• 제목/요약/키워드: Different extracting conditions

검색결과 62건 처리시간 0.023초

추출조건에 따른 인삼농축액 중의 잔류용매 및 무기물 함량 (Residual Solvents and Mineral Contents in Ginseng Extracts with Different Extracting Conditions)

  • 이선화;김우성;김용무;홍영표;안영순;박홍재;김옥희
    • 한국환경과학회지
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    • 제16권1호
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    • pp.39-44
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    • 2007
  • This study was conducted to investigate the contents of residual solvents and mineral components(11 kinds) in ginseng extracts with different extracting conditions(5 types) and commercial ginseng extract products(domestic, imported). Fine root was extracted with solution having various ethanol concentration after hexane treatment. Among 5 type extracts, residual solvent(hexane) was detected ginseng extracts treated ethanol mixed with hexane. But extracts that dried after soaked in hexane wasn't detected hexane. Mineral components(Al, Mn, Fe, Cu and Zn) were detected in fine root and 5 types of extracts. The contents of mineral components between fine root and extracts with various extracting conditions were similar, however, extracts that dried after soaked in hexane showed the lower amount in Al, Fe, Pb than the others. In comparison with commercial ginseng ex-tract products(domestic, imported), the distribution pattern of mineral was similar but the contents were a little different.

Efficiency for extracting icariin from Epimedium koreanum Nakai by temperature and solvent variations

  • Baek, Hum-Young;Lee, Young-Sang
    • Plant Resources
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    • 제6권3호
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    • pp.221-226
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    • 2003
  • To improve industrial scale extraction method for extraction of icariin from Epimedium koreanum Nakai, the yields under different extracting conditions such as solvent, temperature, duration and solvent to plant material weight ratio were compared. Regarding extracting solution, highest extracts and icariin yield could be achieved when 10% EtOH was used. In case of plant material to extracting solvent ratio, no significant differences could be observed from 1/10 to 1/50, indicating 1/10 was the most efficient. Extracting temperature significantly affected extracts and icariin yields in that 9$0^{\circ}C$ increased the collected extracts and icariin contents up to 29.6% and 0.76%, respectively, compared to 27.2%, 0.33% at 7$0^{\circ}C$. The yield of extracts was less dependent upon extracting temperature compared to icariin yield. Regarding extraction time, 4 hr and 6 hr resulted in high extracts and icariin yield, respectively. We found extracting Epimedium koreanum Nakai in 10 times volume of 10% EtOH for 4 and 6 hr at 9$0^{\circ}C$ seem to be relatively efficient methods for extracts and icariin, respectively.

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약용식물의 부위별 및 추출용매에 따른 효능 연구 (The Study on the Efficacy of Herbal Plant Extracts by the Part and Solvent Extraction)

  • 김경동;김상진
    • 대한화장품학회지
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    • 제33권2호
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    • pp.127-135
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    • 2007
  • 본 연구는 한방화장품의 원료로써 사용이 되는 약용식물들의 부위별 및 추출용매에 따른 추출물의 효능의 차이를 기술하였다. 기존의 단일성분 분석법보다 실제로 성분의 복합체인 추출물에 대하여 항산화와 UV흡수 관련 효능효과 시험법을 적용하였다. 약효를 가지는 약용식물들을 추출할 때 고려해야 할 조건이 있다. 같은 약용식물이라도 채취시기, 채취장소, 채취부위와 같은 채취조건들과 용매의 종류, 추출시간, 추출온도와 같은 추출조건들을 고려하여야 한다. 조건 중에서 추출용매와 채집부위는 효과에 있어서 매우 중요한 요인이다. 약용식물에 대한 관심이 증가하면서 여러 가지의 채취조건과 추출조건을 연구하여 한방원료의 개발에 있어 좀더 다양한 적용이 요청되고 있다. 본 연구에서는 약용과 식용으로 널리 사용되는 식물(Terminalia chebula, Syzygium aromaticum, Paeonia lactiflora, Morus alba, Scutellaria baicalensis)을 선정하여 추출용매와 채집부위를 변경시킴으로써 용매와 부위에 따른 효능의 차이를 확인하였다. 이 결과로 추출시 적당한 조건을 선택한다면 사용되는 약용식물의 양을 줄이거나 좀더 효과적인 결과를 얻을 수 있다.

추출조건에 따른 홍삼추출액의 사포닌 조성 및 이화학적 특성 (Saponin Composition and Physico-Chemical Properties of Korean Red Ginseng Extract as Affected by Extracting Conditions)

  • 이승호;강정일;이상윤
    • 한국식품영양과학회지
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    • 제37권2호
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    • pp.256-260
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    • 2008
  • 홍삼은 건강을 유지하기 위한 약재로 오래전부터 우리나라에서 많이 애용되고 있는 한방소재로서 근래에 와서 홍삼의 유효 성분군이 사포닌으로 밝혀지면서 약리 효능적인 측면에서는 연구가 급진전되었으나, 홍삼의 유용 사포닌 성분을 안정성 높게 회수하기 위한 추출방법 및 홍삼을 추출하는데 있어 이화학적인 특성에 영향을 주는 조건에 대해서는 현재까지 연구된 바가 많지 않아 홍삼 추출조건에 대한 체계적인 연구가 필요한 실정이다. 이에 본 연구에서는 홍삼 추출기를 이용하여 추출조건에 따른 사포닌 성분 및 이화학적 변화에 대해 조사하여 비교분석하였다. 홍삼추출액의 총 사포닌 함량에 있어서는 추출온도와 시간이 증가함에 따라 총 사포닌 함량이 감소하는 경향을 나타내었으며, $75^{\circ}C$, 24시간 추출조건에서 0.774 mg/g으로 가장 높은 함량을 나타내었다. 개별 ginsenoside 함량에서도 대부분 추출온도와 시간이 증가될수록 함량이 감소하였지만, $Rg_3$의 경우에는 일정범위내에서의 추출조건에서는 함량이 오히려 증가하는 경향을 나타내었다. 이는 홍삼 추출 시 가열 과정에서 일부 ginsenoside가 $Rg_3$로 구조 전환되면서 그 함량이 증가한 것으로 추정된다. 또한 추출조건별 홍삼추출액의 이화학적 변화에 있어서는 사포닌의 경우와 반대로 추출온도와 시간이 증가될수록 $^{\circ}Brix$와 색차는 증가되는 반면, pH는 감소하는 등 전반적으로 품질 측면에서는 안정화되는 경향을 나타내었으며, 품질적인 특성만을 고려한 적정 추출조건은 $85^{\circ}C$ 내외에서 48시간 추출하는 것이 효율적이라 판단된다. 따라서 이러한 결과를 바탕으로 홍삼 추출액의 품질특성과 사포닌의 안정적인 추출을 위한 적정 추출조건은 ginsenosides의 손실이 최소화되는 $80^{\circ}C$ 내외에서 물리적 성질이 안정화되기 시작하는 48시간 이내로 추출하는 것이 적합한 것으로 사료된다. 이와 같은 결과는 홍삼 추출방법에 있어서 최적의 추출조건에 대한 정보를 주는 것이며, 더 나아가 고농도 사포닌성분이 함유된 기능성 홍삼제품 개발에 기초정보를 제공하는 결과라 사료된다.

흑미 왕겨 추출물을 이용한 견직물의 천연염색 (Natural Dyeing of Silk Fabrics with Black Rice Bran Extract)

  • 이근숙;배도규
    • Current Research on Agriculture and Life Sciences
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    • 제27권
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    • pp.13-19
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    • 2009
  • The purpose of this study was the efficient use of the black rice bran for dyeing textiles. For this purpose, we investigated proper extracting conditions of black rice bran, dyeability and dyeing fastness of dyed silk fabrics. To find proper extracting condition of black rice bran, we extracted black rice bran with water at different temperatures($40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$), different extracting pH(pH3, pH4, pH5, pH6) and extracting time(20, 40, 60, 80, 100, 120, 140, 160, 180min.). Also we investigated the effect of dyeing time(1, 2, 3, 4, 5, 6hr.), dyeing temperature($40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$) and mordanting method(non, pre, sim, post) to examine dyeability and dyeing fastness of dyed silk fabrics. As a result, when the extracting temperature and time were $80^{\circ}C$, 3hr., respectively, extracting was best. And the higher the extracting concentration, the more the extracting amount. As the dyeing temperature and time were higher and longer, the dyeability of silk fabrics was better. With mordant, the dyeability was improved and when using premordant method better, the K/S value was maximized. The laundering fastness of the silk fabrics dyed with black rice bran was estimated to have a good grade of 3~4, however, the light fastness was poor to have a grade of 1~2.

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종류별 커피의 볶음 및 추출조건에 따른 품질 특성에 관한 연구 (A Study of the Characteristics of Different Coffee Beans by Roasting and Extracting Condition)

  • 김하경;황성연;윤수봉;천덕상;공석길;강근옥
    • 한국식품영양학회지
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    • 제20권1호
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    • pp.14-19
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    • 2007
  • 본 연구는 커피의 품질에 큰 영향을 미치는 카페인, 산도 등을 알아보기 위하여 생산지별 커피 생두를 로스팅 단계에 따른 명도 차이 및 추출 온도 조건에 따른 pH, 산도, 고형분, 카페인 함량 등에 대하여 조사하였는데 그 결과는 다음과 같았다. 추출온도에 따른 pH는 원산지별, 종류별로 큰 차이를 보이지 않았으며, 산도의 경우 추출온도가 낮을수록 수치가 높게 나타나는 경향을 보였는데, 특히 에티오피아 커피와 인도네시아 커피의 산도가 높게 나타났다. 고형분 함량 변화의 경우 원두 내부의 세포구조가 완전히 팽창되어 있는 상태에서는 추출액이 쉽게 통과하므로 추출온도가 빠르게 떨어지지 않아 고형분의 함량이 증가하는 경향을 보였는데, 커피 중 콜롬비아 커피의 고형분 함량이 전체 추출온도에서 모두 가장 높게 나타났다. 또한 볶은 원두의 카페인 함량에서는 콜롬비아, 에티오피아, 브라질 커피의 함량이 각각 11.19, 12.08, 12.56 mg/g으로 나타났으며, 이는 인도네시아, 베트남 커피의 23.15, 22.36 mg/g보다 2배 정도 낮은 것으로 나타났다. 그리고 추출온도별 카페인 함량을 분석한 결과 낮은 온도에 비해 높은 온도에서 추출할 경우, 카페인 함량이 증가하는 추세를 보였다. 이상의 결과에서 arabica 종인 콜럼비아 커피가 카페인 함량은 적은 반면 향미는 더 풍부하여 다른 품종의 커피보다 맛이 더 좋은 것으로 나타났다.

Purification of Isoflavone from Soybean Hypocotyl Using Different Solvents

  • Park, Yeon-Bae;Kim, Min-Jung;Lee, Yoon-Bak;Sohn, Heon-Soo;Lee, Ok-Hee;Kim, Kang-Sung
    • 환경생물
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    • 제21권3호
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    • pp.245-250
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    • 2003
  • Composition of isoflavone in cotyledon and hypocotyl of soybean were detected using HPLC. Optimum conditions for extracting isoflavone from hypocotyl were studied as well. Contents of isoflavone in soybean cotyledon and hypocotyl were 482.5 mg 100 $g^{-1}$ and 3453.3 mg 100 100 $g^{-1}$, respectively. Hypocotyl contained 7~8 times move isoflavone than corresponding cotyledon of the soybean. Malonyl glycoside accounted for move than 70% of the total isoflavone, followed by glycoside, acetyl glycoside, and aglycone. Aqueous ethanol of 60~80% was the most suitable solvent for extracting isoflavone from the hypocotyl. Optimum temperature and time was $90^{\circ}C$, 1hr. Acetic acid, NaCl, and NaOH added to 80% ethanol suppressed extraction yield of the phytochemieal.

Identifying Responsive Functional Modules from Protein-Protein Interaction Network

  • Wu, Zikai;Zhao, Xingming;Chen, Luonan
    • Molecules and Cells
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    • 제27권3호
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    • pp.271-277
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    • 2009
  • Proteins interact with each other within a cell, and those interactions give rise to the biological function and dynamical behavior of cellular systems. Generally, the protein interactions are temporal, spatial, or condition dependent in a specific cell, where only a small part of interactions usually take place under certain conditions. Recently, although a large amount of protein interaction data have been collected by high-throughput technologies, the interactions are recorded or summarized under various or different conditions and therefore cannot be directly used to identify signaling pathways or active networks, which are believed to work in specific cells under specific conditions. However, protein interactions activated under specific conditions may give hints to the biological process underlying corresponding phenotypes. In particular, responsive functional modules consist of protein interactions activated under specific conditions can provide insight into the mechanism underlying biological systems, e.g. protein interaction subnetworks found for certain diseases rather than normal conditions may help to discover potential biomarkers. From computational viewpoint, identifying responsive functional modules can be formulated as an optimization problem. Therefore, efficient computational methods for extracting responsive functional modules are strongly demanded due to the NP-hard nature of such a combinatorial problem. In this review, we first report recent advances in development of computational methods for extracting responsive functional modules or active pathways from protein interaction network and microarray data. Then from computational aspect, we discuss remaining obstacles and perspectives for this attractive and challenging topic in the area of systems biology.

A Study on Extraction Conditions of Paeonia lactiflora for High Immunostimulatory Activity

  • Jeong Won Choi;Hyeok Jin Choi;Gwang Hyeon Ryu;Seung Woo Im;Jae Won Lee;Jin Boo Jeong
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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    • pp.42-42
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    • 2023
  • Paeonia lactiflora roots (PLR) are a traditional medicinal plant used to treat inflammatory diseases. Recently, PLR has been reported to increase the secretion of immune regulatory factors and enhance phagocytic activity in macrophages. Therefore, in this study, we compared the macrophage activation induced by PLR under different extraction conditions. PLR extracts at temperatures ranging from 4℃ to 60℃ increased the secretion of immune regulatory factors, but the secretion slightly decreased at 80℃. Under time-based extraction conditions at 60℃, immune regulatory factor secretion by PLR extracts was similar from 1 to 24 hours. Therefore, considering the overall results of this study, extracting PLR at 60℃ for 1 hour is considered the optimal condition for macrophage activation.

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염료추출 및 분석 조건에 따른 꼭두서니의 색소성분 분리 거동 (Separation of Chromophoric Substance from Madder Plant under Different Extraction and Analytical Conditions)

  • 안춘순
    • 한국의류학회지
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    • 제27권11호
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    • pp.1350-1357
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    • 2003
  • This research was aimed to establish the standard extraction and analytical procedures for examining the chromophoric substance in madder root with the ultimate goal of identifying the dyes in badly faded textiles of archaeological origin. The separation temperature of gas chromatography, pH and other extraction conditions were tested. The results were as follows: The suitable separation temperature for the GC cappillary column was 50∼305$^{\circ}C$, and methanol was a good GC solvent for both standard alizarin and madder extraction. The best extraction of madder was achieved by 90 min soaking in room temperature followed by filtration and the actual heat extraction procedure. The best pH for extracting alizarin was pH 3 and above pH 5 alizarin was not detectible. Only alizarin and no purpurin was found in the extraction of the currently used madder plant.