• 센서학회지
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• 제23권1호
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• pp.35-41
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• 2014

Microbes have been used extensively in various fields of researches and industries but has not been used widely for microfluidic biosensor applications because it is difficult to immobilize properly to a small space. Therefore, we developed a microbial immobilization method for microfluidic devices using single-walled nanotubes and dielectrophoretic force. Single-walled nanotubes and Escherichia coli were aligned between two cantilever electrodes by a positive dielectrophoretic force resulting in a film of single-walled nanotubes with attached Escherichia coli. The optimal condition of film formation without a cell lysis was investigated. Diameter of single-walled nanotubes and electric field (intensity and duration of application) had an effect on the cell viability. On the other hand, the cell concentration of the suspension did not affect the cell viability. Paraoxon was detected using single-walled nanotubes film with attached Escherichia coli that expressed organophosphorus hydrolase. This film which is suspended from the substrate showed faster response time than sensors that are not suspended from the substrate.

• 한국분말재료학회지
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• 제17권4호
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• pp.326-335
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• 2010

In this paper, the effect of electrical characteristics and electrode shape on the alignment and attachment of multi-walled carbon nanotubes (MWNTs) has been studied. The attraction and alignment of MWNTs between the gaps has been investigated by applying electric field which is called electrophoresis and dielectrophoresis. According to the frequency of electric field, positive or negative dielectrophoretic force can be determined. The concentration of MWNTs solution was $5\;{\mu}g/ml$, and a droplet of $1.0{\sim}1.5\;{\mu}l$ was dropped between two electrodes. Through the repeated experiments, the optimal electrical conditions for aligning and attaching MWNTs in the desired places were obtained. Since the frequency range of 100 kHz~10 MHz generated positive dielectrophoretic force, MWNTs were attracted and aligned between the gaps with this frequency range. For generating enough force to attract MWNTs, the appropriate voltage range was $0.3{\sim}1.3\;V_{rms}/{\mu}m$. Furthermore, the effect of electrode shape on the alignment of MWNTs was investigated. A single MWNT attachment was accomplished on the round shaped with 70% yield.

• 대한의용생체공학회:의공학회지
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• 제34권4호
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• pp.189-196
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• 2013

Dielectrophoresis(DEP) is useful in manipulation and separation of micro-sized particles including biological samples such as bacteria, blood cells, and cancer cells in a micro-fluidic device. Especially, those separation and manipulation techniques using DEP in combination of micro fabrication technique have been researched more and more. Recently, it is revealed that a window structure of insulating layer in microfluidic DEP chip is key role in trap of micro-particles around the window structure. However, the trap phenomenon-driven by DEP force gradient did not fully understand and is still illusive. In this study, we characterize the trap mechanism and efficiency with different shapes of window in a microfluidic DEP chip. To do this characterization, we fabricated 4 different windows shapes such as rhombus, circle, squares, and hexagon inside a micro-fluidic chip, and performed micro-sized particles manipulation experiments as varying the frequency and voltage of AC signal. Moreover, the numerical simulation with the same parameters that were used in the experiment was also performed in order to compare the simulation results and the experimental results. Those comparison shows that both results are closely matched. This study may be helpful in design and development of microfluidic DEP chip for trapping micro-scaled biological particle.

• 한국정밀공학회지
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• 제24권3호
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• pp.134-139
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• 2007

We report the suspension of individual multi-walled carbon nanotubes (MWNTs) from the bottom substrate using deep trench electrodes that were fabricated using optical lithography. During drying of the solution in dielectrophoretic assembly, the capillary force pulls the MWNT toward the bottom substrate, and it then remains as a deformed structure adhering to the bottom substrate after the solution has dried out. Small-diameter MWNTs cannot be suspended using thin electrodes with large gaps, but large-diameter MWNTs can be suspended using thicker electrodes. We present the statistical experimental results for successful suspension, as well as the feasible conditions for a MWNT suspension based on a theoretical approach.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.501-504
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• 2004

This paper describes the fabrication method for atomic force microscopy(AFM) tip with multi-walled carbon nanotube(MWNT). For making a carbon nanotube (CNT) modified tips, AC electric field which cause the dielectrophoresis was used for alignment and deposition of CNTs in this research. By dropping the MWNT solution and applying an electric field between an AFM tip and an electrode, MWNTs which were dispersed into a diluted solution were directly assembled onto the apex of the AFM tips due to the attraction by the dielectrophoretic force. In this case, we investigate the effect of the angle between a tip axis and an electrode. Experimental setup were presented, and then CNT attached AFM tips are successfully shown in this paper.

• 전기학회논문지P
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• 제66권3호
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• pp.139-143
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• 2017

In this paper, we propose a separation method of the dielectric particles in the liquid flow. Since particles are dielectric in most cases, they experience dielectrophoretic(DEP) force under non-uniform electric field. The field characteristics in the electromagnetic and fluid dynamic systems are solved by using the finite element method. The motional equation of the particles is calculated by the Runge-Kutta method. The field analysis shows the feasibility of the proposed method. The particle separation model with large DEP force exerting on particles is designed by analyzing field characteristics.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1687-1689
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• 2008

In the detection of pathogenic microorganisms ATP-bioluminescence reaction is a fascinating method. ATP(adenosine triphosphate) is an energy source of all kinds of living organism and ATP-bioluminescence reaction uses this ATP. However, ATP exists not only in the cells but also outside the cells. Therefore ATP-bioluminescence reaction only with intracellular ATP is very important in pathogenic microorganism detection. Because of that reason we developed a microfluidic channel containing Dielectrophoretic zone which capture microorganisms and eliminating and washing extracellular ATP with ATP-degarading enzymes, adenosine phosphate deaminase and apyrase. Microorganisms are captured by pDEP force at the DEP electrode zone and only extracellular ATPs are washed and eliminated outside the zone.

• 대한기계학회논문집A
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• 제33권1호
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• pp.49-55
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• 2009

This paper presents the optimal shape of microelectrode that generates dielectrophoretic(DEP) force to separate particles in homogeneous medium. The principle of the particles sorting is based on the use of the relative strengths of negative DEP (nDEP) and drag forces, as in a general DEP-activated cell sorter (DACS). To numerically calculate the DEP force and drag force, the simulation is implemented in MATLAB 7.0. The properties of particles, which are used in simulation, are similarly selected as those of cells to apply cell separation. The most optimized shape of electrode is selected by numerical simulation according to a variety of electrode shape such as rectangle, trapezoidal, and right-triangle. Through, in addition, parameter study, we found that applied frequency is more significant factor on the separation than various parameters, such as applied voltage and permittivity of medium, that decide on the strength of DEP force.

• 대한기계학회논문집B
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• 제39권5호
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• pp.425-433
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• 2015

본 논문에서는 외부에서 균일한 직류전기장이 인가될 때 점성유체에 자유롭게 잠겨있는 단일 입자가 근처의 비전도성 평면 벽과의 상호작용 때문에 유발되는 2차원 유전영동 운동에 대하여 수치연구를 수행하였다. 특히 입자-유체 경계면에서 불연속적으로 급격히 변화하는 전기전도도를 가진 Maxwell 방정식을 해석하고 전기장을 구한 후 Maxwell 응력텐서를 적분하여 입자에 작용하는 유전영동 힘을 계산하였다. 해석 결과 전기장이 벽과 평행하게 인가될 때 입자는 항상 반발력이 유도되어 벽으로부터 멀어지는 방향으로 유전영동 운동이 발생하였으며, 그 운동특성은 입자와 벽 사이 간격과 입자의 전도도에 따라 크게 달라졌다. 운동 강도는 입자와 유체의 전도도가 서로 같으면 사라지나, 전도도가 서로 다르면 그 차이가 클수록 강도는 증가하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 C
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• pp.1445-1447
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• 1995

A cell fusion device is designed and fabricated in order to electrofuse two cells between electrodes. Dielectrophoretic force is used to attract each cell and make a pearl chain of two cells. Two kinds of electrode structure are fabricated and tested the feasibility of the proposed device. The attraction of two radish cells or two Chinese cabbage cells on the electrodes is observed when AC voltage(1MHz, $V_{p-p}$=8V) is applied to the electrodes. The possibility of cell fusion device is shown through experiments.