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http://dx.doi.org/10.5483/BMBRep.2010.43.3.164

The production and immunostimulatory activity of double-stranded CpG-DNA  

Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University)
Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University)
Rhee, Jae-Won (Center for Medical Science Research, College of Medicine, Hallym University)
Kim, Min-Soo (Department of Microbiology, College of Medicine, Hallym University)
Seok, Hyun-Jeong (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University)
Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University)
Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
Publication Information
BMB Reports / v.43, no.3, 2010 , pp. 164-169 More about this Journal
Abstract
CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence-independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.
Keywords
dsCpG-DNA; HLA-DR; IL-8; Innate immunity; Lipofectin;
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