• Archives of Pharmacal Research
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• 제28권8호
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• pp.923-929
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• 2005

Methanol extracts of domestic plants of Korea were evaluated as a potential inhibitor of neutral pH optimum and membrane-associated 60 kDa sphingomyelinase (N-SMase) activity. In this study, we partially purified N-SMase from bovine brain membranes using ammonium sulfate. It was purified approximately 163-fold by the sequential use of DE52, Butyl-Toyopearl, DEAE-Cellulose, and Phenyl-5PW column chromatographies. The purified N-SMase activity was assayed in the presence of the plant extracts of three hundreds species. Based on the in vitro assay, three plant extracts significantly inhibited the N-SMase activity in a time- and concentration-dependent manner. To further examine the inhibitory pattern, a Dixon plot was constructed for each of the plant extracts. The extracts of Abies nephrolepis, Acer tegmentosum, and Ginkgo biloba revealed a competitive inhibition with the inhibition constant (Ki) of $11.9 {\mu}g/mL,\;9.4{\mu}g/mL,\;and\;12.9{\mu}g/mL$, respectively. These extracts also inhibited in a dose-dependent manner the production of ceramide induced by serum deprivation in human neuroblastoma cell line SH-SY5Y.

• 미생물학회지
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• 제38권1호
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• pp.45-49
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• 2002

Bradyrhizobium japonicum 은 콩과 식물의 뿌리에 감염하여 뿌리흑을 형성 질소를 고정하는 독특한 능력을 갖는 토양 세균이며 미생물 비료제로 사용되고 있다. 본 연구에서는 저온에서 전처리한 B . japonicum 균주를 여러 가지 환경스트레스 조건에 노출하였을 때 생균수의 변화를 확인하였다. 저온 전처리는 16시간 동안 $4^{\circ}C$의 조건을 유지했다. 다양한 스트레스(알콜, 과산화수소, 고온, 건조)에 노출하였을 때, 저온 전처리한 것이 그렇지 않는 것보다 10~1,000배 정도 높은 생균수를 유지하였다. 이러한 내성중진 현상에 전처리 동안 새로운 단백질 합성이 수반되는 것을 단백질 합성 저해제 인 chloramphenicol을 전처리 과정에 포함하여 확인하였다. 저온 스트레스 내성에 관여하는 유전자를 B. japonicum genome 으로부터 중폭하였고 염기서열 분석을 실시하였다. 실험에서 확인된 B . japonicum의 CSP (Cold shock protein) 단백질의 부분적 아미노산 서열은 이미 확인된 다른 균주의 Csp 단백질과 유사함을 확인하였다.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.189-195
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• 2020

The soybean (Glycine max L.), also known as the soya bean, is an economically important legume species. Pathogens are always major threats for soybean cultivation. Several pathogens negatively affect soybean production. The soybean is also known as a susceptible host to many viruses. Recently, we carried out systematic analyses to identify viruses infecting soybeans using soybean transcriptome data. Our screening results showed that only few soybean transcriptomes contained virus-associated sequences. In this study, we further carried out bioinformatics analyses using a soybean flower bud transcriptome for virus identification, genome assembly, and single nucleotide variations (SNVs). We assembled the genome of Soybean yellow common mosaic virus (SYCMV) isolate China and revealed two SNVs. Phylogenetic analyses using three viral proteins suggested that SYCMV isolate China is closely related to SYCMV isolates from South Korea. Furthermore, we found that replication and mutation of SYCMV is relatively low, which might be associated with flower bud tissue. The most interesting finding was that SYCMV was not detected in the cytoplasmic male sterility (CMS) line derived from the non-CMS line that was severely infected by SYCMV. In summary, in silico analyses identified SYCMV from the soybean flower bud transcriptome, and a nearly complete genome of SYCMV was successfully assembled. Our results suggest that the low level of virus replication and mutation for SYCMV might be associated with plant tissues. Moreover, we provide the first evidence that male sterility might be used to eliminate viruses in crop plants.

• 한국융합학회논문지
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• 제8권3호
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• pp.49-61
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• 2017

안드로이드 플랫폼에서 다양한 모바일 애플리케이션이 개발/배포되면서 편리함과 유용성이 더욱 증가하고 있으나 지속적으로 악성 모바일 애플리케이션(Malicious Mobile Application) 또한 급증하고 있어 스마트폰 사용자도 모르게 단말 내 중요 정보 등이 외부로 유출되고 있다. 악성앱 검출을 위해 안드로이드 플랫폼을 대상으로 다양한 모바일 백신이 개발되었지만 최근에 발견된 서버 기반 다형상 모바일 악성앱인 경우 은닉 우회 기법을 포함하고 있으며, C&C 서버 기반 다형상 생성기에 의해서 각 사용자 단말에 매번 조금씩 다른 형태의 악성앱이 생성 및 설치되기 때문에 기존 모바일 백신에 손쉽게 검출되지 않는다는 문제점이 있다. 이에 본 논문에서는 서버 기반 다형상 모바일 악성앱에 대한 APK 역컴파일 과정을 통해 핵심 악성 코드를 구성하는 DEX 파일내 메소드에 대한 유사도와 접근권한 유사도 측정을 통해 상관관계를 분석하여 SSP 악성앱을 판별하는 기법을 제시하였다. DEX 메소드 유사도와 퍼미션 유사도 분석 결과 SSP 악성앱에 대한 동작 방식의 특징을 추출할 수 있었으며 정상앱과 구별 가능한 차이점을 발견할 수 있었다.

• Industrial Engineering and Management Systems
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• 제9권3호
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• pp.285-293
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• 2010

To obtain visible and traceable information from the supply chain, HW/SW standards for the EPC global network, which process electronic product code (EPC) data read from Radio frequency identification (RFID) tags, are regarded as the de facto industry standard. Supply chain participants install information service systems and provide logistics information to partners by following the EPCglobal architecture framework. Although quality of service (QoS) is essential for providing dependable and scalable services as pointed out by Auto-ID Lab, only a few models for the performance analysis of QoS-related work have been developed in the context of EPC information service systems. Specifically, doing so allows alternative design choices to be tested in an easy and cost-effective manner and can highlight potential performance problems in designs long before any construction costs are incurred. Thus, in this study we construct a model of an EPC information service system for the purposes of performance analysis and designing a dependable system. We also develop a set of building blocks for analytical performance models. To illustrate how the model works, we determine the characteristics of an EPC information service system and then select a combination of these proven modeling concepts. We construct a performance model that considers the response time and shows how to derive meaningful performance values. Finally, we compare the analytical results to measurements of the EPC information service system.

• 한국융합학회논문지
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• 제11권3호
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• pp.77-83
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• 2020

최근 지능형 예측감시 시스템이 등장하고 있다. 지능형 예측감시 시스템의 추론을 위해서는 현재 및 과거의 데이터가 필요하며, 이러한 데이터의 분석을 통하여 곧 발생할 상황에 대한 예측을 가능하게 한다. 그러나, 이러한 과정에서 영상 객체의 개인정보를 취급하게 될 소지가 높으므로, 개인정보보호를 위해서는 보안에 대한 고려가 필수적이다. 특히, 개인의 생활패턴, 주요 이동 경로 등에 대한 정보가 해킹을 통하여 공개적으로 노출된다면 프라이버시 측면에서 문제가 될 것이다. 기존의 영상감시 프레임워크는 개인정보보호 측면에서 한계점이 있으며, 특히 개인정보보호에 취약한 측면이 있다. 본 논문에서는 개인정보보호를 고려한 지능형 예측감시 시스템을 위한 보안 프레임워크를 제안하였다. 제안한 방법에서는 단말, 전송, 감시, 모니터링 계층으로 구분하여 단위별 세부 구성요소를 명시하였으며, 특히 객체 단위별 세부 접근제어와 비식별화를 지원하여 영상감시 과정에서의 능동형 개인정보보호가 가능하다. 또한, 데이터 전송시 보안 기능과 RBAC 제공을 통한 접근제어의 장점을 갖는다.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.5-18
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• 2007

Objectives : The purpose of this study was to obtain an objective differentiating method for various types of Panax ginseng: ginseng, cultivated wild ginseng, and natural wild ginseng which are distinctive according to their growing environment. Methods : The roots, stem, and leaves of several types of ginseng were collected and comparative analysis of proteome was conducted on each part using 2-DE and the results examined. Results : 1. Proteome images of the respective parts within the samples showed spot-matching in most cases, suggesting that they are genetically identical panax ginseng. 2. Similar distribution patters were seen within the different parts of the Panax ginseng: ginseng, Chinese cultivated wild ginseng, and the 5 and 10 years old Korean cultivated wild ginseng. 3. For a quantitative evaluation of spots showing differences among the samples, 102 spots from the roots, 109 spots from the stems, and 132 spots form the leaves which showed a difference were selected and centrifugal identification was conducted. 4. Peculiar proteins from each respective part of the Panax ginseng were identified and the top 20 spots with significant differences were selected and analyzed in order to provide a differentiation rate among the samples. The accuracy rate ranged between 23.0-38.8%. 5. Differentiation rate of the top 10 spots with significant differences showed a 50-85% accuracy rate, and the differentiation rate was especially high for the stem of Chinese cultivated wild ginseng and Korean cultivated wild ginseng.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.784-791
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• 2007

The present work aims to use a biofilter technology(aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water(OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days($BOD_5$) and chemical oxygen demand(COD), decreased markedly(up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced(around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the $\alpha-subclass$ of Proteobacteria, and often branched in the periphery of bacteria] genera commonly present in soil(Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1221-1226
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• 2008

The soft rot bacterium Pectobacterium wasabiae is an economically important pathogen of many crops. A new phytase gene, appA, was cloned from P. wasabiae by degenerate PCR and TAIL-PCR. The open reading frame of appA consisted of 1,302 bp encoding 433 amino acid residues, including 27 residues of a putative signal peptide. The mature protein had a molecular mass of 45 kDa and a theoretical pI of 5.5. The amino acid sequence contained the conserved active site residues RHGXRXP and HDTN of typical histidine acid phosphatases, and showed the highest identity of 48.5% to PhyM from Pseudomonas syringae. The gene fragment encoding the mature phytase was expressed in Escherichia coli BL21 (DE3), and the purified recombinant phytase had a specific activity of 1,072$\pm$47 U/mg for phytate substrate. The optimum pH and temperature for the purified phytase were pH 5.0 and 50$^{\circ}C$, respectively. The $K_m$ value was 0.17 mM, with a $V_{max}$ of 1,714 $\mu$mol/min/mg. This is the first report of the identification and isolation of phytase from Pectobacterium.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1235-1244
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• 2008

Indole-3-acetic acid (IAA) is produced commonly by plants and many bacteria, however, little is known about the genetic basis involving the key enzymes of IAA biosynthetic pathways from Bacillus spp. IAA intermediates from the Gram-positive spore-forming bacterium Paenibacillus polymyxa E681 were investigated, which showed the existence of only an indole-3-pyruvic acid (IPA) pathway for IAA biosynthesis from the bacterium. Four open reading frames (ORFs) encoding indole-3-pyruvate decarboxylase-like proteins and putative indole-3-pyruvate decarboxylase (IPDC), a key enzyme in the IPA synthetic pathway, were found on the genome sequence database of P. polymyxa and cloned in Escherichia coli DH5$\alpha$. One of the ORFs, PP2_01257, was assigned as probable indole-3-pyruvate decarboxylase. The ORF consisted of 1,743 nucleotides encoding 581 amino acids with a deduced molecular mass of 63,380 Da. Alignment studies of the deduced amino acid sequence of the ORF with known IPDC sequences revealed conservation of several amino acids in PP2_01257, essential for substrate and cofactor binding. Recombinant protein, gene product of the ORF PP2_01257 from P. polymyxa E681, was expressed in E. coli BL21 (DE3) as a glutathione S-transferase (GST)-fusion protein and purified to homogeneity using affinity chromatography. The molecular mass of the purified enzyme showed about 63 kDa, corresponding closely to the expected molecular mass of IPDC. The indole-3-pyruvate decarboxylase activity of the recombinant protein, detected by HPLC, using IPA substrate in the enzyme reaction confirmed the identity and functionality of the enzyme IPDC from the E681 strain.