• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.20 no.5
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• pp.659-665
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• 2011

Recently, the plastic resin such as PC, ABS are widely used in IT market. Especially, in most cases the keypads mounted on the mobile phone are the dual-injection-plated type. Environmental regulation is based on the quality of injection-molded products and the minimum process steps are required to avoid the plating defects. Various parameters to produce the injection-molded plastic products make it difficult to obtain the desired stability. However, the past experience and the use of CAE analysis make it possible to predict the problems occurred in injection molding process. Especially, the problems of the weld lines such as runner balancing, bending, deformation and forming defects can be solved systematically and minimized by CAE analysis. Through this study, the non-uniform volumetric shrinkage and the difference in temperature distribution induce the deformation and the high value of stress causes the problems such as crack.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.33 no.11
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• pp.425-432
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• 1984

This paper is to propse a new self-excitation method of synchronous generator. Instead of conventional exciter of synchronous generator, the additional winding which is arranged in addition to the armature winding, is used in this generator. The output terminal of the additional winding is connected to a capacitor and to a full wave rectifier in series. In this configuration, one source double excitation which is composed of capacitor-self-excitation by lead urrent and direct current excitation by rectifier, is induced. The result is that` The excetation efficency is improved greatly and output waveform is improved also. In three-phase synchronous generator using the new method of the one source double excitation, voltage element (shunt characteristics) and current element (series characteristics)are compounded in scalar by adapting star-point-open-rectifier system. The result is as following` The effect of load power factor angle on voltage regulation is reduced greatly, compound characteristics is become manifold by controlling capacity of capacitor, and transient response is improved.

• Animal cells and systems
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• v.9 no.3
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• pp.145-152
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• 2005

A retrograde tracer, WGA-apo-HRP-gold, was injected into midline thalamic nuclei and subsequently orexin-A immunostaining was performed on the tuberal region of the hypothalamus in order to investigate orexinergic projections to the midline thalamus. Injection site was targeted within one specific region, i.e., paraventricular, centromedian, rhomboid, reuniens, or intermediodorsal nucleus, but it proved to be either one or a combination of these thalamic nuclei. The distribution of WG/orexin-double-labeled neurons exhibited a general pattern in that the majority of labeled cells were observed within the ventral portion of the lateral hypothalamus as well as the perifornical nucleus (PeF). A small number of double-labeled cells were also observed at the dorsomedial nucleus, the area dorsal to the PeF, dorsal portion of the lateral hypothalamus, and the posterior hypothalamus. These orexin-immunoreactive neurons might have wake-related influences over a variety of functions related with midline thalamic nuclei, which include autonomic control, associative cortical functions, and limbic regulation.

• BMB Reports
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• v.47 no.11
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• pp.619-624
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• 2014

Antisense RNA is a type of noncoding RNA (ncRNA) that binds to complementary mRNA sequences and induces gene repression by inhibiting translation or degrading mRNA. Recently, several small ncRNAs (sRNAs) have been identified in Escherichia coli that act as antisense RNA mainly via base pairing with mRNA. The base pairing predominantly leads to gene repression, and in some cases, gene activation. In the current study, we examined how the location of target sites affects sRNA-mediated gene regulation. An efficient antisense RNA expression system was developed, and the effects of antisense RNAs on various target sites in a model mRNA were examined. The target sites of antisense RNAs suppressing gene expression were identified, not only in the translation initiation region (TIR) of mRNA, but also at the junction between the coding region and 3' untranslated region. Surprisingly, an antisense RNA recognizing the upstream region of TIR enhanced gene expression through increasing mRNA stability.

• Environmental and Resource Economics Review
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• v.10 no.1
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• pp.95-126
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• 2001

Functional values for wastewater treatment and atmospheric regulation in coastal wetland and rice paddy ecosystems are quantified, and an illustration is given on how to integrate biophysical parameters into a valuation framework. This is one of most controversial issues in economic analysis for wetland preservation versus wetland conversion to agricultural use. This paper includes theoretical considerations for estimating functional values of environmental ecosystems, and the integration of biophysical data and replacement cost method employed. Specific physical and geographical characteristics and data on ecosystem functions and services in coastal wetlands and rice paddies are addressed for evaluating their values in economic terms. In particular this paper indicates double counting problems and overestimation in the previous studies, and demonstrates how to avoid them and to maintain the consistency of valuation process involving a least-cost method, thus enables an accurate integration of the coastal wetland ecology and wetland economics. As a result which is far away from the previous studies, the total economic present value of wastewater assimilation by coastal wetland is estimated at 7,484,640 won/ha, and the net present value of positive effect for atmospheric regulation, negative effects for air pollution and water pollution by rice paddy is estimated at -37,934 won/ha, assuming that resources are infinitely long-lived and the annual value and the rate of discount (10%) is constant every year. In conclusion, for further reliability and validity of functional values for natural resources it is very noteworthy that a general equilibrium framework that could directly incorporate the interdependence between ecosystem functions and services would be preferred to the partial equilibrium framework.

• The KSFM Journal of Fluid Machinery
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• v.16 no.1
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• pp.17-23
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• 2013

Recently so much attention has been focused on renewable energy and, since its sources to exploit are already almost saturated in the country, the practical alternative to this situation could be a micro-turbine which uses the low head and low flow. From a point of view of local micro-turbine design capacity and manufacturing technology, the problems such as the accumulation of technical skills, the expansion of related industries, the national policy expansion and the turbine efficiency to improve are still vulnerable and it's true that there are also negative views about the economic feasibility, the technicity and the operation management of the micro-turbine. However, if the improvement can be done in technology of low-head double regulation micro-turbine to generate more outputs and the operation management can be reliably realized, the micro-turbine will be re-evaluated as an appliable source of renewable energy, even the output is small, and by a paradigm shift, it could realize a power generation as an economic and rational system.

• The Transactions of the Korean Institute of Power Electronics
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• v.19 no.3
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• pp.276-282
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• 2014

In a single-phase grid-connected power system consisting of a dc-dc converter and a dc-ac converter, the current drawn from renewable energy sources has a tendency to be pulsated and contains double-fundamental frequency ripple components, which results in several drawback such as a power harvesting loss and a shortening of the energy source's life. This paper presents a new double-fundamental current reduction-scheme with a fast dc-link voltage loop for two-stage dc-dc-ac grid connected systems. In the frequency domain, an adequate control design is performed based on the small-signal transfer function of a two-stage dc-dc-ac converter. To verify the effectiveness of proposed control algorithm, a 1 kW hardware prototype has been built and experimental results are presented.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.186-195
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• 2015

Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.

• Journal of Environmental Policy
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• v.11 no.2
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• pp.17-54
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• 2012

This paper researched market responses for listed companies in several industries affected by the major oil spill accident off the coast of Taean, in December 7, 2007. The Taean accident triggered considerable concerns in people over the possibility of potential future regulation in shipbuilding and petroleum industries. However, the accident also provided an unexpected business opportunity for environmental clean-up industry and shipbuilding industry. The oil spill triggered the acceleration of the enactment of policies that require all new oil tankers to be constructed with double hull, which is interpreted as a good news for shipbuilding industry. Increased public pressure coupled with the prospect of tightened regulation is expected to decrease the market values of firms in the affected business fields. The stock prices of shipbuilding companies dropped after the incident but dramatically surged after the enactment of the policy in January 31, 2008. Our study also found that companies with more extensive prior environmental disclosure had less negative market reactions during the first sixteen days following the accident.

• BMB Reports
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• v.43 no.3
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• pp.164-169
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• 2010

CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence-independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.