci 1994;49:438-46.   DOI
29 Aksu T, Baytok E, Bolat D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Rumin Res 2004;55:249-52.   DOI
30 Mayne CS. An evaluation of an inoculant of Lactobacillus plantrum as an additive for grass silage for dairy cattle. Anim Prod 1990;51:1-3.   DOI