• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.1
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• pp.121-131
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• 2015

Passive emitter localization is preferred to use a small number of receivers as possible for the efficiency of strategic management in the field of modern electronic warfare support. Accurate emitter localization can be expected when utilizing continuous measurable parameters and a appropriate combination of theirs. For this reason, we compare CRLB (Cramer-Rao lower bound) of two moving platform with various measurable parameters to choose a appropriate combination of parameters for a better localization performance. And we propose the passive emitter localization method based on Levenberg-Marquardt algorithm with combined TDOA/FDOA and DOA to achieve better accuracy of emitter localization which is located on the ground and stationary. In addition, we present a method for determining the initial emitter position for LM algorithm's input to avoid the divergence of estimation and local minimum.

• Atmosphere
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• v.25 no.3
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• pp.543-557
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• 2015

The present study examines an interaction between the eddy and mean meridional circulation (MMC) comparing the results in three reanalysis data sets including ERA-Interim, NCEP2, and JRA-55 during the boreal winter in the Northern Hemisphere. It is noteworthy that the JRA-55 tends to produce stronger MMC compared to those of others, which is mainly due to the weak eddy flux. ERA-Interim represents the ensemble averages of MMC. The MMC-eddy interaction equation was adopted to investigate the scale interaction of the eddy momentum flux (EMF), eddy heat flux (EHF), and diabatic heating (DHT) with MMC. The EMF (EHF) shows a significant correlation coefficient with streamfunction under (above) 200 hPa-level. The perturbation (time mean) part of each eddy is dominant compared to another part in the EMF (EHF). The DHT is strongly interacted with streamfunction in the region between the equator and extra-tropical latitude over whole vertical column. Thus, the dominant term in each significant region modulates interannual variability of MMC. The inverse (proportional) relationship between MMC and pressure (meridional) derivative of the momentum (heat) divergence contributions is well represented in the three reanalysis data sets. The region modulated interannual variability of MMC by both EMF and DHT (EHF) is similar in ERA-Interim and JRA-55 (ERA-Interim and NCEP2). JRA-55 shows a lack of significant region of EHF due to the high resolution, compared to other data sets.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.876-887
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• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• Journal of Microbiology and Biotechnology
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• v.28 no.7
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• pp.1185-1198
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• 2018

The nucleosomal organization of chromatin using histone proteins is a fundamental and ubiquitous feature of eukaryotic nuclei, with the major exception of dinoflagellates. Although a number of recent genomic and transcriptomic analyses have detected numerous histone genes in dinoflagellates, little is known about their expression. Here in, we aimed to investigate the expression pattern of histone genes under nutritional stress, and an attempt was made to detect histone expression at the protein level in Alexandrium pacificum. The presence of histones at the mRNA level was confirmed in this study by the amplification, cloning, and sequencing of 10 different genes. Relative expression profiling of these genes under different growth conditions was determined with real-time PCR and revealed considerable levels of histone transcription in nutritionally stressed cells. We were unable to detect the expression of histones at the protein level even after immunodetection and analysis using mass spectrometry, although a histone-like protein was detected as a major nuclear component. A. pacificum expresses multiple variants of histone, and protein sequences revealed both conservation and divergence with respect to other eukaryotes. We concluded that A. pacificum maintained an active transcription of histone genes within the cell, and enhanced expression of histone genes in nutritional stress strongly suggest that histones have functional significance in dinoflagellates, although expression at the protein level was below our current detection limits, which suggests a limited role of histones in DNA packaging. Finally, the plausible regulation of histone expression at the gene and protein levels in A. pacificum is discussed.

• Archives of design research
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• v.14 no.3
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• pp.77-85
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• 2001

This paper focuses on the idea development as a creative thinking process for design innovation. The process of thinking has the thinking structures of abduction and transformation. After we had studied the design thought, we found a structure of a thinking system, and created a creative thinking model with this. Using job analysis, we examined the duster of design jobs, which form the design process, and verified the thinking model. The findings suggest that our idea development has the creative process not only of divergent thinking and convergent one, but also of transformation in design. In same time, the design thinking shows their pattern of transition from abstract concept to concrete object. Between the design jobs, idea development shows higher difficulty than other jobs - marketing, product planning and follow-up. Combining the D-T-C (Divergent-Transformation-Convergent) thinking with abstract-concrete thinking, we designed a DFD(data flow diagram) for an early model of computer mediated thinking system (CMTS). This has implications for design support.

• Geophysics and Geophysical Exploration
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• v.1 no.3
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• pp.161-169
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• 1998

The Kunsan basin is a pull-apart basin which was formed during Tertiary. The pre-Tertiary section consists of various rock types, such as meta-sediments, igneous rocks, carbonates, clastics, and volcanics. Tertiary sections are the main targets for the petroleum exploration. In order to determine the optimum processing parameters of the basin, about 12 kinds of test processings were performed. The first main steps for the quality control is to determine the noisy or bad traces by examining the near trace section and shot gathers. The true amplitude recovery was applied to account for the amplitude losses due to spherical divergence and inelastic attenuation. Source designature and predictive deconvolution test were conducted to determine the optimum wavelet parameters and to remove the multiples. Velocity analysis was performed at 1km intervals. The optimum mute function was picked by locating the range of offsets which gives the best stacking response for any particular reflections. Post-stack deconvolution was tested to see if the quality of stacked data improved. The stacked data was migrated using a finite difference algorithm. The migration velocity was obtained from the stacking velocities using the time varying percentages. The AGC sections were provided for the structural interpretation. The RAP sections were used for DHI analysis and for the detection of volcanics.

• Bulletin of the Korean Chemical Society
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• v.34 no.4
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• pp.1153-1159
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• 2013

A novel solid-contact indium(III)-selective sensor based on bis-(1H-benzimidazole-5-methoxy-2-[(4-methoxy-3, 5-dimethyl-1-pyridinyl) 2-methyl]) thiosulfinate, known as an omeprazole dimer (OD) and a neutral ionophore, was constructed, and its performance characteristics were evaluated. The sensor was prepared by applying a membrane cocktail containing the ionophore to a graphite rod pre-coated with polyethylene dioxythiophene (PEDOT) conducting polymer as the ion-to-electron transducer. The membrane contained 3.6% OD, 2.3% oleic acid (OA) and 62% dioctyl phthalate (DOP) as the solvent mediator in PVC and produced a good potentiometric response to indium(III) ions with a Nernstian slope of 19.09 mV/decade. The constructed sensor possessed a linear concentration range from $3{\times}10^{-7}$ to $1{\times}10^{-2}$ M and a lower detection limit (LDL) of $1{\times}10^{-7}$ M indium(III) over a pH range of 4.0-7.0. It also displayed a fast response time and good selectivity for indium(III) over several other ions. The sensor can be used for longer than three months without any considerable divergence in potential. The sensor was utilized for direct and flow injection potentiometric (FIP) determination of indium(III) in alloys. The parameters that control the flow injection method were optimized. Indium(III) was quantitatively recovered, and the results agreed with those obtained using atomic absorption spectrophotometry, as confirmed by the f and t values. The sensor was also utilized as an indicator electrode for the potentiometric titration of fluoride in the presence of chloride, bromide, iodide and thiocyanate ions using indium(III) nitrate as the titrant.

• Molecules and Cells
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• v.26 no.1
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• pp.53-60
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• 2008

We characterized the human endogenous retrovirus (HERV-W) family in humans and primates. In silico expression data indicated that 22 complete HERV-W families from human chromosomes 1-3, 5-8, 10-12, 15, 19, and X are randomly expressed in various tissues. Quantitative real-time RT-PCR analysis of the HERV-W env gene derived from human chromosome 7q21.2 indicated predominant expression in the human placenta. Several copies of repeat sequences (SINE, LINE, LTR, simple repeat) were detected within the complete or processed pseudo HERV-W of the human, chimpanzee, and rhesus monkey. Compared to other regions (5'LTR, Gag, Gag-Pol, Env, 3'LTR), the repeat family has been mainly integrated into the region spanning the 5'LTRs of Gag (1398 bp) and Pol (3242 bp). FISH detected the HERV-W probe (fosWE1) derived from a gorilla fosmid library in the metaphase chromosomes of all primates (five hominoids, three Old World monkeys, two New World monkeys, and one prosimian), but not in Tupaia. This finding was supported by molecular clock and phylogeny data using the divergence values of the complete HERV-W LTR elements. The data suggested that the HERV-W family was integrated into the primate genome approximately 63 million years (Myr) ago, and evolved independently during the course of primate radiation.

• Genomics & Informatics
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• v.3 no.2
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• pp.61-65
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• 2005

Comparing 231 genes on chimpanzee chromosome 22 with their orthologous on human chromosome 21, we have found that 15 orthologs have indels within their coding sequences. It was rather surprising that significant number of genes have changed by indel, despite the shorter time since their divergence and led us hypothesize that indels and structural changes may represent one of the major mechanism of proteome evolution in the higher primates. Human T-complex protein 10 like (TCP 10L) is a representative having indel within its coding sequence. Gene structure of human TCP10L compared with chimpanzee TCP10L gene showed 16 base pair difference in genomic DNA. As a result of the indel, frame shift mutation occurs in coding sequence (CDS) and human TCP10L express longer polypeptide of 21 amino acid residues than that of chimpanzee. Our prediction found that the indel may affect to dramatic change of secondary protein structure between human and chimpanzee TCP10L. Especially, the structural changes in the C-terminal region of TCP10L protein may affect on the interacting potential to other proteins rather than DNA binding function of the protein. Through these changes, TCP10L might influence gene expression profiles in liver and testis and subsequently influence the physiological changes required in primate evolution.

• Geophysics and Geophysical Exploration
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• v.10 no.2
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• pp.154-160
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• 2007

This paper compares the characteristics of three different algorithms for three-dimensional (3D) magnetotelluric (MT) modeling. These methods are developed by Mackie et al. (1994), Sasaki (1999) and Nam et al. (2007). The first and second methods are based on the finite difference method (FDM), while the last one the finite-element method (FEM). MT responses, apparent resistivities and phases, for a COMMEMI 3D-2 model show a good agreement with integral equation solutions and only minor discrepancies are found over the anomalous bodies in the 3D model. The computation time of the two methods based on FDM is short and the static divergence correction contributes to speed up. The FEM modeling scheme is accurate but slow.