Mycobiology

The Korean Society of Mycology (한국균학회)
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Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates

  • Jiao-Jiao Chen (College of Forestry, Southwest Forestry University) ;
  • Zhang Zhang (College of Forestry, Southwest Forestry University) ;
  • Yi Wang (Yunnan Key Laboratory of Forest Plant Cultivation, Development and Utilization, Yunnan Academy of Forestry and Grassland) ;
  • Xiao-Long Yuan (Yunnan Key Laboratory of Forest Plant Cultivation, Development and Utilization, Yunnan Academy of Forestry and Grassland) ;
  • Juan Wang (Institute of Green Development, Southwest Forestry University) ;
  • Yu-Ming Yang (Institute of Green Development, Southwest Forestry University) ;
  • Yuan Zheng (College of Forestry, Southwest Forestry University)
  • Received : 2022.11.07
  • Accepted : 2023.01.27
  • Published : 2023.02.28
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Abstract

Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzoquinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM compared to the other two wood substrates. Similarly, the expression of geranylgeranyltransferase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.

Keywords

Acknowledgement

The authors are grateful for the support of the National Natural Science Foundation of China [No. 32160736, 31860177]; Major Project of the Agricultural Basic Research Program in Yunnan Province [202101BD070001-020]; General Project of the Basic Research Program in Yunnan Province [202101AT070218, 202101AT070044]; the Reserve Talents for Young and Middle-aged Academic and Technical Leaders of the Yunnan Province [202205AC160044]; Yunnan Key Laboratory for Fungal Diversity and Green Development [E03A311261-3].

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