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Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates

  • Jiao-Jiao Chen;Zhang Zhang;Yi Wang;Xiao-Long Yuan;Juan Wang;Yu-Ming Yang;Yuan Zheng
    • Mycobiology
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    • v.51 no.1
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    • pp.49-59
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    • 2023
  • Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzoquinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM compared to the other two wood substrates. Similarly, the expression of geranylgeranyltransferase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.

Membrane Contactors for Water Carbonation

  • Alessandra Criscuoli;Enrico Drioli
    • Membrane Journal
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    • v.1 no.1
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    • pp.59-59
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    • 1991
  • A theoretical and experimental study made in order to determine the performance of mem-brane contactors in water carbonation is presented. In particular on the basis of experimental results pre-viously obtained it has been derived an expression in which the effect of some parameters as temprera-ture water and CO₂ flow rate CO₂ pressure trans-membrane pressure on the performance of the process is taken into account. The study refers to hollow fiber membrane contactors used for the experimental tests. The main scope has been to verify if by membrane contactors it is possible to reach the same de-gree of water carbonation as by trditional methods (1-5 g/1) and to derive for the module used a cor-relation able to describe the performance of the process at several operating conditions. The high CO₂ removal observed confirms the interesting potentialties of membrane contactors also in gas streams purification.

Color tunable electroluminescence with polymer blends composed of PVK and copolymer containing SiPh-PPV and MEH-PPV unit

  • Oh, Gwang-Chae;Yun, Je-Jung;Park, Su-Mi;Son, Sung-Hee;Han, Eun-Mi;Jin, Sung-Ho;Gu, Hal-Bon;Choi, Hyun-Chual
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.736-739
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    • 2002
  • We report on white light emission from a light emitting diode(LED) prepared by blending a red emitting copolymer, m-SiPh PPV-co-MEH PPV, and a blue emitting polyvinylcarbazole (PVK). White light emission was realized when the weight ratio of the m-SiPh PPV-co-MEH PPV : PVK equals to 1 : 30, in which the commission Internationale de L'Eclairage coordinates were x=0.3266 and y=0.3438.

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Membrane Contactors for Water Carbonation

  • Alessandra Criscuoli;Enrico Drioli
    • Korean Membrane Journal
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    • v.1 no.1
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    • pp.59-64
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    • 1999
  • A theoretical and experimental study made in order to determine the performance of mem-brane contactors in water carbonation is presented. In particular on the basis of experimental results pre-viously obtained it has been derived an expression in which the effect of some parameters as temprera-ture water and {{{{{CO }_{2 } }}}}} flow rate {{{{{CO }_{2 } }}}}} pressure trans-membrane pressure on the performance of the process is taken into account. The study refers to hollow fiber membrane contactors used for the experimental tests. The main scope has been to verify if by membrane contactors it is possible to reach the same de-gree of water carbonation as by trditional methods (1-5 g/1) and to derive for the module used a cor-relation able to describe the performance of the process at several operating conditions. The high {{{{{CO }_{2 } } }}}} removal observed confirms the interesting potentialties of membrane contactors also in gas streams purification.

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Kinematics of the Envelope and Two Bipolar Jets in L1157

  • Kwon, Woojin;Fernandez-Lopez, Manuel;Stephens, Ian W.;Looney, Leslie W.
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.72.3-72.3
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    • 2016
  • A massive envelope and a strong bipolar outflow are the two most distinct structures of youngest protostellar systems. We present observational results from the Combined Array for Research in Millimeter-wave Astronomy (CARMA) toward the youngest (Class 0) protostellar system L1157. At an angular resolution of 5 arcseconds, we mapped its well-developed outflow in CO 2-1 over a span of approximately 5 arcminutes. Additionally, we imaged the central envelope with CO isotopes, CS, CN, and N2H+ with an angular resolution of about 2 arcseconds. We show that the bipolar outflow may be represented with a two jet model and constrain its physical properties such as precession/rotation directions, velocities, inclinations, and position angles via cube data fitting. In addition, we discuss the kinematic features of the envelope detected in CO isotopes and N2H+ and present the radius-dependent dust opacity spectral index.

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Flexible Liquid Crystal Film Using Continuous Process

  • Liao, Chi-Chang;Wang, Hsing-Lung;Liu, Kang-Hung;Chen, Ru-De;Chang, Chih-Yuan;Jeng, Shie-Chang;Lin, Yan-Rung;Lu, Kevin;Chang, Rick;Chen, Jerry
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08a
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    • pp.73-75
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    • 2007
  • Micro-cell LC film and polymer network liquid crystal (PNLC) film by using continuous compatible process have been developed . A high-contrast micro-cell LC film has a strong potential as a high-performance flexible device. PNLC film has the low driving voltage. Both films show the characteristics of lightness, thinness and mechanical stability.

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Intraoperative pulmonary embolism in shoulder arthroscopy in a patient with previous SARS-CoV-2 infection: a case report

  • de Lizana, Alejandro Holgado-Rodriguez;Izquierdo-Fernandez, Alberto;Mendez-Sanchez, Jesus Manuel;Diaz-Minarro, Jose Carlos
    • Clinics in Shoulder and Elbow
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    • v.25 no.3
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    • pp.236-239
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    • 2022
  • The objective of this article is to describe intraoperative pulmonary embolism during shoulder arthroscopy in a patient with previous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Further, we describe how the pandemic has influenced the population by increasing the rate of embolisms. Awareness of such cases will help to increase knowledge regarding SARS-Cov-2 and to determine if such patients should receive routine antithrombotic prophylaxis.

Effect of Hydrocarbon Additives on SNCR DeNOx Characteristics under Oxidizing Diesel Exhaust Gas Conditions

  • Nam, Changmo
    • Journal of Environmental Science International
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    • v.27 no.10
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    • pp.809-820
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    • 2018
  • DeNOx experiments for the effects of hydrocarbon additives on diesel SNCR process were conducted under oxidizing diesel exhaust conditions. A diesel-fueled combustion system was set up to simulate the actual cylinder and head, exhaust pipe and combustion products, where the reducing agent $NH_3$ and $C_2H_6/diesel$ fuel additives were separately or simultaneously injected into the exhaust pipe, used as the SNCR flow reactor. A wide range of air/fuel ratios (A/F=20~40) were maintained, based on engine speeds where an initial NOx level was 530 ppm and the molar ratios (${\beta}=NH_3/NOx$) ranged between 1.0~2.0, together with adjusting the amounts of hydrocarbon additives. Temperature windows were normally formed in the range of 1200~1350K, which were shifted downwards by 50~100K with injecting $C_2H_6/diesel$ fuel additives. About 50~68% NOx reduction was possible with the above molar ratios (${\beta}$) at the optimum flow #1 ($T_{in}=1260K$). Injecting a small amount of $C_2H_6$ or diesel fuel (${\gamma}=hydrocarbon/NOx$) gave the promising results, particularly in the lower exhaust temperatures, by contributing to the sufficient production of active radicals ($OH/O/HO_2/H$) for NOx reduction. Unfortunately, the addition of hydrocarbons increased the concentrations of byproducts such as CO, UHC, $N_2O$ and $NO_2$, and their emission levels are discussed. Among them, Injecting diesel fuel together with the primary reductant seems to be more encouraging for practical reason and could be suggested as an alternative SNCR DeNOx strategy under diesel exhaust systems, following further optimization of chemicals used for lower emission levels of byproducts.

Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Recombinant E. coli (재조합 대장균으로부터 항고혈압 Angiotensin I-Converting Enzyme 저해제의 특성연구)

  • Kim, Jae-Ho;Jeong, Seung-Chan;Lee, Dae-Hyong;Lee, Jong-Soo
    • The Journal of Natural Sciences
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    • v.16 no.1
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    • pp.1-13
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    • 2005
  • The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. Coli BL21(DE3) harboring the recombination pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.

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Thorea indica sp. nov. (Thoreales, Rhodophyta) from Uttar Pradesh, India

  • Necchi, Orlando Jr;Paiano, Monica O.;West, John A.;Ganesan, E. K.;Goer, Susan Loiseaux-de
    • ALGAE
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    • v.30 no.4
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    • pp.265-274
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    • 2015
  • Thorea indica sp. nov. is described from the Sai River, Uttar Pradesh, India (26°39′00.7″ N, 80°47′38.3″ E). Its classification is based on molecular sequences of the plastid-encoded RuBisCO large-subunit gene, rbcL and the barcode region of the mitochondrial encoded cytochrome c oxidase subunit 1, cox1, and morphological data. The sequence analyses confirm a new species of Thorea. The cox1 barcode sequence had 90.4-90.8% identity with Thorea sp. from Australia and Thorea hispida from Hawaii and China. Based on rbcL sequences the Indian specimen was positioned in a major clade with high support (>95 bootstrap and 0.95 posterior probability) containing two other species: T. okadae from Japan and T. hispida from the continental USA, Hawaii, the UK, and China. The divergences among these sequences were T. indica vs. T. okadae (2.8%) and T. indica vs. T. hispida (2.9-3.4%). The comparison of morphological characters of Thorea from India was not conclusive due to the inadequate descriptions in previous reports: most specimens reported as T. hispida fit within the circumscription of T. indica as described here. The previous report of T. siamensis from the Sai River is incorrect and the specimens fit within our description of T. indica. Thorea indica and T. okadae can be distinguished by minor morphometric characters and sexuality (dioecious vs. monoecious).