• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1498-1504
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• 2005

Competitive direct ELISA was developed to detect gentamicin residues. Mice immunized with gentamicin-keyhole limpet hemocyanin (KLH) conjugate developed good antiserum titers, which gradually increased with booster injections, indicating immunization was successfully processed. Monoclonal antibody against gentamicin was prepared using hybridoma cells cloned by limit dilution of fused cells. IgG was purified from ascites fluid of hybridoma cell-injected mice through ammonium sulfate precipitation and Sephadex G-25 gel filtration. After the gel filtration, fractions of high antibody titer were further purified through affinity chromatography on protein A/G column. Monoclonal antibody against gentamicin was confirmed as IgG1, which has kappa light chain. Cross-reactivities ($CR_{50}$) of gentamicin monoclonal antibody to other aminoglycosides (kanamycin, neomycin, and streptomycin) were less than 0.005%, indicating the monoclonal antibody was highly specific for gentamicin. Standard curve constructed through competitive direct ELISA showed measurement range (from 80 to 20% of B/$B_0$ ratio) of gentamicin was between 1 and 40 ng/ml, and 50% of B/$B_0$ ratio was about 4 ng/ml. The gentamicin concentration rapidly increased to 1,300 ng/ml after the intramuscular administration up to 2 h, then sharply decreased to less than 300 ng/ml after 4 h of withdrawal, during which the elimination half-life ($t_{1/2}$) of gentamicin in the rabbit plasma was estimated to be 1.8 h. Competitive direct ELISA method developed in this study using the prepared monoclonal antibody is highly sensitive for gentamicin, and could be useful for detecting gentamicin residues in plasma of live animals.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.225-232
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• 1992

In order to develop an enzyme-linked immunosorbent assay(ELISA) for detecting aflatoxin $B_1(AFB_1)$, we produced and purified antibodies, thereafter established and evaluated methods of direct and indirect competitive ELISA. Anti-AFB, antisera, produced by immunizing rabbits with $AFB_1$-1-(0-carboxymethy1)oxime-bovine serum albumin conjugate ($AFB_1$-BSA), were removed of anti-BSA antibodies by quantitative precipitation reaction and further purified by ammonium sulfate precipitation and DEAE-Sephadex A-50 ion exchange chromatography. Purified IgG fractions were used as anti-$AFB_1$ antibodies. The antibodies, whose titer was deterrnined extremely high above $2 \times 10^6$, showed low cross-reactivity of 3~34% against $AFB_1$ analogues such as G2, B2, and GI. From the standard curves of direct and indirect competitive ELISA for AFBI, the detection ranges were found 0.2~20 and 1~10, 000 ng/ml(ppb) respectively. In their sensitivity, stability, simplicity, and rapidity, the direct method was more suitable than the indirect method for practical use.

• Journal of Veterinary Clinics
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• v.29 no.1
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• pp.18-22
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• 2012

This study was performed to evaluate the sensitivity of conventional film-screen radiography (CFSR) and direct digital radiography (DDR) for detection of various amounts of free peritoneal fluid. Ten adult male healthy beagles were used in this study. Radiographic examinations were performed in the right lateral and ventrodorsal positions. Fluid was injected in increments of 2.0 ml/kg of body weight up to 20.0 ml/kg of body weight. The images of CFSR and DDR were evaluated by two veterinary radiologists for evidence of abdominal fluid without knowledge of injected fluid volume. Data were evaluated by using the receiver operation curve (ROC) analysis and the area under the curve (AUC). There was no significant difference in detection of peritoneal fluid between DDR and CFSR in the ROC analysis. The accuracy of CFSR (0.805) was relatively higher than that of DDR (0.733), based on the ROC analysis and AUC. AUC of CFSR was higher in most injection doses. These results suggest that CFSR is more accurate than DDR for the detecting peritoneal fluid. Therefore, for situation in which digital radiographs are equivocal or small amount of fluid is suspected, other imaging modalities, such as ultrasonography would be helpful for determining the presence of fluids.

• Journal of Life Science
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• v.10 no.6
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• pp.630-639
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• 2000

The gastric pathogen Helicobacter pylori(H. pylori) establishes long-term chronic infection that can lead to atrophic gastritis, intestinal metaplasia, and gastric cancer. H. pylori, which express cytotoxic genes is now recohnized as a cause of peptic ulcer and is also a major risk factor for the development of gastric adenocarcinoma. We performed this study 1) to assess the detection rate of H. pylori according to direct investigation of bacteria of gastric biopsy specimen and two serologic tests of GAP test and Helico blot 2.0 system in the symptomatic and non-symptomatic group 2) to evaluate and compare the efficacy of two serologic tests of GAP test and Helico blot 2.0 system for the diagnosis of H. pylori infection. Forty-nine patients were positive for H pylori infection based on direct investigation of bacteria by histology. The detection rates of H. pylori infection based on direct investigation of bacteria by histology. The detection rates of H. phlori were significantly lower in gastric cancer than in other gastroduodenal disease(p<0.05). The concordance of two serologic tests of GAP test and Helico blot 2.0 system is poor. There was no statistically significant difference between the expression rate of CagA and VacA in the symptomatic and non-symptomatic group. Although Helico blot 2.0 system may not displace GAP test, it was a very sensitive serologic test for the diagnosis of H. pylori infection and it was used to detect IgG antibodies to H. pylori-specific antigens, including CagA, VacA and the various urease subunit. Our data suggest that further investigation is needed to determine whether or not the serologic expression of cytotoxic gene may be clinical usefulness of diagnostic methods in the gastroduodenal disease.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2007.04a
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• pp.289-293
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• 2007

Because the current numerical value map and update system in use do not reflect rapidly enough the transformation of topography, their usage are gradually in decrease. Therefore, there is an increasing demand for reception update system of numerical value map which can guarantee the accuracy and up-to-dateness of data. While rapid detection of topographical transformation is essential for rapid updating, the existing research based on remote investigation or direct measurement was difficult to apply efficiently in terms of cost and accuracy. Thereupon, this research aims to present efficient methods of detecting topographical transformations for a frequent real time updating of numerical value map using the topographical transformation and related data inputted in real time into the administrative information system.

• Proceedings of the IEEK Conference
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• 2000.06a
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• pp.252-255
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• 2000

In this paper, a fast pseudo-noise (PN) code acquisition with novel adaptive architecture is presented in direct-sequence spread- spectrum (DS-SS) systems. Since an existing acquisition system has a fixed correlation tap size and threshold value, this system cannot adapt to various mobile communication environments and results in a low detection probability or a high false alarm rate and long acquisition time. Therefore, if a correlation tap size and a threshold value can be controlled adaptively according to received signals, problems of ail existing system will be solved. The system parameter varies adaptively by using constant false alarm rate (CFAR) algorithm well known in a field of detection and proposed signal-to-noise ratio (SNR) measurement system. By deriving formulas of the proposed system, the performance is analyzed.

• The Journal of the Acoustical Society of Korea
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• v.28 no.3E
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• pp.79-87
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• 2009

This paper reviews several multi-source localization methods which estimate ITDs based on zero crossings (ZCs). Employing signal-to-noise ratio (SNR) estimation from ITD variances, these ZC-based source localization methods are more robust to diffuse noise than the cross-correlation (CC)-based one with less computational complexity. In order to take reverberant environments into account, two approaches detect intervals which dominantly contain direct-path components from sources to sensors because they may effectively provide reliable ITDDs corresponding to source directions. One accomplishes the detection by comparing the original and cepstral-prefiltering-processed envelopes, and the other searches sudden increase of acoustic energy by considering typical characteristics of acoustic reverberation. Experiments for comparison of these methods demonstrate that the approach with energy-based detection efficiently achieves multi-source localization in reverberant environments.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.11
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• pp.2941-2956
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• 2013

In this paper, we propose an effective fragile image watermarking scheme for tampering detection and content recovery. Cover image is divided into a series of non-overlapping blocks and a block mapping relationship is constructed by the secret key. Several DCT coefficients with direct current and lower frequencies of the MSBs for each block are used to generate the reference bits, and different coefficients are assigned with different bit numbers for representation according to their importance. To enhance recovery performance, authentication bits are generated by the MSBs and the reference bits, respectively. After LSB substitution hiding, the embedded watermark bits in each block consist of the information of itself and its mapping blocks. On the receiver side, all blocks with tampered MSBs can be detected and recovered using the valid extracted reference bits. Experimental results demonstrate the effectiveness of the proposed scheme.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.1 s.94
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• pp.81-86
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• 2005

In this work, we propose a new ultrasonic damage inspection method in plate structures. The proposed method employs an OPMT(orientation-adjustable patch-type magnetostrictive transducer) in order to make the ultrasonic waves directed to a specific target point. For experiments, virtual grid points were set up at every 50 mm in an aluminum plate and two OPMTs were used for inspection. If there exists a crack in a plate, the reflected Lamb wave from the crack is measured in addition to the direct waves from the transmitting transducer to the receiving transducer.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.284-288
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• 2005

This paper proposes the direct detection method of phase angle for the power source, which is based on the PLL method. The proposed method using a bidirectional photo-coupler is used to directly detect the zero crossing of phase voltage and calculate the angular frequency in the controller based on a M/T algorithm. Through the method, the additional installation space in the traditional method using a potential transformer can be minimized and it can be easy to design. The paper presents straightforward schematic circuits, design and experimental results.