• Title/Summary/Keyword: Degenerate

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Search for [NiFe]-Hydrogenase using Degenerate Polymerase Chain Reaction (Degenerate Polymerase Chain Reaction을 통한 [NiFe]-Hydrogenase의 탐색)

  • Jung, Hee-Jung;Kim, Jaoon Y.H.;Cha Hyung-Joon
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.11a
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    • pp.631-633
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    • 2005
  • For biohydrogen production, hydrogenase is a key enzyme. In the present work we performed search of [NiFe]-hydrogenases from hydrogen producing microorganisms using degenerate polymerase chain reaction (PCR) strategy. Degenerate primers were designed from the conserved region of [NiFe]-hydrogenase group I especially on structural genes encoding for catalytic subunit of [NiFe]-hydrogenase from bacteria producing hydrogen. Most of [NiFe]-hydrogenase (group I) are expressed via complex mechanism with aid of auxiliary protein and localized through twin-arginine translocation pathway. [NiFe]-hydrogenase is composed of large and small subunits for catalytic activity. It is known that only small subunit has signal peptide for periplasmic localization and large & small subunitscome together before localization. During this process, large subunit is treated by endopeptidase for maturation. Based on these information we used signal peptide sequence and C-terminal of large subunit by recognized by endopeptidase as templates for degenerate primers. About 2,900 bp of PCR products were successfully amplified using the designed degenerate primers from genomic DNAs of several microorganisms. The amplified PCR products were inserted into T-vector and then sequenced to confirm.

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New Degenerate Primer for the Cyanobacterial Non-ribosomal Peptides (시아노박테리아 Non-ribosomal Peptides의 효과적인 연구를 위한 New Degenerate Primer의 개발)

  • Kim, Gi-Eun
    • KSBB Journal
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    • v.22 no.5
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    • pp.362-365
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    • 2007
  • Cyanobacteria have been identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Large multienzyme complexes which are responsible for the non-ribosomal biosynthesis of peptides are modular for the addition of a single amino acid. An activation of amino acid substrates results in an amino adenylate occuring via an adenylation domain (A-domain). A-domains are responsible for the recognition of amino acids as substrates within NP synthesis. The A-domain contains ten conserved motifs, A1 to A10. In this study, ten conserved motifs from A1 to A10 were checked regarding their amino acid sequence of the NRPS-module of Microcystis aeruginosa PCC 7806. The part of the amino acid sequence chosen was that which contained as many conserved motives as possible, and then these amino sequence were compared between other cyanobacteria to design a new degenerate primer. A new degenerate primer (A3/A7 primer) was designed to detect any putative NP synthetase region in unkwon cyanobacteria by a reverse translation of the conserved amino acid sequence and a search for cyanobacterial DNA bank.

Degenerate Weakly (k1, k2)-Quasiregular Mappings

  • Gao, Hongya;Tian, Dazeng;Sun, Lanxiang;Chu, Yuming
    • Kyungpook Mathematical Journal
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    • v.51 no.1
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    • pp.59-68
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    • 2011
  • In this paper, we first give the definition of degenerate weakly ($k_1$, $k_2$-quasiregular mappings by using the technique of exterior power and exterior differential forms, and then, by using Hodge decomposition and Reverse H$\"{o}$lder inequality, we obtain the higher integrability result: for any $q_1$ satisfying 0 < $k_1({n \atop l})^{3/2}n^{l/2}\;{\times}\;2^{n+1}l\;{\times}\;100^{n^2}\;\[2^l(2^{n+3l}+1)\]\;(l-q_1)$ < 1 there exists an integrable exponent $p_1$ = $p_1$(n, l, $k_1$, $k_2$) > l, such that every degenerate weakly ($k_1$, $k_2$)-quasiregular mapping f ${\in}$ $W_{loc}^{1,q_1}$ (${\Omega}$, $R^n$) belongs to $W_{loc}^{1,p_1}$ (${\Omega}$, $R^m$), that is, f is a degenerate ($k_1$, $k_2$)-quasiregular mapping in the usual sense.

Femtosecond degenerate and nondegenerate pump-probe experiments in bulk GaAs below the band gap

  • Yahng, J. S.;Kim, D. S.;Fatti, N.Del;Vallee, F.
    • Journal of the Optical Society of Korea
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    • v.1 no.2
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    • pp.100-103
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    • 1997
  • We perform degenerate and nondegenerate pump-probe experiments on bulk GaAs at 100 K below the band gap. We mostly observe a negative differential transmission signal both in the degenerate and nondegenerate experiments. We interpret our signal as due to two-photon absorption. This negative signal has a different origin from the normally considered band gap renormalization for resonant excitations.

REGULARITY OF A DEGENERATE PARABOLIC EQUATION APPEARING IN VECER'S UNIFIED PRICING OF ASIAN OPTIONS

  • Dong, Hongjie;Kim, Seick
    • Bulletin of the Korean Mathematical Society
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    • v.52 no.3
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    • pp.947-953
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    • 2015
  • Vecer derived a degenerate parabolic equation characterizing the price of Asian options with generally sampled average. It is well understood that there exists a unique probabilistic solution to Vecer's PDE but it remained unclear whether the probabilistic solution is a classical solution. We prove that the probabilistic solution to Vecer's PDE is indeed regular.

Linear Algorithm for Motion Estimation from Point Correspondences over Two Perspective Views: How to Handle Degenerate Cases (선형해석버버에 의한 점대응 움직임 추정 연구 : Degenerate Case에 대한 대책에 관하여)

  • Shim, Young-Serk
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.11
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    • pp.1850-1856
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    • 1989
  • For determining motion/structure of a 3-D rigid object from point correspondences over two perspective views, a linear algorithm was developed in Refs. 3 and 4. This algorithm fails when the 3-D points under observation satisfy certain geometrical constraints, as demonstrated in Refs. 7 and 8. In the present paper, we show that the linear algorithm can be resurrected in these degenerate cases by adding additional lower order polynomial constraints to original equations.

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UNIQUENESS OF SOLUTIONS FOR A DEGENERATE PARABOLIC EQUATION WITH ABSORPTION

  • Lee, Jin Ho;Jang, Seong Hee
    • Korean Journal of Mathematics
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    • v.5 no.2
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    • pp.151-167
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    • 1997
  • We estimate the interior Lipschitz norm and maximum of the solution for degenerate parabolic equations with absorption. Also obtain the growth rate of the solution $u$ in terms of time $t$. From this we show the uniqueness of solution with respect to the initial trace.

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CRITICAL EXPONENTS FOR A DOUBLY DEGENERATE PARABOLIC SYSTEM COUPLED VIA NONLINEAR BOUNDARY FLUX

  • Mi, Yongsheng;Mu, Chunlai;Chen, Botao
    • Journal of the Korean Mathematical Society
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    • v.48 no.3
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    • pp.513-527
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    • 2011
  • The paper deals with the degenerate parabolic system with nonlinear boundary flux. By constructing the self-similar supersolution and subsolution, we obtain the critical global existence curve. The critical Fujita curve is conjectured with the aid of some new results.